I am hoping to get some feedback regarding my current progress.
I am going to be as detailed here as possible so please forgive the verbose post!
I am following Magic for the Masses almost exactly.
I got two syringes of GT from a reputable source. I am wondering if maybe one of them was mislabeled (see below).
I made my substrate in 3 batches (all in one session): 5 jars, 5 jars, then 2 jars.
I then sterilized the 12 jars in 4 batches (3 jars each in my Instant Pot - that is all that can fit).
I then inoculated all 12 jars with the 2 syringes (I used twice the amount of inoculant intentionally because this is my first grow).
I did not keep track of which jars were inoculated with which syringes (but I think it that it is becoming obvious).
The jars are stored in a cupboard that is opened a few times a day.
Temperature is about 69-71F and RH is about 40-45% (but I don't know if that matters right now).
Almost immediately 6 of the jars showed that the substrate at each inoculation point started to darken.
The other 6 jars did not darken.
This is what is making me think that the liquid in the two syringes was different.
The 6 jars that did not darken at the inoculation points started to colonize relatively quickly and 5 of them are doing quite well, while 1 is lagging (from what I have read that is normal).
The 6 jars that did darken colonized a little slower than the other batch, but seem to be growing well, with 5 of them pretty even with vigour, while 1 doesn't show any growth at all. That 1 also seems to have a bit of a discolouration within an inoculation point that may be bacteria? I can't smell anything, but I am just putting my nose to the inoculation hole.
Things are going well, but there are two things that are bugging me.
-I am almost positive that the two syringes had different liquid in them, since everything else was identical (lesson to myself to keep better records next time). I will email the vendor to get their take on it, but I wanted to get as much information as possible before I do that.
-Does the 1 uncolonized jar have some kind of bacteria in it?
If I could get some experienced feedback I would greatly appreciate it.
Thank you so much for any insight you may be able to provide!
And Happy New Year to everyone!!!
I have attached some photos for reference...
Photo 1: batch 1, what seems to be healthy colonization after 9 days
Photo 2: batch 2, what seems to be healthy colonization after 9 days
Photo 3: batch 2, jar with no colonization and maybe some bacteria in the middle of the darkened inoculation point?