Or you can make a spore syringe, test it on a sterilized grain jar, if all works well and no contamination then the syringe can be used to mass innoc and depending on the spore load in the syringe, the clean spore solution can be expanded on by diluting with sterilized water allowing for 10cc to become 100 and if the syringe is clean then all you have to do is label each new set of jars 1,2,3 everytime you draw in new water that way if a problem arises you'll know at what point the cutt of is and know which to monitor (just made a long rec even longer ) ....dont ever mass innoc first! Also if the syringe is clean you can keep the tester jar and use it for a propigation process known as grain to grain. (Read about it)
That's how I roll....but assuming the syringe is dirty and its you're only source of spores then yea, agar would be required to clean up a healthy culture from the dirty syringe.
Trying to save you a step....just please remember the golden rule, never ever mass innoc prior to testing and you'll be rocking out Ms grows in no time. Agar requires a glovebox/flow hood which you probably dont have but the GB(glovebox) is cheaper then a flow hood to construct, there are some mighty good tutorials for their step by step assembly on mycotopia and internet in general so If you wish to go the agar route then that's what you'll need to do first.
Sponsor syringes are usually 95% of the time clean, I've ordered from various vendors about a dozen times and only ever received one bad batch of spore syringes and it was after someone else took over the buisness ;)
So yea, whichever way you find easier....its your decision. But learning agar is always the end result for genetic isolation and cloning for canopy flushes and what not...its advanced but simple shit.
Ans no, I havnt been drinking while posting! Not me sir's , ma'ama and both's!
Thanks for the tips Alpoehi and FunG! I was quite busy last few days so just got a chance to read this.
I have spore prints which I got from FSRE. I need to process those in syringes.
Then, I got some Vermiculite and Coco Coir. My question about those is if I should add whole grain rye or rye flour to the 2 before mentioned. Or should I stick with Coco and Vermiculite only.
Another skill to learn is sterilization with pressure cooker, which I don't have but might buy if sterilization is not possible with a regular cooking pot. Then again, I read about people doing all this with no sterilization.
After sterilizing rye / coco / vermiculite, I read I should drop spores from the syringes on rye or coco, but not vermiculite. Vermiculite is only for keeping the humidity levels in check from what I read and is mixed in later.
Finally, how long can I keep the spores in the syringe before they go bad? I heard prints are good for a few years, but didn't see any mention of how long can they be in liquid form in syringes.
I have also ordered a heating mat to help with the temperature in the first part of the process when mycelium is being formed. After the cake is all white, I can remove them from the heating mat, having in mind my room temperature is over 22 which it always is. And never more than 25. I'm talking in Celsius.
Off-topic, but an update, the earthquake was not as scary as it looks on pictures / videos. There was not so much damage and only the oldest buildings had damaged roofs or facade. If facade is the right word for it xD. Unfortunately, 1 child died which made it quite an event. Additionally, everyone left their homes, went outside, grouped up and talked with each other waiting for the situation to calm down: but that was even more unfortunate happening as we are now seeing a rise in people infected with the most recent corona virus...
Personally I am not scared about the virus and think I should get infected in order to boost my immunity and enrich my gene pool. Because, myself and a few friends, one of them being a PhD in Biology keeps telling us we are doing a big mistake evading this virus with the quarantine measures and not gaining the immunity from it, because if another, stronger one comes in 10 years or so, we are all going down with absolutely no chance of fighting back.
Edited by Shayazat, 05 April 2020 - 05:38 PM.