Paradox
©
Fisana

Jump to content


Photo
- - - - -

Roosters sham attempt at running spawn


  • Please log in to reply
47 replies to this topic

#21 FunG

FunG

    Been there, read that.

  • Free Member
  • 866 posts

Posted 11 December 2019 - 03:53 PM

No luck flashingrooster, my PC is leaking pressure somewhere and I've tryed everything to get rye/wbs to work to no prevail..it absolutely has to be at 15psi for 90minutes in order for grains to work out, I tryed pc'ing them twice and then shaking the jars to redistribute center kernels to the outer portion of the jar and then pc'ing again....nope, failed too.

This is why I cant wait for income tax season so I can go buy a all American sterilizer which are gasket less and above average of a house hold appliance, they're lab grade pressure cookers, these cheap kitchen ware ones suck!
  • flashingrooster likes this

#22 flashingrooster

flashingrooster

    Semi-Pro Taco Robot

  • Black VIP
  • 1,549 posts

Awards Bar:

Posted 12 December 2019 - 08:42 AM

I thought I read that you can just increase sterilization times to makeup for the small loss of pressure. It really only accounts for a a couple of degree temperature difference. It would be unfortunate if small temperature difference was the cause. 

 

Looks Like I am going to try the brf jars. As well as perhaps a few half pint of pint sized grain jars just for practice....  I have a big tote of grain to mess with so no to worried about waste. If my liquid culture turns out okay then there should be plenty of solution to go around



#23 FunG

FunG

    Been there, read that.

  • Free Member
  • 866 posts

Posted 12 December 2019 - 10:13 AM

If it's not at 15psi then there wont be enough heat to sterilize properly. The temperature needs to meet or exceed a certain degree in order for it to be effect (I cant remember what 15psi is in temperature) but even extending the time will not matter if it's not under xxx.xF

Trust me, I've been trying relentlessly to get wbs/rye to colonize without a hitch to no prevail, theres no solution other then to get the pressure cooker up to 15psi and maintain it for 90 minutes....

This is why all American sterilizes are recommended above all brands, no pressure leaks and no gaskets....I've gone threw two pcs a 23qt mirror and now this 23qt t-fal.....the t-fail otherwise known as t-fal worked once for wbs and never again which has me wondering cause the weight didnt rock at all during that PC cycle either.... ah well, I repeat my message I'm driving home, if it doesnt reach 15psi then it's not possible, not even fractional sterilization like I tried.

#24 flashingrooster

flashingrooster

    Semi-Pro Taco Robot

  • Black VIP
  • 1,549 posts

Awards Bar:

Posted 12 December 2019 - 12:47 PM

I think this was the thread I read

 

https://mycotopia.ne...-other-options/



#25 RutgerHauer

RutgerHauer

    Knows Nothing

  • Free Member
  • 1,009 posts

Awards Bar:

Posted 12 December 2019 - 01:12 PM

My PC goes to 1 bar, 14.5 PSI , It's the European standard, I think it differs 1 or 2 degrees Celsius.. I was a bit worried that it wouldn't work when I bought it - but seeing that there were no other options here other than importing and stealing from my own wallet.. It does the job for me, I'm glad it does. I sterilize for 1 hour and 45 minutes.

 

I wasn't aware that altitude influences the pressure.. I am lucky to live in one of the lowest parts in the word then.


Edited by RutgerHauer, 12 December 2019 - 01:22 PM.


#26 flashingrooster

flashingrooster

    Semi-Pro Taco Robot

  • Black VIP
  • 1,549 posts

Awards Bar:

Posted 12 December 2019 - 04:25 PM

What size jars do you use rutger?



#27 flashingrooster

flashingrooster

    Semi-Pro Taco Robot

  • Black VIP
  • 1,549 posts

Awards Bar:

Posted 12 December 2019 - 04:27 PM

Ya it's gotta be in Merica to be a full 15psi. For some reason amazon does not want to ship anything of that nature up here. 



