Crossing strain varieties via swab to spore solution
Posted 06 January 2021 - 11:41 AM
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Posted 09 January 2021 - 04:11 AM
Last f1 ms tub
Tester 1/2 pint of a 4oz rye spawned to CV looking good for a pin set..
^^^that has me excited for the two tubs of that culture I have about to flush.. I did a late casing on one half and a 1/2” CV top layer on the other.. trying to figure out what this culture likes best. I’ve got two more tubs of the clone in the works that I’m going to case with 50/50+ for one and a really thin top layer on the other
The bin of PE/pfrs RADS is almost fully colonized.. put its cakes in a FC on wed too. That’s got me really excited.. finally (hopefully) get to see if I can replicate getting another cross like I did with pea+... mere days away. Won’t be as easy to tell the difference between pfrs and a PE/pfrs cross as it was with PE and AA+ though.. hoping it’s obvious
Edited by fahtster, 09 January 2021 - 04:16 AM.
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Posted 10 January 2021 - 06:42 PM
So I’ve got the PE/pfrs RADS (rub and double swab) in fruiting chambers. 16qt tub, 2 qts of rye spawn to CV.. The qts were inoculated with GLC made with the RADS SS. It’s been 4 days
The last time I did this^^^ with pea+, I got an entire bin of cross fruits, so here’s to hoping!
Here’s the coir cakes I made with the RADS SS.. it was the first time attempting coir cakes and I made them too wet.. I ended up having to cut off some uncolonized areas at the bottoms of the cakes so hopefully I went far enough and they don’t tam out.. I’m hopeful though. They’ve also been in the FC for 4 days
So hopefully in the next couple weeks I’ll know if I got my coveted red spore PE variety. :)
Today I also put my pea+ f1/pfrs RADS brf cakes to a FC. I figured the best possible thing that could happen trying to cross the f1 pea+ with pfrs is, I could end up with a red spore pea+, a red spore AA+, and a red spore PE and the worst thing that could happen is I end up with an F2 pea+.. seems like a win-win to me, so I said fack it, let’s try lol
Those cakes colonized great and look awesome.. they reminded me of the first pea+ RADS SS cakes I made and got crosses at almost every inoc point, so I’m pretty excited about this grow. The qts I made with the glc of that RADS SS we’re shook yesterday and they looked great pre-shake
When I made the glc grain jars for those^^^ qts, I made two 4oz rye jars... one I used to inoculate those qts and the other I spawned to CV in a little glad container and it’s probably a fewish days away from knots atm..
That’s why I like those little testers.. they give me a glimpse of what’s to be in a couple weeks
Lots of fun and exciting possibilities coming up in the next couple weeks! Really need the mush gods to shine on me lol
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Posted 11 January 2021 - 10:14 PM
This cloned fruit
Got a couple tubs fruiting now.. they don’t look as good as the tester but I’m thinking it’s because the top layer is too thick or just needs a real thin one
Here’s the 16qt clone tub
The whole tub has a 1/2” top layer and the right side has a late casing at knots.. I think a 1/4” peat casing will do well on one of the next tubs.. I’m going to try no top layer or casing in at least 1/2 of another tub
Edited by fahtster, 12 January 2021 - 01:43 AM.
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Posted 11 January 2021 - 11:54 PM
No one is using single spore monokaryotic cultures to create a fruiting substrain. How is it even possible to "cross" two p.cubensis without knowing what fruiting substrain you're dealing with?
I get the theory that its during the natural selection process of fruiting substrains is where some mutations or anomalies might manifest themselves and make some of the mushrooms look new but guaranteed it can happen even if you're not crossing strains.
Printing from those caps will not guarantee that the same anomalies will carry over in a new monokaryotic culture even if you start one from a single spore.
It would if it was a tissue clone.
Now, can someone tell me what p.cubensis were mixed together to create "jack frost" cause I want some p.cubensis that produce blue spores and I'm willing to try the experiment without high expectations but I need to know exactly which spores were mixed and matched from what original p.cubensis novelties?
Posted 12 January 2021 - 12:13 AM
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Posted 14 January 2021 - 12:17 AM
Some clone pics..
The late cased/top layered side is filling in nicely
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Posted 15 January 2021 - 12:52 AM
Ms pea+ 2nd flush
I’ve got pins in my PE/pfrs cross attempt coir cakes and the 16qt is look like it’s about to knot. Also have pins on the brf pea+ f1/pfrs cross attempt cakes and knots on the little glad container. Gonna be a fun week
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Posted 16 January 2021 - 04:44 AM
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Posted 16 January 2021 - 06:57 AM
Damn tbh thats what I hoped to see when I said "I'm more exited to see the f1".
Hope you stabilize those nipple heads rather than the other tubs you've shown.
I personally would love to see a pheno with thick PE stem and those caps
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Posted 16 January 2021 - 12:38 PM
Edited by fahtster, 16 January 2021 - 12:39 PM.
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Posted 16 January 2021 - 02:05 PM
Favorite sorta out of context Quote of day ................
" I Expect To See Nipple Heads ! "
Ha Ha .... just made me chuckle ...
Edited by bezevo, 16 January 2021 - 02:06 PM.
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Posted 16 January 2021 - 02:50 PM
That clone tub dried down to 66.4gms.. right on par with my PE tubs and only half the tub fruited (even though I’m sure the unfruited side probably supplied a lot of water and support for the fruiting side, but I’m confident I can get that around 80gms if I have full pin coverage.
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Posted 16 January 2021 - 08:00 PM
I will be stabilizing this clone for sure but I expect to see nipple heads in the f2 tub that’s coming up as well.
Whats your process for stabilizing clones, if you dont mind me asking? You want those nice nipples and thick stems as the desired phenotype?
Posted 17 January 2021 - 01:16 PM
So.. I had a pea+ 16qt sub that fruited the first flush and I needed the tub it was in to start a new sub but wanted to take the first sub to a second flush, so I put the sub into a 2.5 gallon ziplock and poked needle holes every 2-3” on top and sealed it up.. put it on an old tub lid and put it on a shelf and forgot about it
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Posted 19 January 2021 - 11:33 PM
Just in case there’s still doubt that pea+ is a PE/AA+ cross. I’ll definitely post more pics as they progress
Here’s the straight F2 pea+ tub.. it’s knotting up.. should see pins in the next couple days
The pe/pfrs cross attempt coir cakes are pinning.. really liking how the coir cakes are pinning hard.. definitely going to attempt coir cakes again
The 16qt of the PE/pfrs is also pinning
Fun stuff! :)
Edited by fahtster, 20 January 2021 - 12:09 AM.
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