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Blue Oyster Mushroom Grow Log


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#1 Auhron

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Posted 17 October 2020 - 04:07 PM

This will be my first attempt growing mushrooms. I'm going to try to keep a reasonable log posted on steps I have taken.

 

I started out by getting some mushrooms from the grocery store. I tried to get a spore print, but the mushrooms were old looking and the gills were wet so I didn't get anything. I opted to swap them and attempt to get a culture that way.  OMG BAD IDEA. I had 3 petri dishes of molds, yeast and random bacterias, though all showed signs of mycelium growth.  I deemed 2 of the dishes unworthy to attempt salvage.  The third dish was marginally healthier (I use the term loosely). I cut two sections from that dish and transferred them. One of those samples did nothing in its new dish. It just sat there. The other however grew a fair bit.

There are two spots of contamination I need to transfer away from.

 

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Malt Extract Agar being incubated with no light @ 75F


Edited by Auhron, 17 October 2020 - 05:33 PM.

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#2 Auhron

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Posted 17 October 2020 - 04:25 PM

Also moderators... I'm srry but there is an obnoxiously large version of one of these pictures in the gallery... I was having fun figuring out why it wasn't uploading..... feel free to remove that one.... I'll verify they are a reasonable size before I upload in the future...

 

Nevermind, I figured out how to fix that image over in the gallery myself.  :biggrin:


Edited by Auhron, 17 October 2020 - 04:30 PM.


#3 Auhron

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Posted 21 October 2020 - 03:46 AM

Important Safety Notice:  When using alcohol to clean your work space (inside Still Air Box) wait for some time before attempting to flame sterilize any tools... Seriously. This is important... 

 

 

I took two transfers from my culture, away from the contamination onto new dishes. Both of those dishes promptly showed signs of contamination when I started incubation, the oyster mushroom mycelium is also growing out. Original sample still has only the pre-existing contamination and has almost completely filled the plate! The contam appears to be the same yeast that has been on all the plates I poured that batch (not in a SAB).  I prepared a new batch of plates inside of the SAB today and will be taking multiple transfers (probably 3 from each of the 3 plates) in the hopes of obtaining a clean sample. The new batch of agar plates is Activated Charcoal - Malt Extract Agar. 

 

The Liquid Culture started from a large chunk of interior stem has broken up a fair bit and is beginning to show signs of expansion in its mason jar. I also have a liquid culture of Shimeji that is showing nice signs of growth as well.

 

Pictures coming. =) 


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#4 bezevo

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Posted 22 October 2020 - 05:38 PM

cool  grow welcome



#5 Arathu

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Posted 23 October 2020 - 05:02 AM

Yes indeed using flammable substances for disinfecting things and open flame sterilization are mutually exclusive things......please be careful everyone.....

 

BUY A FIRE EXTINGUISHER and keep it close at hand when working.......

 

Welcome to Mycotopia Auhron.......I love some oyster mushrooms.. 

 

A


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#6 Auhron

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Posted 18 November 2020 - 09:38 PM

Worst log ever... I did some things...with the mycelium. It grew. BOOM!!

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Primordia?!?!!

I have The Block in fruiting conditions now. No promises but I'll try to take a picture each day.

So the Last month of grow log. I built a flow hood. Dual 99.97% HEPA Filtered (one smaller intake, and the larger 11.5" x 13.5" outflow). so only 1 Square foot of workspace, but I went from mad contamination open air transfers, to mid contam in a SAB... to fucking fireballs in shmuvboxes Safety is not overrated. Enter the not laminar flow Hood. My airflow output is not fast enough to truly be laminar, but it is close.

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I read a book...

Another round of transfers. I managed to get 2 clean agar dishes, first attempt with flow hood.
Both attempts to start a liquid culture from these agar plates were unsuccessful. I did manage to get a grain jar going however. I then transferred my grain (1/2pint) into supplemented sawdust substrate (2 Pints). Substrate comprised of Softwood Pellets (no chemicals), Soy Hull Pellets, and a tiny bit of Gypsum. This substrate was hydrated to field capacity with boiling water then sterilized for 2 hours at 12psi. I then expanded these two pint jars into a medium grow bag (5.25pints by volume or roughly 2.5lbs.). I placed my block in the fridge for 4 hours.
The growth observed in the photo was not present prior to the fruiting blocks time in the fridge.
The Block is now sitting in a cool space away from direct light, but not in the dark. A deep Seedling starting tray with humidity dome is being used. Fruiting Conditions will be my biggest challenge. I have a rather substandard humidifier working to raise the RH.

