Paradox
©
Fisana

Jump to content


Photo
* * * * * 5 votes

Crossing strain varieties via swab to spore solution


  • Please log in to reply
377 replies to this topic

#321 sandman

sandman

    Saint PP

  • Honorary Former Staff
  • 4,133 posts

Awards Bar:

Posted 14 April 2021 - 09:17 PM

This is some next level thinking man I get it. You are clever.

 

So after you bust it through the foil sphincter on your quarantine section syringe body you dip the tissue in the jars of water to rinse it and then drop it in the grain jar right?

 

It's even with a complimentary sterile air environment. 


Edited by sandman, 14 April 2021 - 09:39 PM.

  • fahtster likes this

#322 fahtster

fahtster

    Oven bag specialist

  • OG VIP
  • 2,254 posts

Awards Bar:

Posted 14 April 2021 - 10:10 PM

Thanks man.. yeah you got it. I puncture the foil just enough to let the water drain out and the tissue stays inside the syringe barrel.. then break it all the way while it’s on its side and then tap it directly into the first jar of water... swirl it around with the tweezer.. tweez it into the 2nd jar.. swirl... then drop it into the grain jar... put the lid on and cut out of the bag. If it’s a clone I really really want, I’ll attach two syringe barrels and take two tissue sample and have two grain jars just in case.

Faht

Edited by fahtster, 14 April 2021 - 10:11 PM.

  • sandman and rockyfungus like this

#323 fahtster

fahtster

    Oven bag specialist

  • OG VIP
  • 2,254 posts

Awards Bar:

Posted 15 April 2021 - 08:47 PM

PE/pesa RADS cv/brf cakes (2) birthed and being dunked.. don’t know what to expect from these

2A932023-AF12-4230-B426-542BA8D1E384.jpeg

The PE/apeu aren’t far behind... waiting for the very bottoms to finish on these.. I’m gonna have to do some surgery on the right cake.. got a bit of bacteria in that one spot but should be too big of a deal.

D1659E54-5CA3-49B5-95EF-79EAB1DBA1C7.jpeg

The left one is pinning on the top and they’re really light pins but definitely (from what I can tell) aren’t albino so that’s promising.

Also did a grain spawned to cv 1/2 pint tester jar of each that should be pinning in a week or so

086DD5B5-34C7-4119-9228-8F062B05ECF4.jpeg

Faht

Edited by fahtster, 15 April 2021 - 08:51 PM.

  • ElPirana and YoshiTrainer like this

#324 rockyfungus

rockyfungus

    Mycotopiate

  • Free Member
  • 1,127 posts

Posted 15 April 2021 - 09:14 PM

Sorry but that looks so tedious to me compared to working in a SAB, but obviously that is way more sterile.

Keep up the killer work so amazing. 


  • fahtster likes this

#325 fahtster

fahtster

    Oven bag specialist

  • OG VIP
  • 2,254 posts

Awards Bar:

Posted 15 April 2021 - 10:07 PM

Yeah I know it looks tedious but it’s really not.. once you have all the tubes and syringe ports made, they’re totally reusable so you just grab and go. Setting up the foil on the quarantine section is the hardest part and that’s pretty easy. Like with my print bags, I attach a poly stuffed syringe barrel and inflate the bag with a fish tank bubbler and that makes a world of difference... tons of room to move things around in the bag. Trust me, I know how it looks lol. I’ve had bad experiences with SAB’s and sleeper contams ruining tub grows before the flush and if I have to build these things to ensure that doesn’t happen, I’m all for it.

Syringe stuffed tight with poly attached to a bubbler hose to inflate..
BF9195AD-F6C0-4D31-8452-9B15426C5C36.jpeg

Faht

Edited by fahtster, 16 April 2021 - 01:46 AM.

  • sandman and YoshiTrainer like this

#326 rockyfungus

rockyfungus

    Mycotopiate

  • Free Member
  • 1,127 posts

Posted 15 April 2021 - 11:02 PM

I always forget you don't use agar, that is just such awesome planning and a great workaround. I would close up the bag and forget my grain or something. You keep doing you, really amazing work. 

 

First transfers of "PEA+" are looking clean growing them out a bit further and will go straight to grain with the fastest looking plates. Some characteristics I've noticed from playing with PE on agar is that it's a slow colonizer (who would of thought that), it's more tomentose and thin compared to the average cube. AA+ is tomentose too but denser, not a quick colonizer either but seems a bit faster then PE.

 

This is agar doesn't mean shit with what will express I don't even know if I had any successful crosses.


  • fahtster likes this

#327 fahtster

fahtster

    Oven bag specialist

  • OG VIP
  • 2,254 posts

Awards Bar:

Posted 15 April 2021 - 11:15 PM

Thanks man.

