20 replies to this topic

[Zorkloin]

Can someone recommend an LC Tek. It was recommended i make a liquid culture, and I want to. I just need a Tek on the LC and also on how to make lids, though it's pretty self explanatory- a tek would help. Hopefully one with pics?

Also. Are home made self healing injector ports made of silicone gasket material functional enough for myco purposes? I can't find it now, but saw a how to on "how to make lids" and it showed this method.

Do I absolutely need a syringe filter and injector port ? I hate buying stuff from Amazon unless I absolutely have to. I used to use Tyvek Fed Ex Envelopes for lids. Just used silicone after inoculation. Is this something anyone still does? It always worked well.

[Mycol]

If you scroll to the bottom of the page in the right hand corner you’ll see “the new vaults” in there you will see some lc teks.

For injections ports I’ve only used RTV gasket maker, I’ve heard someone use silicone before though . I don’t have a syringe filter on mine either . I use quite a bit of tyvek but I remember reading it was discouraged for LC (ive done it tho lol) .

I saw someone take a lid Andrew put two holes in it one was made into a SHIP and the other had a small brass fitting you’d find in the plumbing section stuffed with poly fill inside thr brass. Put that in the lids second hole and use gasket sealer where it meets up with the lid on the inside And out.

[Zorkloin]

Thanks, I could not find any TEKs for the life of me

[Sidestreet]

There are instructions for my favorite lids on page 58 of the "Magic for the Masses" PDF linked in my signature. 

 

Also here's a good one: https://mycotopia.ne...e-test-of-time/

 

Found here:  https://mycotopia.ne...st-of-the-best/

Edited by Sidestreet, 29 May 2020 - 05:31 AM.

[Zorkloin]

I bought some .03micron syringe filters, and also some self healing injector ports. I'm deciphering a lot of info. Are the
syringe filters for gas exchange? I'm seeing several kinds of lid designs

I can't understand how the long gauge needle we use in myco would not just put a hole in those syr. filters

[YoshiTrainer]

For LC I use syringe filter and resealable port. Drill your holes in the lid tight. The resealable port is pressed in place, the syringe filter is also pressed in place with a little high temp hot glue to make seal between the filter and lid. Remember to scuff the surface of the lid before gluing. I also use a silicone O ring inside the lid and a few broken bit's of glass in the jar.

500ml water, 5g MEA, 1Tbl Karo, PC 30 min @ 15psi.

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[Zorkloin]

Just was confused on purpose of syringe filter, gas exchange only? I have some and would rather use them than post office Tyvek

[YoshiTrainer]

The syringe filter becomes a permanent SHIP for your LC jar, allowing you to draw up your LC w/out creating a vacuum. Probably some gas exchange too.

[Zorkloin]

I'm assuming one must use the syringe filter with no needle ?The layout of most lids I see have a pre made or silocone made self healing port, with a syringe filter on the other side.

Why both? edit: Oh I see what you're saying about not making a vacuum, that's the purpose of having both?

Oh and I can't find the SHIP acronym, something entry point? Sorry , I'm stressed about this. Cramming my brain waiting for pins

Edited by Zorkloin, 16 June 2020 - 07:15 PM.

[Zorkloin]

Has anyone had trouble with the cheaper GREY injection ports? I was reading that the red ones are more reliable, and that the grey ones don't hold up?

Those of you who have done it.. Is making a port out of aquarium silicone just as reliable?

[Enigkotik]

No needle. The filter allows for withdrawing culture and air exchange. SHIP stands for self-healing injection port, which you can also use to withdraw the culture. I purchased gray 20 mm ports from Amazon, and they work great. I affixed them with E6000 glue. Just make sure the ones you buy can withstand being pressure cooked. Don't forget to add the magnetic stir bar in there, and if you have the money get a magnetic stirrer to go with it. The one I purchased was about $30, and it works great. It makes it super easy.

[NardDogOverdrive]

The syringe filter becomes a permanent SHIP for your LC jar, allowing you to draw up your LC w/out creating a vacuum. Probably some gas exchange too.

@Yosh, I keep seeing these permanent filters on jars.  I get the use when using them to innoculate/incubate substrate.  I am confused about using them on a LC, don't you run risk of the filter getting wet from the liquid culture?

[PJammer24]

 

The syringe filter becomes a permanent SHIP for your LC jar, allowing you to draw up your LC w/out creating a vacuum. Probably some gas exchange too.

@Yosh, I keep seeing these permanent filters on jars.  I get the use when using them to innoculate/incubate substrate.  I am confused about using them on a LC, don't you run risk of the filter getting wet from the liquid culture?

 

 

the method he is describing in which you use a syringe with the plunger removed and stuffed with polyfill does not present an opportunity for the LC to touch the filter material. All that is entering the jar is the needle. Most jar lids designed for LC use are designed with an air exchange valve that will not be impacted by liquid hitting it... The jar lids that have the filter exposed are typically used for spawn.

[PJammer24]

 

 

These lids with this style valve for air exchange and a SHIP (self healing injection port) are well suited to LCs

[YoshiTrainer]

Narddog, the filter material is hydrophobic so no worries about LC saturating it.

Zorkloin, I'm noticing my grey injection ports are showing wear from repeated use. Might look into red ones next time I order?

[Choices]

Narddog, the filter material is hydrophobic so no worries about LC saturating it.

Zorkloin, I'm noticing my grey injection ports are showing wear from repeated use. Might look into red ones next time I order?

I’ve Been noticing that my self. But have not researched any alternative. What are the red ones you speak of Yoshi? Heavier duty SHIP?

[YoshiTrainer]

Sorry Choices, Zorkloin mentioned red ports first. I still have a few grey ones and planned on looking into red or other new ones when the time comes.

[Choices]

Ahhh didn’t read
Back far enough.

[YoshiTrainer]

The syringe filter becomes a permanent SHIP for your LC jar, allowing you to draw up your LC w/out creating a vacuum. Probably some gas exchange too.

Reading back through this, I goofed. SHIP is the rubber stopper, syringe filter is what keeps a vacuum from forming as you draw up your LC.

[FLASHINGROOSTER]

Wait could you even successfully extract any mycelium through syringe filter?

 

 I did not care for them as fitlers when used on either grain or brf. The .22 micron one's i bought did not seem to provide enough gas exchange, all the jars that were inoculated with those .22 filters stalled out. I thought it was some proof when I loosened the lids and they came out of the stall. I wonder if anyone else has had the same experience or I just failed at something else

 

 

They seem great for liquid cultures though