#28 flashingrooster

flashingrooster

    Semi-Pro Taco Robot

  • Black VIP
  • 1,549 posts

Awards Bar:

Posted 12 December 2019 - 04:42 PM

Oh shit i am talking in two different threads...



#29 flashingrooster

flashingrooster

    Semi-Pro Taco Robot

  • Black VIP
  • 1,549 posts

Awards Bar:

Posted 15 January 2020 - 01:15 PM

So I was lucky enough to secure a syringe of some lC as well as a couple new spore syringe's

 

My first attempt at LC was a failure. I thought it would be a cheap way to get some practice with the hobby but what do i know

 

I have a few questions about LC 

 

Does liqiud culture go bad fairly quickly?  If so how long after initial colonization should one be placing it in the fridge, and then how long can you realistically expect to last?

 

And then how can you tell when a jar is ready to be used?



#30 sandman

sandman

    BACTERIA!

  • Honorary Former Staff
  • 3,814 posts

Awards Bar:

Posted 16 January 2020 - 09:35 AM

LC should keep in room temp for at least a month. If it's clean it will just grow to a certain point and become dormant. Eventually it will grow a thick mat on top and become too much of a pain to draw it into a syringe. I put them in the fridge after a few weeks if I'm not going to use it soon. Should last 6+ months or more in the fridge.

 

And a grains absolutely do not have to be cooked at 15 psi precisely to be sterilized. There are people in Thailand sterilizing in 55 gallon drums over a fire with plastic tarps for a lid. You just have to compensate with time. A poor craftsman blames his tools.


Edited by sandman, 16 January 2020 - 09:36 AM.

  • flashingrooster and Stencill86 like this

#31 flashingrooster

flashingrooster

    Semi-Pro Taco Robot

  • Black VIP
  • 1,549 posts

Awards Bar:

Posted 16 January 2020 - 06:59 PM

Perfect thanks, once i finish construction of my clean room it will be time to get back into a spawn run attempt

 

The grow kit i got in the mail made it seem so easy. Now I understand when they said we do all the hard work for you and send you good clean spawn


Edited by flashingrooster, 16 January 2020 - 07:03 PM.


#32 FunG

FunG

    Been there, read that.

  • Free Member
  • 866 posts

Posted 16 January 2020 - 07:05 PM

Make sure you properly cap the valve outlet with the regulator! *facepalm* only took me a year to figure out I had it on right the first time....


Gots myself 10L of wbs recovering from g2g :) ohhhhh yea!

#33 flashingrooster

flashingrooster

    Semi-Pro Taco Robot

  • Black VIP
  • 1,549 posts

Awards Bar:

Posted 15 February 2020 - 06:50 PM

Here's to hoping the clean room build was a success, It's essentially a space under the stairs lined with plastic for vapor barrier and easy cleaning

 

Looks like the brf jars are working. I shot up three with spore syringe and one with liquid culture. Running at room temp it took the GT liquid around ten days to show signs of growth. The spore syringe of Ecuador took three weeks to finally poke through to the outside of the jars. As well my two liquids look completely different from my failed attempt to make them from spore syringe. I used the same GT LC syringe to make two jars of lc. It feels good to finally see some growth after a couple complete failures of growth. I seemed to be restricted to a tiny bit of white that would easily get overtaken by contams

 

Can the wet brown rice give the appearance of contamination? I am a little unsure about some of the brown spots. They do not appear to grow, I would even say they might have shrunk. As well the mycelluim is increasing growth rate seemingly unhindered. No rotten smell of any kind. I think I have a good nose for that after my last few failures. Is there any harm in just letting them finish if there is concern about contamination?

