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Cultivating wild strains project


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#21 cujoloki

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Posted 04 December 2020 - 02:23 AM

Now that I'll be fruiting in some time I need to get to work on this grow tent setup. I have a humidistat that I connected to a 3 disk fogger which is a pretty good unit. The fogger box I made has some flaws but pumps the fog into the tent well. I maintain 95% humidity fairly efficiently. The fog enters the highest vent. Ill probably panty hose the intake and 6"? outlet and call it good.

My next thought is to address the fae. I'm thinking that if the humidistat turns on every hour or so to adjust the humidity back up to 95% Then I'll be golden. I have to dial this in. If I have to much fae then I'll be wasting water in the humidity tank. I wonder if setting the threshold down to 10% until it kicks back in would work.

It's a 4x4x8 grow tent. I'm going to be making the shelves put of pvc. I think that will be cost effective amd easy to clean long term. I can hang bags from the pvc shelves with zip ties and i think it may do well.
Any thoughts are appreciated, thanks.

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#22 Auhron

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Posted 04 December 2020 - 03:04 AM

The Humidifier will kick on during/after each FAE ( assuming  you use some form of forced airflow). Recommended air exchanges for the species your working on (according to Stamets in Growing Gourmet and Medicinal Mushrooms) are 4x - 8x per hour.

With the volume of air in your tent you can probably aim for the lower end of that (unless your loading the crap outta your tent).  You are using a humidistat for control though, your water waste will be minimal.

Just my thoughts based primarily on what I've read, and watching the cycles on my own little fruiting chamber. Fan kicks on, and before it has finished its cycle my humidifier kicks on to bring the RH back up. Looking forward to seeing your set-up once you're fruiting all those mushrooms!



#23 cujoloki

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Posted 08 December 2020 - 01:44 PM

Update
Some bags showing signs of lions mane growth.
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Fruiting chamber almost setup! 16074528871671127953061.jpg

Reishi (from bought lc) I may try to fruit by just opening in the fruiting chamber. I will cover when sporulating if it is .16074529578841113342253.jpg

Edited by cujoloki, 08 December 2020 - 04:43 PM.

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#24 cujoloki

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Posted 12 December 2020 - 10:54 AM

Looks like the reishi test jar is going strong, and even forming primordia I think. It definitely thickened up.

Some of the grow bags are showing 20-50% colonization. Next week I should have some in the FC. The LC to bulk uses a lot of media. Some bags using 90cc. Only having 7 30cc syringes was a underestimate on the planning phase. Overall though having strong thick LC is what I learned so far. Don't waste your time being hasty. Let the LC grow out.

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Edited by cujoloki, 12 December 2020 - 11:26 AM.

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#25 cujoloki

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Posted 14 December 2020 - 05:42 PM

HeRicium bags are looking real good after 9 days. Some bags are 14 days and are ready for sure. Visible colonization is complete. Research proves that LM mycelium thickens up over time but will look wispy at full colonization. I moved 6 LM blocks in the FC today. Cutting a 3 in by 2 in X in the sides I want to fruit.

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The fruiting chamber currently has the 6 grow bags and the 2 reishi testing jars that are forming antlers off of popcorn/spent coffee grounds!? Pretty exciting stuff! I should see something special soon!

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From now on I will be using 60cc of LC for the 2.6 kg bags and 90cc for the bigger 4.8 kg ones. I think 60 is a bit of overkill for 2.6 but its what I got. I only have 30cc syringes. I looked for a thread to find the LC per pound of substrate formula to no avail.

I don't think many people do the LC to bags tbh. I find its fine if you make your sub a bit dry. I have made some almost 5 kg bags with hardwood and straw substrate that will fruit chicken of the woods in a week or so. LC is so fast but there are risks that what's in the LC isn't what you think. Of course due to this I have made many many petri, testing at almost every inoculation to be sure that what you i have is at least a viable fungus mycelium. Frankly, this being wild clone attempt there is no telling if this amateur really has succeeded until the fruits are in the FC.Cheers!

P.s. sorry for the sideways pics

Edited by cujoloki, 14 December 2020 - 05:56 PM.

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#26 Auhron

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Posted 15 December 2020 - 09:33 PM

So, if I understand you - you skipped doing any grain spawn for your Hericium Erinaceus, Just went straight LC into the fruiting substrate?  This approach is mentioned in McCoys Radical Mycology.  If I'm remembering correctly he said 60cc for 5lb of substrate so you're right on point.

