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#1 BrotherDekatessera

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Posted 26 October 2020 - 10:57 AM

these MSS dubtub experiments finally paid off. these three Costa Rica MSS colonized and started pinning in about 7 days

(read anywhere from 6 to 8 because my time awareness is retarded) 

gallery_167012_3_9664.jpg

 

gallery_167012_3_32577.jpg

 

gallery_167012_3_233677.jpg


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#2 BrotherDekatessera

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Posted 26 October 2020 - 11:20 AM

These Costa Rica bags from agar slurry LI are just about fully done, 4 days in.

This was the first "isolation" .. 

went from MSS to agar, transferred the first couple of spots to germinate from a few dishes, two of those grew out looking much like what most people would call a "monoculture" .. never seen such clean, uniform growth on a first transfer so I went to grain, i.e. these bags. as you can see, the Costa Rica First round, second selection is a boss.

 

gallery_167012_3_2307221.jpg

 

 

not doing bad at all for four days, but also not pushing as hard as the 1:2, the first round, first selection :

 

 

gallery_167012_3_2645230.png


Edited by BrotherDekatessera, 26 October 2020 - 11:30 AM.

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#3 BrotherDekatessera

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Posted 26 October 2020 - 11:27 AM

this PF Classic was a disaster.

 

MSS to 20 agar dishes. 14 contammed' which is about 13 more than my average rate.

 

5 did nothing.

 

transferred the only 4 healthy-ish cultures, 10 dishes, 5 contammed.

 

agar slurried the remaining 2 genetics (keeping a slice of course) and 1 of the bags contammed.

 

here is whats left. 

 

gallery_167012_3_494176.jpg

 

 

not bad for four days in, but looking like a retard so far next to the Costa Rica. maybe just an unlucky syringe. I dont know, since Ive never run PF classic before. 

 

is it usually this much of a pain in the ass??



#4 mushit

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Posted 26 October 2020 - 02:24 PM

Ya, it sounds like you had a dirty syringe.  One good reason to do many plates.  It gives you a better chance of getting at least some myc to work with.

Great looking grow so far though.

 

I haven't had the pleasure of getting a PF print yet, so I cannot give any advise on it other than.............. wait for it............A cube is a cube.  :tongue:


Edited by mushit, 26 October 2020 - 02:24 PM.


#5 coorsmikey

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Posted 26 October 2020 - 06:42 PM

What does the additional S in MSS mean? I get Multi-Spore but just trying to understand the additional one.



#6 ElPirana

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Posted 26 October 2020 - 06:50 PM

What does the additional S in MSS mean? I get Multi-Spore but just trying to understand the additional one.


I’m guessing MSS = multi spore syringe.

#7 coorsmikey

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Posted 26 October 2020 - 06:54 PM

Well then I have another proposal. MSSS = Multi Spore Solution Syringe.



#8 jrh

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Posted 26 October 2020 - 07:43 PM

OMG, MS is multispore syringe? I've searched up and down the forum trying to figure out what variety of cube or exotic MS was.  :tongue:


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#9 ElPirana

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Posted 26 October 2020 - 08:33 PM

OMG, MS is multispore syringe? I've searched up and down the forum trying to figure out what variety of cube or exotic MS was.  :tongue:

I hear ya! You know, after four years in the hobby, last month I just figured out what OMC stands for! lol

#10 BrotherDekatessera

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Posted 27 October 2020 - 08:06 AM

 wait for it............A cube is a cube.  :tongue:

truth



#11 BrotherDekatessera

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Posted 27 October 2020 - 08:17 AM

Well then I have another proposal. MSSS = Multi Spore Solution Syringe.

What about Multi-Spore Solution Syringe Spore Spreading System 

 

Everyone knows the bigger the acronym or abbreviation is the more scientifical-ish it is. if we can add enough S's we can finally call ourselves mycologists.



#12 BrotherDekatessera

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Posted 27 October 2020 - 11:19 AM

gallery_167012_3_81297.jpg


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#13 BrotherDekatessera

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Posted 29 October 2020 - 09:19 AM

Mandarin Cookies, no till living soil, greenhouse.

 

gallery_167012_3_319495.jpeg

 

same, 

gallery_167012_3_705829.png


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#14 BrotherDekatessera

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Posted 29 October 2020 - 09:20 AM

PF Classic pins

gallery_167012_3_168811.jpg



#15 BrotherDekatessera

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Posted 29 October 2020 - 09:21 AM

PCCR Pins

 

gallery_167012_3_584497.jpg






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