Last post #78 above and this post #79 are a complete brief summary of this entire thread. In conclusion, post #78 above continued here, ends here:
Below (1-6) from 1975 paper "Extraction and Identification of Clavine and Lysergic acid alkaloids from morning glory", see end of post #78 at top for latest "2016 attached 12 page paper", valuable morning glory study on LSH & other alkaloid levels found in morning glory from 3 different vendors, all levels very similar, collected from heavenly blue mg from 3 different regions located far apart.
The attached 2016 Polish morning glory study at end of post #78 (is somewhat similar to the 1975 study) except it shows penniclavine and LSH to be the 2 highest alkaloids, with the other alkaloids filling in the lower percentage.
1) elymoclavine = approx 17% of heavenly blue mg = 1957 paper from Yui Takeo showed that when animals were injected with elymoclavine, that they were stimulated MORE than when they were given LSD.
2) agroclavine = approx 25% of heavenly blue mg = 1957 paper from Yui Takeo showed than when animals were injected with agroclavine, that they were stimulated MORE than when they were given LSD.
3) chanoclavine = approx 7% of heavenly blue mg = 1957 paper from Yui Takeo showed that when animals were injected with chanoclavine, that they were stimulated just as much as when given LSD.
4) penniclavine = approx 25% plus of the heavenly blue mg = 1957 paper from Yui Takeo showed that when animals were injected with penniclavine, that they were stimulated just as much as when given LSD.
5) D-Lysergic acid hydroxyethylamide (LSH) = approx 25% plus of the heavenly blue mg. If the seeds are not frozen & stored properly, then over time LSH decomposes to LSA (Lysergic acid amide). So the seeds may contain a makeup of 1/2 LSH to 1/2 LSA a long while later, like retail rack seeds as the LSH decomposes over time.
6) Ergometrine = approx 5% of the heavenly blue mg.
From the 1957 paper:
All members of the excitor group produced in all test animals a syndrome of central sympathetic excitation and elicited a stimulation of spontaneous activity. In this group, elymoclavine, was the most potent stimulant and next come agroclavine, triseclavine, penniclavine, and LSD which are almost equipotent, as judged by the degree of symptoms exhibited in the same dose. The arousal effect of elymoclavine or agroclavine on reserpine-sedation was superior to that of LSD.
Animal experiments have shown that elymoclavine, lysergol, LSD and several other ergot alkaloids such as agroclavine, triseclavine, penniclavine, lysergine and lysergene have excitory effects on the central nervous system (Note 1: Yui & Takeo, 1957) as well as lysergic acid hydroxyethylamide (LSH) which also excites the central nervous system in animals (Note 2: Glasser, 1961).
The effects of agroclavine are similar to those of elymoclavine and LSD on rabbits (Yui & Takeo, 1957), indicating that the effect of agroclavine may well be psychoactive in humans as well. It also seems likely that agroclavine, triseclavine, penniclavine, lysergine and lysergene and lysergic acid hydroxyethylamide (LSH) will be psychoactive in humans.
LSH = D-Lysergic acid hydroxyethylamide in the seeds, we know it is similar to LAE-32 in TIHKAL, in which human experiments were done, at 1.5mg it was stimulating & "LSD like".
Glasser in 1961 noticed animals also became stimulated when injected with LSH. Dr. Glasser said some of the mice even stood on their hine legs and pressed on the noses of the mice in front of them, very peculiar.
Animal tests all point to LSH being an active psychedelic and it is indeed the closest thing to LSD found in nature, far closer than d-ergine. Owsley claims Hoffman himself told him that LAOH is very LSD-like.
It was Gröger who first discovered LSH in the seeds, published in his 1963 paper "Über das Vorkommen von Ergolinderivaten in Ipomoea-Arten". Later also Hofmann then extracted it from the seeds. It probably was in 1967, as Heim wrote in his work from August 1967 that Hofmann said he recently extracted it from the seeds (personal communication, as they knew each other very well).
LSD----------------------------------------CH2CH3-----CH2CH3.....chemical formula (C20 H25 N3 0)
LAE-32-----------------------------------------H------CH2CH3.....chemical formula (C18 H21 N3 0)
d-lysergic acid hydroxyethylamide-----------H---------CHOHCH3....chemical formula (C18 H21 N3 02)
Penniclavine-----------------------------------------------------chemical formula (C16 H18 N2 O2)
(1) The above experiments with mice, rabbits, cats and dogs who were injected with elymoclavine, agroclavine, chanoclavine alkaloids from morning glory can be found in "Neuropharmacological studies on a new series of ergot alkaloids" "Elymoclavine as a potent analeptic on reserpine-sedation" by tohoru Yui and Yuji Takeo, Hyg 911/LSD 494, Jap. J. Pharmacol. 7, 157 (1957). Jap. J. Pharmacol 7, 157-161 (1957).