Only one way to find out! Good luck yo! :)

Faht

#328 fahtster

fahtster

    Oven bag specialist

  • OG VIP
  • 2,254 posts

Awards Bar:

Posted 17 April 2021 - 12:15 PM

Faht: I have 2C and 3C round and rectangle. I'll have to try the straight water method as perlite with bleach always smells rancid in a few days. Basically I go from baby jar and spawn to a larger tupperware. I'll have to dig around for any larger (8C) containers. Been meaning to give PE even more headroom during spawn to try and double case right from jar...Thanks for all the fruiting method.

Here’s the “water tub” 1/2 pint tester FC..
17ACC1DE-8850-4D91-9D8B-D97944508AE0.jpeg

I like it.. nixing the perlite is great.. it’s so messy and stepping on it is one of my pet peeves lol. I’ve changed the water once (didn’t use bleach the second time); it’s been a few days since.. everything smells fine and it was pretty easy to change the water.. just took the jars out, replaced water with tap and put the jars back in. Ez pz. I can’t remember where I read it.. maybe fb, but someone suggested letting the top layer dry out for a day or two when you started seeing knots and then rehydrating. They said they see better pin sets that way. I did that and sure enough, I saw a bunch of pins the day after I rehydrated.

Those are all pea+.. the back right one is the f1 clone culture I just revived after 3 months and the rest are f3 ms. That front left one is pretty bangin. I think pea+ is gonna be a nice variety all said and done.

Faht

Edited by fahtster, 17 April 2021 - 12:21 PM.

  • sandman, YoshiTrainer and rockyfungus like this

#329 fahtster

fahtster

    Oven bag specialist

  • OG VIP
  • 2,254 posts

Awards Bar:

Posted 20 April 2021 - 11:03 PM

Here’s the water tub.. yesterday and today.. all the f3 jars are done.. just have the f1 clone to finish up
2FBE8DC7-9C47-4F3E-8587-BC4D9A012882.jpeg 233DC0DB-616F-49F4-82A6-F344F28B6E80.jpeg

Second flush of this PE/pfrs clone bag https://mycotopia.ne...1617851540.jpeg is coming in

D0548F67-B536-45F7-B885-09DE26B29363.jpeg 2E484BFC-F7A9-498C-83C9-62F8FF873C32.jpeg

Crazy how much different they look on the 2nd flush.. look more like the other suspected PE hybrids

Did a couple more pea+ F2 bags.. mainly to see if there was the same amount of variation as the tub and appears there is
BF8C2183-7F2B-4593-9EA9-0337B8C2F392.jpeg C4CA3E4D-1490-4203-BE7C-493209F276C2.jpeg

The 2 pea+ f1 clone bags 2nd flush is coming in..
1DCCE49E-7220-4507-80AF-6EAC36C94ACF.jpeg C1A3760B-97F4-4258-8273-AFAB0786CC5A.jpeg

PE/pesa RADS cakes are looking good
809DA1FF-4198-43C7-BA21-219F20F08A25.jpeg

Birthing the PE/apeu RADS cakes tomorrow

Faht
  • ElPirana and YoshiTrainer like this

#330 rockyfungus

rockyfungus

    Mycotopiate

  • Free Member
  • 1,127 posts

Posted 21 April 2021 - 10:43 AM

How often did you need to change that water? Are those darker caps aborting out on you on those f2 pea+?

Just dropped my PEA+ crosses to grain. Changed my grain process a little bit and they are wetter then I like, but I always have backup plates.


Edited by rockyfungus, 21 April 2021 - 02:07 PM.

  • fahtster likes this

#331 DarkNchildlike

DarkNchildlike

    Mycotopiate

  • VIP
  • 1,088 posts

Posted 21 April 2021 - 12:12 PM

I dont think those are aborts they appear to be heavily pigmented caps is all

Even if it ain't all good. It's all good enough.
  • fahtster likes this

#332 fahtster

fahtster

    Oven bag specialist

  • OG VIP
  • 2,254 posts

Awards Bar:

Posted 21 April 2021 - 01:47 PM

Only changed it the once. It’s tap water so it’s just like leaving a glass of water out for a few days.. it wouldn’t get all rank, ya know? The pins on the f1 were just darker.. I’ll snap a pic tonight when I get home.. they looked good this morning; almost done. The F2 bags definitely have aborts happening... that happened with the tub I did too, but I think for F2’s, I’m going to stick to tubs... they seemed to like the space and better FAE than the bags.. I’ll have to test that out with another suspected hybrid F2 other than pea+.. I’ll definitely be using less spores than I did with these.. each qt had 5+ml of heavy SS to each and that’s just WAY to many genetics going on

I prefer my grains on the burst side and load them after only like 20-30 mins in the strainer and don’t have problems, so hopefully yours will be fine. Excited to see what you get!

Faht

Edited by fahtster, 21 April 2021 - 01:54 PM.

  • Sharonlovesu2 likes this

#333 rockyfungus

rockyfungus

    Mycotopiate

  • Free Member
  • 1,127 posts

Posted 21 April 2021 - 02:07 PM

Sorry meant F2, just curious as I tend to get those darker caps and they just never mature. Perfect, yeah they were under-strained and I even towel dry with a beach towel most of the time. Hopefully I get quicker growth. 


