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1st LC is this mycelium or a contam?


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#1 ChocolateStarfish

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Posted 20 January 2020 - 05:52 PM

I was extremely sterile and cleaned the hell out of everything, but i don't know.. Can anyone confirm or deny with any amount of certainty that this is or is not contaminated?:(51f098d0d72309a089298cc263870696.jpg

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18e3fed3cbf01086ef1ab19543828a61.jpg0b0dd621eb75d325a90ab6f662f387a4.jpg01681b21450adb14d9387910cf960622.jpg

Edited by ChocolateStarfish, 20 January 2020 - 08:51 PM.


#2 Randys67

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Posted 20 January 2020 - 06:08 PM

I was extremely sterile and cleaned the hell out of everything, but i don't know.. Can anyone confirm or deny with any amount of certainty that this is or is not contaminated?:(51f098d0d72309a089298cc263870696.jpg

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Hard to tell with liquid culture without using agar. Might just be "malt" and mycelium....

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#3 coorsmikey

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Posted 20 January 2020 - 07:37 PM

Cloudy usually is a for sure sign of contamination. Even malt starts out cloudy but the particulates settle to the bottom of the jar. The liquid in between the tufts of mycelium should be crystal clear. If the sugar carmalized the liquid may have an amber hue to but should be crystal clear. If you just shook the LC that may be an exception. I have learned over the years to eyeball a LC for cleanliness and unless you just gave that a shake I wouldn't trust that one to even test it.


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#4 ChocolateStarfish

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Posted 20 January 2020 - 08:47 PM

Cloudy usually is a for sure sign of contamination. Even malt starts out cloudy but the particulates settle to the bottom of the jar. The liquid in between the tufts of mycelium should be crystal clear. If the sugar carmalized the liquid may have an amber hue to but should be crystal clear. If you just shook the LC that may be an exception. I have learned over the years to eyeball a LC for cleanliness and unless you just gave that a shake I wouldn't trust that one to even test it.

I did just stir it with the magnetic stirrer. Heres a picture of it undisturbed275246a95466db734eee49dbc0715b80.jpg

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#5 onediadem

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Posted 20 January 2020 - 09:12 PM

Do up a test jar when in doubt.


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#6 coorsmikey

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Posted 20 January 2020 - 09:13 PM

Better, But for me to say 100% for sure that it is clean I still don't see the clarity that I am accustomed to seeing. It don't mean that it is contaminated though, just lacking the one thing that I have learned to tell for sure. If it is clean you will see what I'm talking about soon as more mycelium consumes the nutrients. It will go one way or the other and once you spot that clarity it will click and you will always know what to look for.


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#7 ChocolateStarfish

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Posted 20 January 2020 - 09:45 PM

Better, But for me to say 100% for sure that it is clean I still don't see the clarity that I am accustomed to seeing. It don't mean that it is contaminated though, just lacking the one thing that I have learned to tell for sure. If it is clean you will see what I'm talking about soon as more mycelium consumes the nutrients. It will go one way or the other and once you spot that clarity it will click and you will always know what to look for.

Thank you, i really like the straight forward advice. I really dislike the "you gotta find out for yourself" its like "well yeah i know that, but what CAN you tell me" lol . i really appreciate the comments, if anyone is used to seeing this lmk it is normal lol. Probably gonna make a small test jar as recommended. Thanks guys!

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#8 wharfrat

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Posted 15 February 2020 - 09:34 PM

moved topic to 'magic mushroom' forum



#9 Choices

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Posted 15 February 2020 - 09:53 PM

Hey man, I’m relatively new here but I had the same question when i did my first LC which was purchased from a vendor. Mine looked exactly like yours and I inoculated 3 cakes and unfortunately they were contaminated.

I since have made my own and coorsmikey i think is talking about this, in no way am I patting my own back I just wish someone showed me a non contaminated LC.

448f1c4b86991db5abb6b100569b8231.jpg

Edited by Choices, 15 February 2020 - 09:54 PM.


#10 macgyver

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Posted 15 February 2020 - 11:23 PM

Wouldn't trust it. Even if you used LME I don't like the tint. If you used water and honey and saw that tint it would be baaaaaaaad. I started using LCs, never had luck with LME which in my case started looking like that but never grew past it. It's hard to say with those pictures, but like OD said only way to know for sure is shoot up a jar.

 

I would try again. take it as you will my friend....

 

good luck!



#11 Fungi2b

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Posted 16 February 2020 - 12:59 AM

Put a lil to agar, then clean myc to a new l.c. whenever mine look like that they typically have a bacterial tam. Can you Smell anything around your filter?




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