The one drawback worth noting is that using grain spawn does add a fair bit of nutrition into the final substrate (depending on the type of grain / amount of spawn used), what did you use to supplement your hardwood?

 

Hoping those bags pump out some delicious pom-poms for you


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#27 cujoloki

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Posted 16 December 2020 - 09:39 AM

I used oat and white oak sawdust at 80/20 with one experimental uninnoculated biochar additive. I made a 150 gallon of biochar this summer. So a cup or so of that per bag. Hoping it's nutritious enough isn't going to make it so. I also am trying a straw sawdust mix out. Some of my huge 4.8 ish kg bags have that at 50% straw and 50% of the 80/20 oat sawdust. I ran out of sawdust on those. Not all of the bags have biochar so I can gauge effectiveness. I have 2 control bags with no experimental additives as well. 1 bag of the charcoal supplemented is control and not inoculated so that I can see how it does on its own.

#28 rockyfungus

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Posted 16 December 2020 - 10:07 AM

I've always used supplemented hardwood. Either bran or soybean hulls. I know oysters do fine on almost any medium, but lions mane seems a bit more finicky.

Curious to watch this biochar experiment. Makes me want to supplement some compost in the spring instead of bran.


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#29 Auhron

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Posted 16 December 2020 - 12:14 PM

Cool. I'm lookin forward to see your results!


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#30 cujoloki

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Posted 16 December 2020 - 12:14 PM

IM curious to find out what limits ill run into supplementing a woodlovers substrate. First off whats more nutritious than wood for a woodlover? Well popcorn/grain spawn is as most know the nutrition boost needed in bag grows. Well if the LC i use has a hardwood starch base and a sugar content then isnt that almost as good? The Life endured by colonizing the grain and having a base from which to colonize is the major benefit as well as homogony for the substrates sake. Wouldnt a LC thats allowed to completely colonize be almost as good? Maybe even betteR? Fine tuning will be needed. 

Also another point, adding more grain to the hardwood substrate.  Would this work? Should i add some cracked corn or WBS or pearled barley etc to hardwood sawdust and oats? The different cooking times would maybe make a mess and a technique would need to be developed. I may be satisfied with the original results though. Or? Would some kind of chemical additive be a better option. Maybe a  LC with more starch content would be a better fit. A suped up PDA without the A. 



#31 cujoloki

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Posted 29 December 2020 - 09:51 AM

Long overdue update. Incoming is a host of pics from the FC. No pom poms yet but the reishi is climbing. I have another jar and 3 bags of in vitro reishi going as well as some lions mane jars to fruit from the top. The LM bags are either slow or a pain to guess where it's going to fruit. I have had to close an X with painters tape and cut in a different spot a week later because the block shrank and wanted to fruit on bottom. I have some definite fruiting happening though. Some of the blocks shrank so starts are happening all over I think. One bag has completely pissed up and i believe has fruits inside the bag. All in all this LM takes 2 weeks to start fruiting once introduced to FC. A few blocks i had should have been put in earlier. The species doesnt like to show consolidation on sawdust or it needed 4 weeks to actually colonize well. Im weighing my conclusions. The LC seems to be taking a long time to do its thing. We will see in another week or two.

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#32 cujoloki

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Posted 29 December 2020 - 07:42 PM

On one of the pictures I can clearly see poms starting inside the bag. Should I open the bags more?

#33 cujoloki

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Posted 04 January 2021 - 12:45 PM

Well, the reishi look good. I haven't had any poms poms yet from my lions mane. Also I have found a black ink substance around the openings. I may need help identifying the issue here. Not all of the bags have it but I think it is black mold. Frustrating as hell. It smells nice like lobster in the tent but no poms. Included are pics of the above stated. One bag I opened more and i found a lot if spots that are black and inky. I'm probably going to toss them all. I don't want to but I bet it will just sporulate and spread. Since the lions mane aren't vigorous after a month of being in the FC I must face the fact that LC spawn run went bad and or I waited too long. Not really sure but I guess I'll have to wait until round 2 to get lions mane. The chicken of the woods round of bags are running well. And i think I'll introduce them to FC in a week or two.
All in all i think iv learned that lions mane doesnt like to be LC spawned. I should have went popcorn to supp sawdust. Going to leave out the charcoal additive. The reishi seem to enjoy it as they crawl out of the popcorn jars. The black mold is only on the LM. This leads me to 1 of 2 conclusions. Either it was in the LC the whole time or my fogger brought it in. I remember having the black ink pop up in some ketchup cups which I tossed right away. I'm a little discouraged at the moment because of this but I'll try to isolate the problem and work on it from there. I am leaning towards environmental contamination but the reishi look healthy to me and have been open air for 3 weeks now.
Thanks for reading and any and all help is appreciated.

