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Crossing strain varieties via swab to spore solution


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#421 fahtster

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Posted 04 October 2021 - 08:30 AM

Yeah.. perfect example of an F2 gen.. that kind of variety is what I’m expecting from the PE/RW F2 cakes.. gonna be birthing those tomorrow and they look primed to start fruiting within the first week (knotting invitro). Stay turned!

Faht
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#422 fahtster

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Posted 06 October 2021 - 04:24 AM

PE/RW F2 cakes birthed.. got a good feeling about these.. they’re CV/brf and also rolled in CV
9319499D-4AE7-44D9-8C44-52BCC15A802C.jpeg

PE/crs F2 cv/brf cakes looking good
714339E8-FEC8-4F8E-851F-B8740C30F665.jpeg

PE/apeu (papu) F2 2nd flush baggie and 1/2 pint cv tester jar
F50820ED-9F90-4DED-B87B-D92F5BFEE9BD.jpeg AADCF44C-F780-4C44-8894-4461E4B830CB.jpeg

Faht

Edited by fahtster, 06 October 2021 - 04:27 AM.

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#423 rockyfungus

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Posted 06 October 2021 - 05:13 PM

it appears some rice got into your papu


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#424 fahtster

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Posted 09 October 2021 - 05:57 AM

Pea+ f5
2D8A5117-1C39-4F90-9E38-4EA7340781A0.jpeg 8951EE1A-27A1-4541-8F8B-E3F00148D350.jpeg

Papu tester jar 2nd flush
A3C8E74C-416C-4046-B364-A4819CD2B23C.jpeg

Papu 16qt 2nd flush is coming in soon

Faht
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#425 fahtster

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Posted 10 October 2021 - 03:34 AM

Pea+ f5 tub looking good
C86AE800-30EE-4317-822C-A67BCC25AF1D.jpeg 6F558703-CB0B-4BE0-941A-421F46FF304D.jpeg

Cakes looking good as well
AD9F5542-70F8-40F5-B990-C39007057974.jpeg

I’ve made a cross back to PE swab but it’s looking pretty stable.. couple more gens and I think it’ll be be ready for a giveaway :)

Cool PE/apeu (papu) f2 2nd flush
AC5A5579-15D0-4C15-BE2B-05A5CEE01D11.jpeg 321F19BE-F516-4D59-8BD9-F30E949CDFB4.jpeg

16qt papu 2nd flush coming in
D5A4CA3F-7D57-4093-B78F-19A376919B49.jpeg

Faht

Edited by fahtster, 10 October 2021 - 06:11 AM.

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#426 rockyfungus

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Posted 10 October 2021 - 08:29 AM

"Cool PE/apeu (papu) f2 2nd flush"

 

Dude those are wild. Those stems, the veil, can you do a shot from top down? My brain doesn't see a cube.
Maybe an artifact from the background just confusing the shit out of me, first glance I thought they were Mex or exotics with huge caps...


Edited by rockyfungus, 10 October 2021 - 08:31 AM.

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#427 fahtster

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Posted 10 October 2021 - 11:13 AM

Best I got.. flash just washed it out
33673046-1933-46AE-8029-822A379847AC.jpeg
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#428 rockyfungus

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Posted 10 October 2021 - 11:50 AM

Those are gorgeous, digging the stem. Thanks!
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#429 fahtster

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Posted 10 October 2021 - 04:02 PM

Last money shot before the dehydrator of the pea+ f5
623F6E3D-F7AC-4B7F-993B-D9E84389E67D.jpeg

Faht
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#430 fahtster

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Posted 11 October 2021 - 09:54 PM

PE/apeu and PE/RW F2 2nd flush coming in
E52612D7-690F-44B5-B269-4FAA6657CAC7.jpeg 698032BB-5EAB-4470-9049-74AE323569F5.jpeg

Pea+ f5 baggies 2nd flush
D94EA121-5876-44F3-8CE7-08FF6566BDB4.jpeg

PE/pfrs f1 clone lrg ziplock coming in.. had to expand the bag due to side pins
020C8691-11A6-456C-85C9-2C53A079FDE4.jpeg 897E15AB-1F91-4134-9C1C-61E4ABFAA1B2.jpeg

Faht
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#431 fahtster

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Posted 12 October 2021 - 05:44 PM

PE/apeu (papu) f2 second flush.. man, they make a beautiful fruit… I totally take back on not doing work on them :lol: I’m convinced there’s pigmented fruits putting out clear spores.. I think I can tell spored gills from sporeless gills.. there’s a film with a softness to the gills that others don’t have. These puppies were strong and had a great pin set so on to f3!
3807F930-669D-462A-8215-2531000B5381.jpeg C518842A-F957-4BC4-84B9-175B1A9924CD.jpeg B8D157B2-C045-43F7-B65A-E8141098587B.jpeg D1C3CA33-D302-470C-99FE-3A9F4FD0E438.jpeg

Looks like the chimera clone I grabbed, took, so there should be a tub of those in the near future :grin:

I’m 100% on these being a hybrid along with the pea+. I’m 99% on the PE/RW but I want to see the cakes that are coming up and possibly the f3 I’m making GLC grains for rn.. the 2nd flush of the tub is about done and I think that’ll bump the 99 to a 99.99% but I want to be totally sure before I claim that
Comparison pics for ya:

Apeu MS……….PE ms………Papu ms
D9F2821E-B0A9-457B-A798-CE66AC78AABA.jpeg 00A719BD-BFD2-4B86-9CB0-D67450FA4176.jpeg 003868FB-9012-4AE6-BE7C-C1B707C8B5DE.jpeg

Faht

Edited by fahtster, 12 October 2021 - 05:54 PM.

