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Crossing strain varieties via swab to spore solution


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#121 sandman

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Posted 03 January 2021 - 06:20 PM

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#122 fahtster

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Posted 06 January 2021 - 11:41 AM

F2 pea+ spores to qts going well

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Faht
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#123 fahtster

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Posted 09 January 2021 - 04:11 AM

Pea+ F2 qts are just about done.. might give it til Sunday before I spawn them
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Last f1 ms tub
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Tester 1/2 pint of a 4oz rye spawned to CV looking good for a pin set..
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^^^that has me excited for the two tubs of that culture I have about to flush.. I did a late casing on one half and a 1/2” CV top layer on the other.. trying to figure out what this culture likes best. I’ve got two more tubs of the clone in the works that I’m going to case with 50/50+ for one and a really thin top layer on the other
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The bin of PE/pfrs RADS is almost fully colonized.. put its cakes in a FC on wed too. That’s got me really excited.. finally (hopefully) get to see if I can replicate getting another cross like I did with pea+... mere days away. Won’t be as easy to tell the difference between pfrs and a PE/pfrs cross as it was with PE and AA+ though.. hoping it’s obvious

Faht

Edited by fahtster, 09 January 2021 - 04:16 AM.

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#124 fahtster

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Posted 10 January 2021 - 06:42 PM

I spawned the F2 qts today to a 16qt bin.

So I’ve got the PE/pfrs RADS (rub and double swab) in fruiting chambers. 16qt tub, 2 qts of rye spawn to CV.. The qts were inoculated with GLC made with the RADS SS. It’s been 4 days
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The last time I did this^^^ with pea+, I got an entire bin of cross fruits, so here’s to hoping!
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Here’s the coir cakes I made with the RADS SS.. it was the first time attempting coir cakes and I made them too wet.. I ended up having to cut off some uncolonized areas at the bottoms of the cakes so hopefully I went far enough and they don’t tam out.. I’m hopeful though. They’ve also been in the FC for 4 days
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So hopefully in the next couple weeks I’ll know if I got my coveted red spore PE variety. :)

Today I also put my pea+ f1/pfrs RADS brf cakes to a FC. I figured the best possible thing that could happen trying to cross the f1 pea+ with pfrs is, I could end up with a red spore pea+, a red spore AA+, and a red spore PE and the worst thing that could happen is I end up with an F2 pea+.. seems like a win-win to me, so I said fack it, let’s try lol
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Those cakes colonized great and look awesome.. they reminded me of the first pea+ RADS SS cakes I made and got crosses at almost every inoc point, so I’m pretty excited about this grow. The qts I made with the glc of that RADS SS we’re shook yesterday and they looked great pre-shake
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When I made the glc grain jars for those^^^ qts, I made two 4oz rye jars... one I used to inoculate those qts and the other I spawned to CV in a little glad container and it’s probably a fewish days away from knots atm..
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That’s why I like those little testers.. they give me a glimpse of what’s to be in a couple weeks

Lots of fun and exciting possibilities coming up in the next couple weeks! Really need the mush gods to shine on me lol

Faht
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#125 fahtster

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Posted 11 January 2021 - 10:14 PM

lil tester clone 1/2 pint of rye to CV..
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This cloned fruit
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Got a couple tubs fruiting now.. they don’t look as good as the tester but I’m thinking it’s because the top layer is too thick or just needs a real thin one

Here’s the 16qt clone tub
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The whole tub has a 1/2” top layer and the right side has a late casing at knots.. I think a 1/4” peat casing will do well on one of the next tubs.. I’m going to try no top layer or casing in at least 1/2 of another tub

Faht

Edited by fahtster, 12 January 2021 - 01:43 AM.

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#126 FunG

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Posted 11 January 2021 - 11:54 PM

I gave my opinion on "crossing" p.cubensis in another thread but I have to echo thefunkyones opinion is the same as mine.

No one is using single spore monokaryotic cultures to create a fruiting substrain. How is it even possible to "cross" two p.cubensis without knowing what fruiting substrain you're dealing with?

I get the theory that its during the natural selection process of fruiting substrains is where some mutations or anomalies might manifest themselves and make some of the mushrooms look new but guaranteed it can happen even if you're not crossing strains.

Printing from those caps will not guarantee that the same anomalies will carry over in a new monokaryotic culture even if you start one from a single spore.

It would if it was a tissue clone.

Now, can someone tell me what p.cubensis were mixed together to create "jack frost" cause I want some p.cubensis that produce blue spores and I'm willing to try the experiment without high expectations but I need to know exactly which spores were mixed and matched from what original p.cubensis novelties?