Edited by rockyfungus, 21 April 2021 - 02:08 PM.

  • fahtster likes this

#334 fahtster

fahtster

    Oven bag specialist

  • OG VIP
  • 2,254 posts

Awards Bar:

Posted 21 April 2021 - 03:20 PM

Yeah I just didn’t have enough holes in the bags.. the weather got much warmer during these bags life time.. added a bunch more holes and they started turning around but I’ve kinda given up on these f2... I’m going to take the f1 clone that I have to F2 in the fall.. get a few pea+ phenos stabilized and whichever one turns out best, release that one to the masses

Faht

#335 fahtster

fahtster

    Oven bag specialist

  • OG VIP
  • 2,254 posts

Awards Bar:

Posted 21 April 2021 - 07:23 PM

Here’s the end of the PE/pfrs second flush.. they look so much like the pea+ and PE/RW (if they are indeed a hybrid)

5E0F5107-C920-48E3-8323-9E89E66D4F70.jpeg ED9B3442-8626-4751-9201-8EE8FD3BF8B5.jpeg C126877B-04EB-462F-8822-2EBFE02DBFBB.jpeg

^^^ those are a clone that grew 90% sporeless (https://mycotopia.ne...1617851540.jpeg) BUT 3 of them dropped purple spores.. I’m printing them right now. I’m going to share them with 3 ppl here.. but I’ve got some parameters for requesting one of them... you have to:

1. Be confident enough that you can grow them out from a print (if you do agar, that’d be preferable, but I don’t use agar so I that would be a bit hypocritical if I required it)
2. Gotta be in the states.. sorry everyone else.. I just don’t want to send stuff through customs
3. Gotta post results to this thread
4. I haven’t already sent ya something similar.. I think I sent a couple PE/pfrs purple prints out here but I can’t remember.. I’ve sent out too many prints/swabs and I’m getting old lol

If you think you can do all those things, I’ll send ya one.. first come, first serve. This is an f1 clone so I’m hoping you see a red spore fruit in the F2 grow.. if you take the print and then don’t grow it out, we won’t be able to see if it is indeed a red spore hybrid. Wish I had more than 3 but alas I don’t unfortunately... this is all on the probability that all three caps print.. they were all dropping on the stems when I put them to foil an hour ago so it should be all good but just a heads up there’s a chance I won’t have 3.. it’s slim though

Pea+ f1 clone bag #1 second flush
C0C8987E-F798-43BD-B1FC-EE42E43F27CA.jpeg 2DA0DA4D-12FA-424E-A7A4-D4E419AB6301.jpeg

^^^ same culture but in a tester 1/2 pint that I grew in the water tub FC
47A2CBA4-5A4A-4FA2-A685-50E271391E25.jpeg

Here’s the pea+ F2 bags.. should’ve used more holes in the bags and less spores but oh well... still has that crazy variation so, at least I know the tub wasn’t a fluke.
70003CDB-3DB1-48D6-8B5C-2F1214CC525A.jpeg D49F8D62-E33D-4325-B30E-24DDF676C002.jpeg

Faht

Edited by fahtster, 21 April 2021 - 08:12 PM.

  • ElPirana, rockyfungus and Ringo like this

#336 fahtster

fahtster

    Oven bag specialist

  • OG VIP
  • 2,254 posts

Awards Bar:

Posted 22 April 2021 - 08:30 AM

All 3 turned out so we good. :)

571A4308-352E-4922-8794-78A3EF318353.jpeg

Faht
  • ElPirana and UnHeisenbug like this

#337 rockyfungus

rockyfungus

    Mycotopiate

  • Free Member
  • 1,127 posts

Posted 22 April 2021 - 08:53 AM

Did you really gotta show off a curly like that! Do you ever try swabbing gills after you print, that's been my current MO.


  • fahtster likes this

#338 fahtster

fahtster

    Oven bag specialist

  • OG VIP
  • 2,254 posts

Awards Bar:

Posted 22 April 2021 - 09:57 AM

What’s a curly.. you lost me there. I haven’t, but that’s a great idea!

Faht

#339 Ringo

Ringo

    Mycophage

  • Free Member
  • 196 posts

Posted 22 April 2021 - 10:35 AM

Hey gang, sorry for the noob question but is all that fuzz (in the pic with the shrooms growing out of the jar) common? That would freak me out if I saw it growing.

 

Peace



#340 rockyfungus

rockyfungus

    Mycotopiate

  • Free Member
  • 1,127 posts

Posted 22 April 2021 - 10:51 AM

The fuzzy feet at the base of the stem? Some say you need to increase fresh air exchange (fan more often). I wouldn't get too worked up over that, anything colorful or looking like cobweb is another story. 

 

https://mycotopia.ne...615-fuzzy-feet/
 


  • Ringo likes this




Like Mycotopia? Become a member today!