(2) LSH experiments on animals: A. Glasser, Nature 189, 313 (1961)
(3) This is the paper that shows the alkaloid content of HBWR is vastly different from the alkaloid content of morning glory: Paulke A, Kremer C, Wunder C, Wurglics M, Schubert-Zsilavecz M, Toennes SW. Identification of legal highs—ergot alkaloid patterns in two Argyreia nervosa products. Forensic Sci Int. 2014;242:62–71.
No high levels of stimulating LSH, agroclavine, elymoclavine, chanoclavine, penniclavine found in HBWR seeds, only in morning glory seeds. A 2014 forensics paper from Paulke found no LSH in HBWR seeds, but only found LSA & iso-LSA (83-84%) & ergometrine (10-17%) & rest minimal: lysergol, elymoclavine & chanoclavine.
We know that MG has centuries of Shamanic use, while HBWR has no history of Shamanic use. HBWR only has history of medicinal use.
Sandgrease: "HBWR has more of a sedative effect compared to MG."
Nogal: "HBWR is more body related while MG seeds have effects more similar to LSD."
4) Aum_Shanti, 2019, "In fresher seeds there's mainly LSH (in relation to LSA). Only in old seeds, the LSA is dominant. This is because the fungi on the plant can only biosynthesize LSH (not LSA), and LSA is then a decomposition product of LSH over time. The fungi on the vines biosynthesize:
from tryptophan-->chanoclavine-->agroclavine-->elymoclavine-->lysergic acid-->ergometrine-->LSH, which then decomposes over time into LSA."
(5) Psychotomimetics of the Convolvulaceae pg 93: "This particular plant seems to have been more important to the Aztecs in divinity then Peyotl or Teonanacatl, two of their other classical sacred plants."
(6) Jonathan Ott "Pharmacotheon": "Ololiuhqui was far more prominent as an entheogen here in Mesoamerica than those mushrooms; the mushrooms are mentioned only here and there by a few competent chroniclers; yet almost an entire book was devoted to denouncing mainly the ololiuhqui idolatry. The annals of the Inquisition contain many times more autos de fe for ololiuhqui than for mushrooms."
(7) 2016 Polish morning glory study which finds 3x higher amounts of LSH in fresher MG seeds direct from grower/producer vs retail: hxxps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4830885/ LSA is a decomposition product of LSH over time (see attached pics from study).
2016 Polish MG study:
Alkaloids abundance in all 3 HB cultivars is comparable, with most significant difference for LSH (Lysergic acid hydroxyethylamide), which varies from 0.54 to 1.71 compound to IS ratio.
As has been demonstrated in this study, LSH is a labile compound, and therefore the variances in its concentration may be due to different age and storage conditions of the seeds rather than difference in plant metabolism. Indeed, seeds IT-HB2, which express highest concentration of LSH, were bought directly from the producer, whereas seeds IP-HB1 were purchased in retail stores.
 Researchers showed in 1961 that Claviceps paspali produces high amounts of LSH in culture: "Production of a new lysergic acid derivative (LSH or Lysergic acid hydroxyethylamide) by a strain of Claviceps paspali, Stevens & Hall".
Possible likely entheogen candidate used to serve hundreds of initiates at Eleusis in ancient Greece: this is where the Eleusian Mysteries were held, at the Eleusis Telesterion (initiation Hall for initiates...all men, women & slaves were invited) in ancient Greece.
Chemist Peter Webster wrote that fresh Greek claviceps paspali infected paspalum grass which grows adjacent to Eleusis in the famous Rarian Plane contains the exact same alkaloids as found in the fresh Aztec & Mayan morning glory. Albert Hofmann wrote that Claviceps paspali due to it's similar makeup to the Mexican morning glory could also have been the likely entheogen used at Eleusis to serve hundreds of people.
(9) Krystle Cole from the book "Lysergic":
"Isn't Ergot what Socrates used to take at Eleusis?" I thought it was kind of cool to be taking something that the founders of our democracy used to take, but that our current democracy has made illegal.
LSD chemist Todd Skinner replied "Yes". Todd had prepared 6 jugs of ergot wine and stored them for many years.
Krystle Cole's "ergot wine" experience (several pages long) in the book "Lysergic", reported that she saw constantly rotating holographic Sanskrit or Arabic & Zodiac symbols, floating in a circle around Todd's head.
(10) sample morning glory wine trip report from Erowid: Morning Glory & Alcohol by Psychopsilocybin:
I would only note that she or he should have most likely extracted the seeds from the start immediately into the wine instead of extracting into the water first...then adding to wine later, as this will cause the LSH to first decompose to LSA in neutral water or water that is not acidic.
Edited by tregar, 06 May 2021 - 02:56 PM.