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#432 fahtster

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Posted 13 October 2021 - 01:32 AM

PE/RW F2 second flush… more diversity coming out in this flush. Most of the white ones are dropping purple and most of the pigmented are dropping rusty.. I’m jar printing the bigger pigmented (rusty) cap @ 7 o’clock
9D410ED6-C045-42C6-8CBA-FC83D956AD2A.jpeg

Like I thought, these guys pretty much got me @ 99.99% sure.. I believe most if not all RW “reverts” I’ve seen ppl post are pigmented purple spored fruits.. most pigmented of these are dropping rusty.. and there’s definitely PE stem bumpage
ADAFEB35-0BC1-4033-8362-0896D9D56185.jpeg 6ED7CBA1-7A17-47EC-AA6E-EBF4BD732998.jpeg

The cakes are starting to pins so that should help

Faht

Edited by fahtster, 13 October 2021 - 01:33 AM.

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#433 MushLuvR

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Posted 13 October 2021 - 10:18 AM

My Homestead PE has those same crinkled stems(your stem looks dense to), definitely PE influenced, and that BIG cap, like a flying Saucer(RW it looks like) or something, wonderful looking and awesome work... AS usual.   :thumbs_up:

 

IMG_2193.jpg

IMG_2194.jpg

So many different kinds of Mutations...

I enjoy the variation personally.  


Edited by MushLuvR, 13 October 2021 - 10:25 AM.

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#434 fahtster

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Posted 13 October 2021 - 11:59 PM

PE/pfrs bag gettin there.. these guys are dense af too :)
7ACD8DB5-096E-4E03-9874-EA943C61E053.jpeg 02344FC4-3EA8-4979-B953-1ADA01667161.jpeg ECBD41EA-CFF4-4D7E-9109-4538ED2EFD87.jpeg

PE/RW F2 cakes starting to pin
DC76E508-A866-42DC-B041-577ECAAB9AF2.jpeg

Inoculated a PE/RW f3 glc jar tonight

Faht
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#435 fahtster

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Posted 14 October 2021 - 07:32 PM

PE/pfrs f1 Flush 1 done
4444270B-030A-4962-A855-23DCFDC151FC.jpeg 38D9E822-13E3-40C2-A896-DBF808A0166C.jpeg

Faht
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#436 fahtster

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Posted 19 October 2021 - 03:00 AM

PE/RW F2 cakes today
3E1F5E8F-3212-4E15-B4B6-94AA54A46DED.jpeg

Faht
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#437 fahtster

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Posted 20 October 2021 - 08:45 PM

PE/RW cakes.. last shot.
86DEED04-406D-4DDD-99FA-C934FD15D106.jpeg

Lots of variation.. I was convinced of the hybrid before these but they help.. this was actually from a second f1 print than the tub fruits so the odds of getting all this variance with two separate prints is low I’d say

I’m about to shake the f3 glc jar.. excited to see what comes out of those :)

Faht
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#438 fahtster

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Posted 21 October 2021 - 11:01 PM

Pea+ f5 second flush
A0D7A7EA-02AE-4592-AE69-B11EEB2D3BAA.jpeg

Pe/crs baggie.. got 4 cakes almost done colonizing
B0150E95-DCFA-4490-A8CA-6CAF2605DA95.jpeg

Faht
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#439 fahtster

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Posted 26 October 2021 - 02:09 AM

PE/RW 3rd flush chonker and a weird lil guy
801C76B1-DC6A-41FE-8BE1-756767A4E9E0.jpeg 711255D2-06FD-46A4-9680-B0AE3D026185.jpeg

PE/crs baggie.. no spore drop.
F55BD9B4-4EF9-4F80-9A44-1069B87520E9.jpeg
4BF660E5-469E-4B9A-AC7B-15B5B840ADCC.jpeg

PE/apeu 3rd flush
67F6BDEC-1AD9-4EB3-9AFE-CD2D0BBDBD92.jpeg

Faht

Edited by fahtster, 26 October 2021 - 02:11 AM.

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#440 YoshiTrainer

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Posted 26 October 2021 - 04:00 PM

Wow Faht your grows are looking amazing....as usual! Really some interesting fruits you are finding too.

No where near as photogenic but thanks to a very kind individual I've had luck fruiting a few crosses too. I started by putting PE/APEU & PE/RW to agar and quickly moved them to grain then to bulk. Unfortunately my fan hadn't been cleaned in awhile and the bulk bags seem like they picked up bacteria. I left them sitting and they surprised me with fruits, still in the bag. With my fan now clean, I opened the bags and dropped them in shoeboxes with no misting or fanning.

Here are the F-2 PE/APEU tubs

Freshly cut bags

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Shoe boxes of PE/APEU

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35.9g cracker dry

20211025_131744-1.jpg

I did take a few prints but no clones. I might run the F-2 again or maybe just move to F-3?

The PE/RW is looking more bacterial but still popped a few fruits

20211021_223714.jpg

20211026_135539-1.jpg

I have 6 clean bags of PE/PFRS F-2, hopefully getting ready to pin in the near future. I will have more time to play with those (I hope!).
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