The first 6 pics are what I'm throwing away. I decided as I looked. They are All going outside to maybe fruit later. I have 4 LM bags that are bad and 4 that have no black yet. The last pic is the reishi that are looking fine.

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#34 Arathu

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Posted 04 January 2021 - 08:20 PM

I had LM bags with fully colonized blocks do that too.....I didn't Identify what happened as they began to smell pretty bad afterwards. Compost heap material here...

 

Looked very close to if not just like that actually. Something likes Lions Mane more than we do. I put some to logs this year and will be making plugs this coming season. 

 

My Reishi logs are insane but no fruits yet....

 

You ain't alone.......

 

A


Edited by Arathu, 04 January 2021 - 08:32 PM.

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#35 cujoloki

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Posted 05 January 2021 - 12:05 PM

Thank you Arathu! Thank you for your advice and commenting. Im trying to grow these for my familys health and i need to make this work somehow. My RH is set to 95%. Im thinking i may lower the RH to 85% due to this black monster eating my poms. Your comment eased my mind a bit. Almost shed a tear throwing those out yesterday. The tent smells like lobster heaven today as i mopped it. This being my first grow i have alot of hurdles to overcome as well as flaws in my technique that need to be corrected for faster spawn runs which may help. LIons mane is a challenge for me to recognize when spawn run is done and when fruiting starts. Should i wait for primordia? 



#36 cujoloki

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Posted 12 January 2021 - 03:10 PM

Lions mane still is taking its time. No poms yet but i have decided to boost my patience threshold so to speak as well as expand my species collection. Bear with me as i try to upgrade the quality of my additions to this thread!

A month ago i cloned a Portobello and some Turkey Tail fluff and put it to Agar.
 

 

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 A total of 6 for the 'ports' and 4 for the 'Turkeys' if i remember correctly. 2 weeks later i transferred 'ports' from 2 of the "cups" which made the grade to make 4 cups which have filled in; the 'turks' (Trametes versicolor I wild Harvest and made tea with which had been sitting on a shelf for months all ready for depositing) . With those i have made a liquid slurry with sterile syringes and distilled water. I put 10cc or so of distilled water in each cup and scraped with the syringe tip until the surface was completely scraped. Then i sucked up what i could and put it to popcorn. Here are pics of the scraped cups
 

 

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These jars of P(ports) and TT(Turkeytails) will be used for g2g and be spawned to my home-sawed hardwood mixture and chopped straw mixture respectively. 
 

 

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Earlier this week, i used a PC tek to hydrate popcorn and loaded 13 qt. of popcorn for future LM  to grain as well as more Reishi. The lids on the jars are 1 layer of precisely cut house wrap tyvek that seems to be working. I use painters tape to seal the inoculation site. And all this is done in a SAB that i have been using with great (haha) success so far. Later i will use the LC of the LM and reishi i have to these qts. 
 

 

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As far as expanding would go i want to also harbor the cremini or button mushrooms next so in a few days i will clone on of those. I also want to try to procure a chanterelle from a beautiful spot i know of around here that produces them. That would be a delight and i will have to research those and see how they grow. I hope they aren't parasitic (symbiotic or needing a host tree??) or need a mutual partner to grow as i sit here and think about it. I have found them by pines and they are great with alot of dishes. From memory they have to be wood lovers.   

Chick of the woods are looking great! Being a month old and some 4 weeks they look almost completely colonized! There is one Lions mane still in there. I will be putting them into the FC along with everything else and hoping for the best @ 95% Rh and around 65-70 Degrees. Which is the best i can accomplish at the moment.

 

 

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Drastic improvements to my fogger which had a horrible design from the beginning. I opted for a top mounted fan. One of the previous 2 fans that were mounted on the side of the main fogger reservoir died due to too much moisture. These were pc fans and i have a waterproof fan coming soon that will last longer. The problem was too much backflow/crossflow with 2 fans. After the fans discharged the fog, the fog would equilibrate into the side chamber making a pool that would fill up over time. I fixed this after noticing the 1 of the fans seized up. Live and learn i guess. The original idea was to have great airflow but the design was horrible in retrospect and the house tyvek cover i siliconed  and tek screwed on the outside of the side tub wouldn't let enough airflow through to push a gnat. Tek screws were a bad idea as well :smh:
 

 

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The fc looks good and still smells of lobster faintly. Some of the Lions Mane bags are progressing i think ( :biggrin:).