#127 fahtster

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Posted 12 January 2021 - 12:13 AM

Well.. I’ll hopefully be able to show you with the F2 tub I have coming up and I can see some variation.. it’s not from this clone.. this is just a clone I’m playing with. I used PE and AA+ specifically so I’d be able to tell what’s up. Did you read the thread? Spores from each were physically mashed together and when spores germinate, they are mono, no? So if two spores germinate next to each other, can they not combine? That’s idea here
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#128 fahtster

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Posted 14 January 2021 - 12:17 AM

F2 tub is coming along nicely
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Some clone pics..
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The late cased/top layered side is filling in nicely
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Faht
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#129 fahtster

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Posted 15 January 2021 - 12:52 AM

Other clone tub
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Ms pea+ 2nd flush
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I’ve got pins in my PE/pfrs cross attempt coir cakes and the 16qt is look like it’s about to knot. Also have pins on the brf pea+ f1/pfrs cross attempt cakes and knots on the little glad container. Gonna be a fun week

Faht
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#130 fahtster

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Posted 15 January 2021 - 12:31 PM

Who doesn’t like pics? Lol clone..

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2nd flush bigguns
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Faht
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#131 fahtster

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Posted 16 January 2021 - 02:52 AM

I’m in love with this clone and it’s only the first one of many to come lol.. final shots of this tub. Easily gonna clear 2oz

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Faht
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#132 DarkNchildlike

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Posted 16 January 2021 - 04:44 AM

Thats so funny I just messaged you about something related to breeding with PE. Depending on what characteristics you develop will you back cross again with original PE to gain some more of its traits? I suppose there is no need to if you like what you are getting tho :) this is super interesting I love reading about breeding projects!
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#133 Moby

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Posted 16 January 2021 - 06:57 AM

Damn tbh thats what I hoped to see when I said "I'm more exited to see the f1".

Hope you stabilize those nipple heads rather than the other tubs you've shown.

 

I personally would love to see a pheno with thick PE stem and those caps


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#134 fahtster

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Posted 16 January 2021 - 12:38 PM

I will be stabilizing this clone for sure but I expect to see nipple heads in the f2 tub that’s coming up as well.

Faht

Edited by fahtster, 16 January 2021 - 12:39 PM.

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#135 bezevo

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Posted 16 January 2021 - 02:05 PM

Favorite sorta out of  context Quote of day ................

 

"  I Expect To See Nipple Heads  !   "

 

Ha Ha .... just made me chuckle ...

BEZ


Edited by bezevo, 16 January 2021 - 02:06 PM.

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#136 fahtster

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Posted 16 January 2021 - 02:50 PM

Haha.. there’s so many things to do with this hobby that make me say things I wouldn’t have imagined saying before I got into it.. especially when working with penis envy <—- hell, that’s one right there.. I.e. “I have penis envy” etc. lol

That clone tub dried down to 66.4gms.. right on par with my PE tubs and only half the tub fruited (even though I’m sure the unfruited side probably supplied a lot of water and support for the fruiting side, but I’m confident I can get that around 80gms if I have full pin coverage.

Faht
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#137 cujoloki

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Posted 16 January 2021 - 08:00 PM

I will be stabilizing this clone for sure but I expect to see nipple heads in the f2 tub that’s coming up as well.

Faht

 
Whats your process for stabilizing clones, if you dont mind me asking? You want those nice nipples and thick stems as the desired phenotype?



#138 fahtster

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Posted 17 January 2021 - 01:16 PM

Take the spores from the clone and grow them out and pick a fruit with the same pheno type.. grow those spores out and pick the same pheno type and repeat until only that pheno type presents itself from a multi spore grow.. that happens probably around The 8th or 9th generation (so I’m told)

So.. I had a pea+ 16qt sub that fruited the first flush and I needed the tub it was in to start a new sub but wanted to take the first sub to a second flush, so I put the sub into a 2.5 gallon ziplock and poked needle holes every 2-3” on top and sealed it up.. put it on an old tub lid and put it on a shelf and forgot about it

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Faht
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#139 fahtster

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Posted 18 January 2021 - 01:36 AM

Here’s the end of that subs 2nd flush.. they’re chunky!

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Faht
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#140 fahtster

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Posted 19 January 2021 - 11:33 PM

So on my pea+ f1/ pfrs cross attempt (any pea+ fruits would be F2 and I’d expect parent variants in non-pea+/pfrs cross fruits) brf cakes I have an AA+ pin.

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Just in case there’s still doubt that pea+ is a PE/AA+ cross. I’ll definitely post more pics as they progress

Here’s the straight F2 pea+ tub.. it’s knotting up.. should see pins in the next couple days

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The pe/pfrs cross attempt coir cakes are pinning.. really liking how the coir cakes are pinning hard.. definitely going to attempt coir cakes again

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The 16qt of the PE/pfrs is also pinning

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Fun stuff! :)

Faht

Edited by fahtster, 20 January 2021 - 12:09 AM.

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