Cloning Pan Florida question
Posted 19 September 2006 - 01:57 PM
Yeah, I would not try to clone a section of the gills that was loaded with spores. That would be basically the same thing as a multi spore grow.
Posted 19 September 2006 - 04:38 PM
Posted 20 September 2006 - 12:03 AM
Off to a bang..!..How long did they take to show...?
After two days I saw sign of new growth on the stems Golly !
Posted 20 September 2006 - 06:53 AM
may the force be with u...heh....
Posted 20 September 2006 - 11:29 PM
As anybody who has tried, getting inner tissue from Pans, cap or stem it's a bitch not that it cant be done.
I therefore worked with stem sections splitting them down the center thinking to expose more inner tissue. This was dunked in 3% h2o2 for one minute and transfered to small baby food jars of sterilised millet.
I chose millet because it could be quickly prepared. It also provides many points of contact with the cloning tissue. I could have chosen WBS but having a single grain would be more specific.You'll see why I mention this in a minute.
Fourteen jars and two pan species were involved.
Additionally another six jars of an agar substitute comprised of BRF and water were prepared.
The result for all 14 jars was healthy myc growth on the grain with no signs of contamination on same.
The 6 jars on the other hand were a total loss. All sucumbed to bacterial contamination.
What I surmise from this at this point is that 3% h202 is insufficient to totally sterilise the tissue. The reason that the 14 grain jars did not contaminate was because of less points of contact with the tissue and because of more speceficity.
A small amount of collonised grain away from the transfered stem section was innoculated onto my favorite Pan spawn (Popcorn).
All looks good so this isn't a bad way of working. :D
Posted 21 September 2006 - 07:10 AM
Posted 23 September 2006 - 07:31 PM
Yesterday I saw myc. growth on different spots..don't now why ?
Hopefully it aren't spores germinating (can't be ?!)
Or myc network is growing inside out ,..so I can't see.
Whatever it is,..things seem to go wel !
Posted 23 September 2006 - 08:26 PM
Posted 24 September 2006 - 08:13 AM
Posted 24 September 2006 - 08:56 AM
Posted 24 September 2006 - 09:26 AM
i've always thought that it could be some microscopic fragments break off during a shake..
nice to see some good growth in those jars space
can't wait to see those clones fruited
Posted 24 September 2006 - 12:32 PM
I'll keep posting !
Posted 10 October 2006 - 09:49 PM
Totally agree Golly. In using the technique described every effort should be made to ensure the clone jars are not overly wet and therefore favor bacterial growth. Better on the dry side.
Interesting,,,U sometimes see that same effect in normal sterilized grain jars, where most do fine but one thats a bit wetter or less ventilated will slightly favour bacterial growth...Keep us posted Mycot ,thanx...
With overly wet grain,at best one gets very slow mycelial growth, at worst loss of the jar to contam.
Posted 10 October 2006 - 10:13 PM
Now, to continue this 'simplicity' idea.... Yur pretty advanced, Space! You ought to give agar a try before throwing it into the 'complicated' category! You have the skills and it isn't hard! Plus you wouldn't have to worry about those darn contaminated spores, if ya know what I mean!
Posted 10 October 2006 - 10:38 PM
Posted 11 October 2006 - 12:45 PM
All went very good and the clones are now spawned/cased and in the incubator, I think I will see some shrooms in two days or so !
I have some Viets pinning at this moment too,no big flush but very,very healthy shrooms.. with the double layer casing !
Pictures soon !
Posted 11 October 2006 - 01:26 PM
BH...I finally broke down and bought a magnetic stirrer for some edible cultures ...Was inspired by your last gro-log...Not ready to thankyou yet though as i havn't actually started...:lol:
Posted 11 October 2006 - 01:35 PM
Glad it's going well for ya Space...What method did u use for transfering your clone myc to spawn jars...?
Methode ?..lol !, just quickly dumped the stem in the jar in open air on the kitchen table and shaked it very rough !
I heard Seven saying that his Florida strain doesn't do wel when cloning.. so I didn't bother to do things carefully !
And I find it more fun when it works this way !