Attached Thumbnails

  • 20200208_163422.jpg
  • 20200210_152943.jpg
  • 20200211_123448.jpg
  • 20200215_162621.jpg
  • 20200215_162641.jpg
  • 20200215_162700.jpg

  • Moonless, Choices and YoshiTrainer like this

#34 flashingrooster

flashingrooster

    Semi-Pro Taco Robot

  • Black VIP
  • 1,549 posts

Awards Bar:

Posted 23 March 2020 - 06:15 PM

My first cubes from syringe

Attached Thumbnails

  • 20200321_124037.jpg
  • 20200322_120450.jpg
  • 20200220_132748.jpg
  • 20200322_120624.jpg
  • 20200323_162703.jpg
  • 20200322_120822.jpg

Edited by flashingrooster, 23 March 2020 - 06:19 PM.

  • Arathu, ElPirana, Moonless and 2 others like this

#35 YoshiTrainer

YoshiTrainer

    Mycotopiate

  • Gold VIP
  • 494 posts

Awards Bar:

Posted 24 March 2020 - 10:01 AM

Nice job Rooster, congrats!
  • flashingrooster likes this

#36 flashingrooster

flashingrooster

    Semi-Pro Taco Robot

  • Black VIP
  • 1,549 posts

Awards Bar:

Posted 24 March 2020 - 04:01 PM

Not a big haul but it will do

 

I have seen the results from a bulk spawn on coir and verm from using grain spawn from a grow kit.  So I tried to skip brf jars and went to grain spawn. I went from zero success rate to more like sixty percent

 

Just happy with something finally growing off me injecting a syringe into it, there were a few failed grain attempts in bags and jars.



#37 flashingrooster

flashingrooster

    Semi-Pro Taco Robot

  • Black VIP
  • 1,549 posts

Awards Bar:

Posted 28 May 2020 - 03:56 PM

So it looks to me like my favorite filter for BRF is the poly fill. It's cheap, easy to find and replace. At first I was having some trouble finding those bag's of polyfil for a reasonable price. It hit me to just buy the 1.00 pillow from walmart when I couldn't find any polyfill bags there. There is a lifetime supply of poly in that pillow for the amount I use. I bought the wrong kind of micro pore tape, the plastic kind! It melted down in the pressure cooker leaving exposed holes when I took off my tinfoil. I had both kinds but for some reason went with the plastic type first.  :rolleyes:

 

I had not paid much attention when I bought those .22 micron syringe filters as the thread mentioning them was using them with liquid cultures. I had read they should be okay for grain somewhere so I gave it a shot, it was my first attempt with that setup and all my jars showed little to no growth. My second jar attempt I switched to polyfill and actually had 3/5 jars work for me. I am starting to wonder if it was the filters now after this third batch is turning out.  These photo's show all the failed jars just so happen to be the tight micron filters. Coincidence?

 

post-160704-0-41525000-1590699697.jpg

 

post-160704-0-43526300-1590699732.jpg

 

post-160704-0-23356600-1590699662.jpg

 

 

I had to take better photo's my first batch was a joke

 

20200528_145849.jpg 20200528_145840.jpg 20200528_145855.jpg


Edited by flashingrooster, 28 May 2020 - 04:13 PM.

  • Juthro likes this

#38 flashingrooster

flashingrooster

    Semi-Pro Taco Robot

  • Black VIP
  • 1,549 posts

Awards Bar:

Posted 28 May 2020 - 03:57 PM

I loosened the lids a tiny bit, probably fail but what the heck



#39 flashingrooster

flashingrooster

    Semi-Pro Taco Robot

  • Black VIP
  • 1,549 posts

Awards Bar:

Posted 28 May 2020 - 04:11 PM

Since I finally have some good looking spawn on the right  there I was hoping to spawn to bulk. I am going to try a ratio of 1:2 spawn to sub using coir and vermiculite. Should I be using equal parts coir and vermiculite?



#40 YoshiTrainer

YoshiTrainer

    Mycotopiate

  • Gold VIP
  • 494 posts

Awards Bar:

Posted 28 May 2020 - 05:36 PM

Nice Rooster, congrats on the healthy myc! I agree on the polyfil, works good at a great price!
  • flashingrooster likes this




Like Mycotopia? Become a member today!