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Cloning Pan Florida question


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#1 spacecake

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Posted 17 September 2006 - 03:42 AM

Hi guys...!

I'm going to clone one of my Pans today !
Using 3% peroxide and 2 or 3 parts of one stem.
They will be put in sterilized corn,that after full colonization..will be used the same as a normal colonized jar of corn.

Any comments or idea's ?

I failed the first time using a blender,so this will only be my second try !

#2 alex27

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Posted 17 September 2006 - 03:51 AM

why not try a liquid culture..?

#3 spacecake

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Posted 17 September 2006 - 04:00 AM

why not try a liquid culture..?


I think that will not be suitable for Pans,I don't say it can't be done but if blending them kills them,then I think sucking live culture in a syringe will do the same thing...

And I think that I cannot use a liquid for spawning poo,..so I have to innoculate corn again.. this will take more time that I do not have !

But if you don't agree just say so !

#4 golly

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Posted 17 September 2006 - 08:48 AM

LC worked well for "blue Helix" ..i think its just that pan myc is more sensitive to the presence of bacteria and does not recover from mixing n shaking...If your stems are meaty enough to get a clean interior section then both ways should work..I prefer the popcorn idea though as you can more easily see whats happening ...Then go grain to grain for expansion if ness....gluk..

#5 spacecake

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Posted 17 September 2006 - 09:26 AM

Okay Golly ..I have put two pieces of a stem in peroxide for a short wile.
Then I cleaned it off, with distilled water very well...
I couldn't take the inside of the stem,because it was to small.

There I now in the glas along with the corn as you can see in the picture below.
If everything goes well,then I use this jar for spawning horse poo,and maybe take a couple of colonized corn pieces out, for further experiments !

Attached Thumbnails

  • Pan Florida clone stem 2int.jpg
  • Pan Florida clone stem1int.jpg


#6 golly

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Posted 17 September 2006 - 10:24 AM

Looks a bit wet Space...If your using just 3% H2O2 then there is a rather high probability of bacterial contam when taking an entire stem cross section...
If you have some more Pans to clone i would attempt to extract inner tissue with a pair of tweezers -just a tiny piece is all thats needed...

#7 spacecake

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Posted 17 September 2006 - 11:08 AM

I have the Viets on there way,..I'll try it with them..there are a little thicker right ?

I don't care if this one failes,just a quick experiment to fill my time...

#8 Hippie3

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Posted 17 September 2006 - 11:26 AM

good luck!

#9 golly

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Posted 17 September 2006 - 11:30 AM

Yeah,the stems of Vietnamese are easily thick enough to split and extract.
The clone will take about 4 days to show new mycelial growth - they usually turn black first and look like they're dead B4 sprouting...
I've lost my fair share o clones which hopefully means - i've learned alot lol....Glukkkkk

#10 reverend trips

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Posted 17 September 2006 - 11:33 AM

Man, it would be hard to rip them small stems open and get some inner tissue. Some kind of precision biopsy tool would be nice.
Hope it works out for ya spacecake!

#11 Hippie3

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Posted 17 September 2006 - 11:34 AM

chop a cross section then use a sterile needle to separate the strands

#12 BuckarooBanzai

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Posted 17 September 2006 - 12:15 PM

Never tried something as small as one of those, but with the bigger guys, letting them dry a little makes the tissue inside the stem shrink away from the exterior and become better differentiated and easier to remove.

I have to say, I've never seen one of these guys in person. Just how small are the stems? From the popcorn jars, they look very small, about 1/3 the diameter of a pencil.

Also, a small spoonful of dry verm will soak up any extra moisture on your popcorn.

Cool thread. Good luck!!!

#13 golly

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Posted 17 September 2006 - 12:53 PM

Viets and Goliath have stems of 3mm+ on the best of em, which are splitable ...Seven says he peeled the "skin" off the cap on the smaller ones to get a chunk of meat but said it was a PITA...

#14 alpiner

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Posted 17 September 2006 - 01:03 PM

at least that way you can clean it up if need be
is there a specific reasoning that you want to go directly to grain?

#15 Lazlo

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Posted 17 September 2006 - 01:42 PM

You don't have to use stem sections. You can always cut a sliver from inside a split cap.

#16 spacecake

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Posted 17 September 2006 - 03:39 PM

at least that way you can clean it up if need be
is there a specific reasoning that you want to go directly to grain?


I like speed and simplicity !

And thanks everyone,you all have some simple and good idea's !

BuckarooBanzai..that verm. thing is a good idea too,but I don't think I'll do that this time,..looks wet and it is a little bit,but don't forget it is a close-up picture !

#17 eu_por_exemplo

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Posted 17 September 2006 - 06:34 PM

Great Space!

Excelent job :greenboun

#18 spacecake

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Posted 19 September 2006 - 12:13 PM

Just a few minutes ago I went to have a look at my corn/clone jar...

I didn't expect to see anything yet,but was very suprized to see signs of growth on all two mushroom stems ! (picture)
Will keep you all updated on the speed of growth !

Attached Thumbnails

  • Pan clone 1 two daysint.jpg
  • Pan clone 2 two daysint.jpg


#19 dial8

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Posted 19 September 2006 - 01:31 PM

Very very cool!

So why did you decide on stem tissue wrather than some from the cap?

#20 spacecake

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Posted 19 September 2006 - 01:40 PM

Well,..I do the same things with cubies,so why not ?

Caps may hold some spore that would get in the 'way' and germinate not ?
Do you think cloning a cap is better Dail8..,or just less difficult ?




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