In the waning years of the 20th century, a FOAF brought an unamed cubensis spore print back from Amsterdam. That coupled with an old, used PF TEK kit allowed for many wonderful camping trips and spiritual journeys. As time passed, life got in the way of living and spore prints were stored "for later". 10-12 years later, the spores ended up in my FOAF's, friend's, cousin's, plumber's, friend's possesion. Instead of using FOAFFCPF, I'm just going to use FOAF or he/him from now on.
After being stored in folded card stock taped closed in a dresser drawer, my FOAF prepared a spore syringe like usual but used the entire print. He used it to inject jars of PCed popcorn and was met with massive contamination! Liking a challenge, my friend dropped pieces of the cleanest popcorn kernels onto agar.
From that nasty agar, transfered a chunk to new agar, from that, a smaller chunk to new agar. From that, start over again because his box full of jars tipped over and the condensation apparently washed over the remaining contamination, spreading it again. After taking a scraping to new agar, then to new agar he was still fighting Cob Web at the very least.
After transferring a scraping to new agar, he added some H2O2 diluted to 5% to the old agar and swirled it around. This was a very bad idea!
The transfered agar grew out for a couple days again and then a scraping was transferred. The new old agar was then subjected to a 5 minutes soak of H2O2 diluted to 10%. After the H2O2 was dumped out all of the infected mycelium except it's very outer ring was cut out and removed. I think next time, he should do that in reverse, cut out then soak in H2O2. He also started over from a 2nd spore print and only scraped a tiny bit from the middle of the print, first transfers were done today.
The hope is to get rid of the contamination or knock it back so far that the mycelium can fight back or out grow it. At this point though, it is frustrating, he is stubborn and mad now, so the fight is on! He says it smells of cubensis mycelium, just soured so there is hope.
Any tips I can pass on to him would be helpful.
It would be incredible to see these mushrooms fruiting again after 10-12 years of storage!
1st pic spore print
2nd folded print
3rd corn on agar
4th 1st transfer
5th 2 transfers after starting over
6th week after 5% H2O2
Edited by YoshiTrainer, 11 February 2020 - 07:13 PM.