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Crossing strain varieties via swab to spore solution


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#541 fahtster

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Posted 04 January 2022 - 03:47 AM

PE/F+ cakes chugging along.. making a couple qts for the glc jar tomorrow.. the glc jar is looking good.. it’s been shaken a couple times now. If there was mold, it’d have shown up by now.
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I’m excited for these.. it’s been almost a year since my last hybrid attempt.

Starting to see some germ in the pea+ f7 cakes and glc jars :)

Faht
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#542 fahtster

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Posted 09 January 2022 - 01:49 AM

3rd flush of the pea+ f6 16qt tub
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The f7 glc jars are almost 100% and the cakes look good with good germ.. here they are a couple days ago
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PE/f+ cakes are @ about 70% and I knocked up 2 qts of wheat with the glc jar
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PE/RW f4 qts are looking good a day after a shake
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Started PE/pesa f3 and PE/apeu f4 (2 cakes and a glc jar of each)

Faht :)

Edited by fahtster, 09 January 2022 - 03:07 AM.

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#543 fahtster

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Posted 11 January 2022 - 02:51 AM

GLC’ing one of these pea+ f7 guys to 2 wheat qts tomorrow
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After I use it, I’ll let it recover and then spawn it to a sandwich baggie to get f8 spores and/or clone material.. the second one will follow once it’s 100% as a back up… the baggies in general are a back up in case the qts and/or cakes don’t work out. Gotta cover the bases when going spores to grain

Faht

Edited by fahtster, 11 January 2022 - 02:52 AM.

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#544 fahtster

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Posted 15 January 2022 - 03:48 PM

Gonna be focusing on cross work for the rest of the season..

PE/F+ cakes @ 100%.. waiting on knots. The glc grains baggie is also waiting on knots. Shook the qts yesterday
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Pea+ f7 cakes starting to take off.. made a sandwich baggie yesterday
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PE/pesa f3 and PE/apeu f4 cakes (2 of each) showing germination.. shook each of their glc jars yesterday
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Made the PE/RW f4 tub yesterday and the sandwich baggie is awaiting knots
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Side note.. make sure you check the box when you buy baggies to make sure they aren’t colored lol

Faht
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#545 fahtster

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Posted 25 January 2022 - 10:47 AM

Got a pea+ f5 clone tub pinning.. this is from a thread I did on another site where I’m showing that you can dry out bacterial cultures to clean them up (as long as they’re not endospore producing bacteria.. there’s surprisingly few families of bacteria that produce endospores). Once the thread is finished, I’ll most likely post it here.. it’s tough keeping up multiple threads on various topics on various sites lol

Here’s a sneak peak though..

Bacterial f5 clone culture
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Dried it out (using my specialized lids that allow for a lot of GE) for 3 weeks
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GLC’d that to new qts
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New tub is now pinning
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Faht
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#546 fahtster

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Posted 01 February 2022 - 10:35 AM

The pea+ f5 clone tub did pretty well.. they were nice dense fruits, got 71g off it
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PE/RW f4 baggie putting out lots of blobs.. got a cased 16qt that’s putting out some regular pins thankfully
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PE/F+ f1 attempt baggie pinning… the cakes are also starting to pin and I cased the 16qt a couple days ago.. these early pins are probably f+ or a cross so I’ll have to see what they look like mature
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Faht
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#547 fahtster

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Posted 04 February 2022 - 05:05 AM

PE/F+ baggie has some candidates that I’d like to see the F2’s of… 16qt should be fun
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Cakes are definitely gonna have some fruits to take to the F2.. gonna be some chunkers too
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Faht

Edited by fahtster, 04 February 2022 - 05:07 AM.

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#548 fahtster

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Posted 06 February 2022 - 12:14 PM

PE/RW f4 ms coming in.. these will all be rusty (f3 was with mixed Lucy and pigmented fruits but chose pigmented to stabilize because I want to keep it on the PE end of the spectrum for potency and I think going pigmented gives me the best chance for that)
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PE/F+ f1 off a cake.. jar printing a section of the cap (took a swab too).. really like the looks of this guy.. nice and bumpy
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Faht
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#549 fahtster

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Posted 08 February 2022 - 10:01 PM

PE/RW f4 tub about done.. waiting on the pheno I want to drop spores
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The bumpy cap and stipe pheno is the one I’m after for f5
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They’re all producing rusty spores at this point
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Faht

Edited by fahtster, 08 February 2022 - 10:02 PM.

