114 replies to this topic

[fahtster]

No.. well.. kinda lol. the drying happens after the jar is colonized.. and that’s only for storage.

I pc a 4oz jar with hydrated grain like any other grain jar and then add inner stem tissue.. once that’s colonized, I either inject it with sterile water and shake it about to knock the myc off into the water and retrieve to inoculate qts and/or new 4oz jars (to continue the culture further) or I dry it out using one of my lids from here https://mycotopia.ne...he-test-of-time and then retrieve the dry myc using sterile water and shaking with syringe extraction etc.

Faht

Edited by fahtster, 09 December 2020 - 02:48 PM.

[fahtster]

Next round of rye pec2 jars, 3 days after shake



The three clones I took from the first MS PE tub from earlier in the thread spreading their wings



Two clones 1 day after shake

those came from this guy


^^^^ that’s what I’m most excited about rn.. can’t WAIT to get that culture rocking.. I want to get away from straight PE (the long wait times and having to case with jiffy) but still have a potent fruit. Excited to see how that holds up. The only downfall would be that if they grow too big, I’m going to have to switch back to the taller mini monos.. I thinking they won’t get as big as that cake fruit though. Pea+ has about a 17 day spawn to harvest and you don’t need to case; only a CV top layer.

Pea+ f1 laymixed is knotting hard.. probably see primordia in the next day or two



Final rye clone tub dried down to 63.8gm, so:

Tub 1: 65.8
Tub 2: 59
Tub 3 63.8

Three tub total: 188.6gm or 6.7oz

Faht

Edited by fahtster, 09 December 2020 - 11:01 PM.

[Stencill86]

Very nice results! Do you keep them around for more flushes?

[fahtster]

Thanks! Depends.. if there wasn’t full coverage on the surface the first flush.. that first ms tub where there was a decent amount of surface that didn’t fruit, I’m taking it to a second flush and the pea+ that are coming up I’ll probably take further than the first flush, but for the PE, they’re usually one and done.. they just take too long. Like I say in the op, sometimes I get lucky and there’s a nice second flush but for the most part, it’s usually like 1-3 mushies and I’d rather use that time to get another first flush from a new sub.

Faht

[Stencill86]

Nice, I hear that. I’ve never done PE but I always like to chuck em after 2, just in case the first was finicky. Space is pretty valuable in this hobby lol.

[fahtster]

Early bird on the first laymixed 16qt pea+ f1 tub.. I have a feeling friends are in the way... I have 3 of these with 2 being laymixed but this guy was made a day before the other two.. this one is 13 days old



Putting the pea+ cake fruit clone glc to qts tomorrow.. probably the one on the right (making new jelly jars of glc grains I’ll inoculate along with the qts to continue the culture for later tubs) and the left one is going to be dried out in case I ever want to return to square one (in case the first expansion gets tired sometime down the road



Also spawning those next 6 rye Pec2 qts tomorrow as they’re @100%. Also thinking about making a PE/crs double swab into SS and putting it to cakes. I’ve also got germ on a PE/pfrs double swabbed jelly jar I did a few days ago. Exciting stuff!

Faht

Edited by fahtster, 11 December 2020 - 10:38 PM.

[Freaky]

Loving the progress on this Faht!  And I can't say enough on how much I love the small Faht jars and lids for saving masters and for expanding through glc's to grain.  They've really given me something I was missing before when it came to storing cultures easily.  

 

I can't wait to see that pea+ laymix in a few days!

[fahtster]

Thanks freaky.. I’m glad you like my little jars! They really did change everything for me.. I hope they serve you well!

New set of pec2 rye bins.. gonna be the last ones of this culture for a bit.. gonna focus on the pea+ and I have more PE fruits than I know what to do with.. thanks god for vacuum sealers. Lol. Any new PE I do will be the clones I took from that ms tub.