I will be starting additional grain jars and attempting to get a liquid culture going tomorrow. I will also expand the mycelium on the plate I use tomorrow to an additional 2 plates. I'm going to try to get it rolling where I'm constantly fruiting a block of something. Incubation space isn't a problem, an ample supply of jars and a pressure cooker. I have a bunch of lentils, I've read mixed reviews on using them for spawn. Considering trying them. a couple 1/2 pint jars just to see.

The Non-Oyster Projects:
I have a button mushroom LC. super aggressive, from spore, abandoned agar dish due to contamination.
I have Shimeji and Portobello LC's started from mushroom tissue. the LC's are super clean looking but the growth rate is horribly slow. No agar work was done for these.
I have a couple of failed attempts at LCs for both Oyster and Cremini. Cremini was cloned on MEA, lost both agar and LC to contamination.

I ran through my stack of 25 disposable petri dishes pretty quick before I built the flow hood. I got a couple dozen glass petri dishes, more expensive initially but if I don't break them it will prove to be less wasteful. Far easier to sanitize than plastic.

I have ordered two LC syringes. Lions Mane and Shiitake. I am looking forward to working with both as well as eating them.

Edited by Auhron, 19 November 2020 - 02:05 AM.


#7 Auhron

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Posted 20 November 2020 - 04:12 PM

36hrs later the primordia still had done nothing, there was no visible condensation anywhere so I decided that I needed a better humidifier. I replaced what I was using with this fogger:

 

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Pumping super humid air into the growing tray. 18 hours after setup the spot of primordia now resembles pinheads.

 

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sorry about the shit photos. My camera isn't great and I'm no photographer. 


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#8 Auhron

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Posted 20 November 2020 - 10:02 PM

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better photo. It is growing.



#9 Auhron

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Posted 21 November 2020 - 03:36 AM

Okay! Important Update... This is not what Blue Oyster Primordia / Pinheads is supposed to look like. From what I have seen elsewhere it's supposed to be blue...

 

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Better photos this time round... I have read that the tips of the primordia can start a tan color and then turn blue, but that doesn't seem to be what is happening here. The Mushroom I swabbed was BLUE! BLUE!!!


Edited by Auhron, 21 November 2020 - 03:43 AM.


#10 Auhron

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Posted 21 November 2020 - 11:01 AM

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#11 Auhron

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Posted 21 November 2020 - 06:33 PM

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Mystery Mushrooms! Not Blue.

I am torn between disappointment and excitement.



#12 Auhron

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Posted 21 November 2020 - 11:46 PM

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#13 jrh

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Posted 22 November 2020 - 05:59 AM

I have a bunch of lentils, I've read mixed reviews on using them for spawn. Considering trying them. a couple 1/2 pint jars just to see.&&0){for(var>)throw>

 

A little trick might be to add some verm to the bottom of the jar before adding your lentils. That would let you use more LC to inoculate.

 

I'll give the trick a try myself after I receive some SSIP to make some grain spawn lids. I have 3 pint tester jars (2 oats, one soybeans) doing not much, but they're using IV syringe filters which are probably good for LC, but seem less than ideal for other situations.


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#14 Auhron

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Posted 22 November 2020 - 10:23 AM

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#15 Arathu

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Posted 22 November 2020 - 10:43 AM

Absolutely beautiful! The cap is where it's at and it looks like someone will be enjoying fresh caps shortly....YUM!

 

Nice work!

 

A


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#16 Auhron

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Posted 22 November 2020 - 05:55 PM

I might try a tiny bit of verm in with the next batch of lentils, assuming I get any growth and use them again. Thanks jrh, I had read that tip previously but had forgotten.  Thanks Arathu! The Cluster is about 3 inches tall right now.

 

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Just a few short days away from enjoying these beauties.


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#17 Auhron

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Posted 23 November 2020 - 12:25 AM

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Edited by Auhron, 23 November 2020 - 12:26 AM.


#18 Auhron

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Posted 23 November 2020 - 01:39 PM

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Very long looking stems. I am going to increase the FAE within the chamber. They are getting bigger!


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#19 Auhron

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Posted 25 November 2020 - 12:30 AM

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So it was probably too late in the process for the increased FAE I gave them to really make a difference. I might be picking and weighing these beauties tomorrow night, though I have not decided for sure.

The edges of the caps are starting to turn outwards so I think they are close to mature.



#20 Auhron

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Posted 25 November 2020 - 10:34 PM

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Harvested, 6.85oz.  Going for second flush. Increasing the FAE from the start, hopefully there will be some level of improvement to the growth pattern. Less stem, more cap.


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