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#550 fahtster

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Posted 09 February 2022 - 05:55 PM

The pea+ f5 clone tub I revived from dried out bacterial spawn 2nd flush
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Faht
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#551 fahtster

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Posted 09 February 2022 - 10:05 PM

The PE/RW f4 tub was chopped down.. it’s gonna give some of my PE tubs a run for their money BE wise and it’s heavy ms. Excited to get a later gen clone and see what it can do. Wish I would’ve known they weren’t gonna drop spores, I woulda harvested sooner. “If ya didn’t know, now ya know… Fahtster”
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#552 fahtster

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Posted 11 February 2022 - 03:43 AM

That tub dried down to 99g on the dot (used 2qts of wheat). that’s better than any swpe tub I’ve done so far and the PE/RW was heavy MS.. stoked to see this one through
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#553 fahtster

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Posted 12 February 2022 - 08:56 PM

PE/F+ f1 16qt is looking noice.
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Faht
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#554 fahtster

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Posted 14 February 2022 - 08:13 AM

PE/pesa f3 baggie looking intense
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PE/apeu f4 still has a ton of diversity (or isn’t as high ms as I thought.. I’ve been swabbing pigmented fruits)
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Doing a qt of each of those in a single 16qt tub.. here it is precasing.. should be a neat tub if they fruit at the same time
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PE/F+ tub today
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Fun stuff :)

Faht

Edited by fahtster, 14 February 2022 - 08:16 AM.

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#555 fahtster

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Posted 14 February 2022 - 03:53 PM

Update on pea+ f7

The cased 16qt is looking ready to start knotting. One thing about pea+ is that the spores seem to be more “fertile”. Spores germ extremely readily even from the f1… this is good of course but it also makes SS very tomentose looking.. there’s so many sub strains because a ton of spores germ in a small area that myc grows super thick looking. This is also what’s giving this tub almost a pan myc look
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F7 cakes coming in.. I already grabbed a clone of a nice fat fruit off the 3rd cake just out of the pic
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I’ve also started incorporating josex’s brf puck method.. I put a piece of clone tissue all the way to one side of the jar and I’m going to let that run as far as it can and then I’m going to grab some leading edge myc and transfer that to a 4oz grain jar.. hopefully this will further make sure the clone culture is free of bacteria.
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#556 fahtster

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Posted 15 February 2022 - 09:54 PM

PE/F+ f1 cross attempt 16qt.. some neat phenos.. I’ve seen it a lot in the PE crosses (still don’t know that it’s a cross but it’s the one I’m going to take for an F2)
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PE/apeu f4 baggie a bit nicer on the pin production than the f3 but still lots of variation (been swabbing pigmented fruits).. hoping in the next couple gens they fatten up a bit but we’ll have to see what happens.. might end up going back to the F2 and picking a fatter candidate
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Pea+ f7 cakes.. cloned one of the fat guys on the bottom.. it’s got everything I’m looking for.. late veil drop, producing spores, solid and gnarly stem and two toned cap.. cloning went super smoothly.. really stoked about it
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Pretty happy with the 2 clones I’ve taken so far.. enough that I can relax and not worry about how the 16qt is going to turn out so much… I am excited to see how ms does with this gen tho.

I’m actually more excited about the 2nd flush of the tub for the larger fruits and ease of cloning them

Faht
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#557 fahtster

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Posted 16 February 2022 - 10:10 PM

PE/F+ f1 tub done.. jar printed the largest gnarly capped one.. gonna swab a few of the other weird PEsque ones.. between these and the cakes, I’ll have plenty to work with to see what a F2 shows
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Faht
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#558 fahtster

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Posted 21 February 2022 - 01:44 AM

Pea+ f7 ms tub coming in.. pinning like crazy. Hope ppl can dig that.. let see how big they get
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I’ve already got clones colonizing glc jars of the f7 off cakes.. those tubs will be the ones I swab off of for the giveaway. Unless something better shows up in the tub (not expecting much different than what I’ve already cloned). Getting real close now :)

Faht
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#559 Arathu

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Posted 21 February 2022 - 07:00 PM

I'm just going to pseudo run out of likes in this thread and make a BIG one here as a post (see what I did there?)......hahahaha Look-at-em! Geezus....

 

Wonder if that exerts an upward force....I bet it does....

 

Well done man!

 

A


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#560 fahtster

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Posted 22 February 2022 - 01:55 AM

Thanks pal! Yeah hopefully they get to a decent size.. fingers crossed
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Dual variety 16qt tub starting to kick off.. PE/pesa f3 on the left, PE/apeu f4 on the right
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Faht
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