 

 

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One of the bags has a discoloration to the puffs that appeared Dont know if i should worry yet.
 

 

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Im thinking of harvesting these reishi and tincturing these right away, but i want to let them grow out as much as they can. The 3rd reishi jar is puffing up well! Should i harvest the 2? I haven't seen a spore load as of yet. I think they are still immature but the health benefits are in the mycelium more than the fruit i remember. The maturity wouldn't matter much in this case. Im interested if they will spore from the popcorn though.  :ohmy: (Insert scratching head emoji)
 

 

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Hopefully ill have something more delicious to look at soon! Thanks for stopping by!


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#37 cujoloki

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Posted 17 January 2021 - 12:20 PM



Harvested 2 reishi jars today! Making tea with the fruits. Going to dry out and tincture the myceliated grain as well as make some tea with it! Super happy about this. The whole house smells like reishi right now!



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#38 rockyfungus

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Posted 22 January 2021 - 12:09 AM

How are your lions mane's going?

I made 3 bags of master's mix on 12/18. About 20 days in they started showing teeth

Something tells me those lion manes are contaminated.



#39 cujoloki

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Posted 22 January 2021 - 06:32 PM

Those all were thrown out except 1. They weren't doing well enough i thought. Hers is the last LM mohican for the time being. Im not sure what to do with this bag except wait at the moment.

 

 

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The reishi is doing fine. The FC tent looks mighty empty right now

 

 

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The chicken of the woods bags are becoming more and more colonized. I removed them from the bin incubator i had them resting in because i thought it wasn't allowing for enough airflow. I am leaning away from LC to bulk from now on due to the variables i am experiencing.

 

 

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My grain jars were very successful though.

 

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Yesterday i G2G'd 1 jar of Turkey Tail and 1 jar of Lions mane. I made 8 qts. from 1 qt. with the lions mane and i made 4 qts from the 1 qt of Turkey tails. Had a little extra Turkeytail spawn left there.

 

 

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The Lions mane and Turkey tails colonized in a week. The Lions mane was nocced up with old LC from a jar that was 2 months old left in the incubator. The turkey tails was a scraping from agar cups and distilled water injections.

 

Portobellos arent doing well though. I havent noticed any growth on the portobello scrape injected popcorn so far.

 

 

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They were done the same way as the turkey tails so maybe the culture itself wasnt strong enough yet.

Made up 6 2kg sawdust supplemented bran today. Going to spawn 2 qts of turkey tails that are done enough and 1 qt of lions mane popcorn spawn as soon as they cool off. Ill be doing 1qt to 1 2kg bag. I can only fit 2 of the bags into my old school huge pressure cooker. Ill be trying to go for a 4 hour sterilization this time. We will see if the bags melt. 

 

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#40 cujoloki

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Posted 24 January 2021 - 09:19 PM

Hi folks. Im making some definite progress this time around with the grain spawn. TT qts' and LM qts are colonizing well. Im going to end up having 8 LM qts. and 3 more TT qts. done in a week. The TT are fairly more aggressive due to the amount of spawn i used on their jars. In the corner of the picture you can also see one of the 3 bags I have spawned so far. 

 

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I made 3 2.5kg bags out of the first popcorn spawn run of scrapings of TT and the last of the LC of the LM. They will colonize out of the bin that i have been using as a dust barrier that may have been stifling airflow to the colonizing bulk bags i stored in there. Trying to tighten up ship where i can and that involves taking all my bulk bags out of the bin and making room on a shelf for them (and on top of the bin itself) for the time being. The closet and bin top is the only place i have for them at the moment until i build another wall mounted shelf. Contamination probability rises with this i know so i will be wiping any and all bags down heavily before introduction into the FC.

 

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I went to the neighbors and cut some more wood yesterday. I use canola oil in my saw and i collected a tarp worth of sawdust and brought it home to process into more bulk bags for TT and LM etc. My soaking method is getting easier. I now preload the sawdust into bags and soak overnight with boiling water. I will then load into spawn bags mixed with some old fashioned oats and PC for 3.5 hours. 2 bags of this sawdust gives me 5 - 2 kg bulk bags to work with. I should have 10 out of this round.

 

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I want to fruit this Chicken of the woods so bad! So i have these 6 week old chicken of the woods bulk bags that were made from straw and sawdust and innoculated with the Chicken of the woods culture i made from a clone. I believe i have what are pins showing on one bag. Now for the big question. Do i cut an X over it? