Can someone recommend an LC Tek. It was recommended i make a liquid culture, and I want to. I just need a Tek on the LC and also on how to make lids, though it's pretty self explanatory- a tek would help. Hopefully one with pics?
Also. Are home made self healing injector ports made of silicone gasket material functional enough for myco purposes? I can't find it now, but saw a how to on "how to make lids" and it showed this method.
Do I absolutely need a syringe filter and injector port ? I hate buying stuff from Amazon unless I absolutely have to. I used to use Tyvek Fed Ex Envelopes for lids. Just used silicone after inoculation. Is this something anyone still does? It always worked well.

Finding a LC Tek: Making lids
#1
Posted 27 May 2020 - 07:52 PM
#2
Posted 28 May 2020 - 06:08 PM
For injections ports I’ve only used RTV gasket maker, I’ve heard someone use silicone before though . I don’t have a syringe filter on mine either . I use quite a bit of tyvek but I remember reading it was discouraged for LC (ive done it tho lol) .
I saw someone take a lid Andrew put two holes in it one was made into a SHIP and the other had a small brass fitting you’d find in the plumbing section stuffed with poly fill inside thr brass. Put that in the lids second hole and use gasket sealer where it meets up with the lid on the inside And out.
- Zorkloin likes this
#3
Posted 28 May 2020 - 09:24 PM
Thanks, I could not find any TEKs for the life of me
- Sidestreet and Mycol like this
#4
Posted 29 May 2020 - 05:25 AM
There are instructions for my favorite lids on page 58 of the "Magic for the Masses" PDF linked in my signature.
Also here's a good one: https://mycotopia.ne...e-test-of-time/
Found here: https://mycotopia.ne...st-of-the-best/
Edited by Sidestreet, 29 May 2020 - 05:31 AM.
- Wonky1 likes this
#5
Posted 15 June 2020 - 08:55 PM
I bought some .03micron syringe filters, and also some self healing injector ports. I'm deciphering a lot of info. Are the
syringe filters for gas exchange? I'm seeing several kinds of lid designs
I can't understand how the long gauge needle we use in myco would not just put a hole in those syr. filters
#6
Posted 15 June 2020 - 09:43 PM
500ml water, 5g MEA, 1Tbl Karo, PC 30 min @ 15psi.
#7
Posted 16 June 2020 - 12:42 PM
Just was confused on purpose of syringe filter, gas exchange only? I have some and would rather use them than post office Tyvek
#8
Posted 16 June 2020 - 01:21 PM
#9
Posted 16 June 2020 - 07:11 PM
I'm assuming one must use the syringe filter with no needle ?The layout of most lids I see have a pre made or silocone made self healing port, with a syringe filter on the other side.
Why both? edit: Oh I see what you're saying about not making a vacuum, that's the purpose of having both?
Oh and I can't find the SHIP acronym, something entry point? Sorry , I'm stressed about this. Cramming my brain waiting for pins
Edited by Zorkloin, 16 June 2020 - 07:15 PM.
#10
Posted 18 June 2020 - 08:26 AM
Has anyone had trouble with the cheaper GREY injection ports? I was reading that the red ones are more reliable, and that the grey ones don't hold up?
Those of you who have done it.. Is making a port out of aquarium silicone just as reliable?
#11
Posted 09 August 2020 - 04:35 AM
- Mycol likes this
#12
Posted 01 September 2020 - 12:17 PM
The syringe filter becomes a permanent SHIP for your LC jar, allowing you to draw up your LC w/out creating a vacuum. Probably some gas exchange too.
@Yosh, I keep seeing these permanent filters on jars. I get the use when using them to innoculate/incubate substrate. I am confused about using them on a LC, don't you run risk of the filter getting wet from the liquid culture?
#13
Posted 01 September 2020 - 01:52 PM
The syringe filter becomes a permanent SHIP for your LC jar, allowing you to draw up your LC w/out creating a vacuum. Probably some gas exchange too.
@Yosh, I keep seeing these permanent filters on jars. I get the use when using them to innoculate/incubate substrate. I am confused about using them on a LC, don't you run risk of the filter getting wet from the liquid culture?
the method he is describing in which you use a syringe with the plunger removed and stuffed with polyfill does not present an opportunity for the LC to touch the filter material. All that is entering the jar is the needle. Most jar lids designed for LC use are designed with an air exchange valve that will not be impacted by liquid hitting it... The jar lids that have the filter exposed are typically used for spawn.
- YoshiTrainer likes this
#15
Posted 01 September 2020 - 02:08 PM
Zorkloin, I'm noticing my grey injection ports are showing wear from repeated use. Might look into red ones next time I order?
- NardDogOverdrive likes this
#16
Posted 01 September 2020 - 07:32 PM
Narddog, the filter material is hydrophobic so no worries about LC saturating it.
Zorkloin, I'm noticing my grey injection ports are showing wear from repeated use. Might look into red ones next time I order?
I’ve Been noticing that my self. But have not researched any alternative. What are the red ones you speak of Yoshi? Heavier duty SHIP?
- YoshiTrainer likes this
#17
Posted 01 September 2020 - 11:18 PM
#18
Posted 02 September 2020 - 12:20 AM
Back far enough.
#19
Posted 02 September 2020 - 03:00 AM
The syringe filter becomes a permanent SHIP for your LC jar, allowing you to draw up your LC w/out creating a vacuum. Probably some gas exchange too.
Reading back through this, I goofed. SHIP is the rubber stopper, syringe filter is what keeps a vacuum from forming as you draw up your LC.
#20
Posted 02 September 2020 - 05:37 PM
Wait could you even successfully extract any mycelium through syringe filter?
I did not care for them as fitlers when used on either grain or brf. The .22 micron one's i bought did not seem to provide enough gas exchange, all the jars that were inoculated with those .22 filters stalled out. I thought it was some proof when I loosened the lids and they came out of the stall. I wonder if anyone else has had the same experience or I just failed at something else
They seem great for liquid cultures though