The second set of spelt pec2 are almost ready for their second 1/2” casing layer.. at which time I’ll remove the MPT



The laymixed pea+ is looking gooood for pins.. not much difference but I can feel em coming lol.. it’s knot city

Also turned a pea+ f1/pfrs double swab into SS and inoc’d some cakes and a faht jar.. was gonna do a PE/crs swab I had but then got to thinking, what if all the variations of pea+ F2 mated with pfrs and I got a AA+, PE, and a pea+ fruit that all had red spores from one grow? That’d be amazing, so I’m gonna try and see what happens :)

Speaking of which.. look what I just got



Awww yeah! Gonna be busy making lids tonight.. gf doesn’t look pleased lol. She hates the smell of silicone.. smells like success to me

Faht

Edited by fahtster, 12 December 2020 - 07:42 PM.

[fahtster]

3 hours later lol they’re tedious to make but I only have to do it like once a year



I get a ton of uses out of them though.. this guy is probably at mid-life and it’s not because they fail.. I just like new things



Pea+ laymixed today.. thinking it’s about to blow up.. they mature slow but any fruits that have matured fast, take a hit on potency IME



Other two pea+ tubs are starting to pin as well..
2nd laymix


Normal mixed with 1/4” cv top layer


I took that first ms PE tub to a second flush and it’s finally starting to pin



Faht

Edited by fahtster, 13 December 2020 - 07:10 PM.

[cujoloki]

I tried to make some lids today but its hard to get the band started with 3 layers of tyvek for me.

[fahtster]

Was one of them the fabric kind of tyvek? Yeah it’s a bit tough.. gotta push down on it pretty hard as you turn and apply even pressure on all sides of the ring.. much easier with one layer being fabric tyvek. If you cant get the fabric kind, 2 layers of postal might do the trick

Edited by fahtster, 13 December 2020 - 10:15 PM.

[cujoloki]

I have a shit ton of the house type. How applicable is this? What are your ideas on a 2ft by 8in cylinder tube impluse sealed out of tyvek for a bulk container?

Edited by cujoloki, 13 December 2020 - 11:50 PM.

[fahtster]

I’m not sure how thick the house tyvek is.. I use kite makers tyvek.. I’m not sure what you mean by bulk container.. like to sterilize lots of grain?

Faht

[cujoloki]

Wow i was really stoned last night. House wrap tyvek proved way too thick. As for the idea. I meant like a grow bag type of setup. I'll have to test it in the pc though and get a good seal setup going. Sorry for the vague/random comment.

[fahtster]

You’re all good!

[fahtster]

Pea+ pins



They start pinning in under 2 weeks but the PE part really kicks in and they mature sloooow (comparatively or I’m just watching em like a hawk lol) some of them have an awesome light powdery texture and color to em that I haven’t seen in pins before.. maybe because of how long they’re taking

Next round of pea+ jars gonna be shook tomorrow



Knocked up 4 qts with that be cake fruit clone yesterday.. excited for those

Faht

Edited by fahtster, 15 December 2020 - 03:57 AM.

[fahtster]

These pea+ tubs are looking a lot different than the first one I did.. I was expecting smaller fruits but these guys are looking chunky as all get out lol. Might be from the further isolation that happens when you do GLC jars



Ms PE 2nd flush.. looks like it’ll be decent for PE



Faht

Edited by fahtster, 16 December 2020 - 11:13 PM.

[fahtster]

Regular mixed pea+


Second laymixed bringing up the rear


Shook the big cake clone qts today and I have another set of ms pea+ qts @90% colonized.. learned a lot from these first ^^^^ tubs.. got a good plan going forward with tub set up

Second flush of that first ms PE tub.. glad I went for a 2nd


Faht

Edited by fahtster, 19 December 2020 - 01:43 AM.

[fahtster]

Alright party peeps.. since y’all have been so nice to let me post my pics and updates and in the spirit of the holiday season I have available 10 PE 2pk swabs for grabs.. I have two simple rules 1. Your profile has to be at least 2 months old and 2. Your safe addy has to be in the states. These are unbendable rules.

Just reply here 1-10 (just whatever the next number in the sequence is).. then pm me a https://privnote.com/ with your safe addy and I’ll get them out to you within the next couple weeks.. probably sooner than later.

I only have 10 available, so once those are spoken for, that’s it.. plz don’t ask after that, I simply don’t have them lol. Then I have to say no and you’ll feel rejected and I’ll feel bad because I had to make you feel rejected lol

Faht

Edited by fahtster, 20 December 2020 - 06:04 PM.

[jrh]

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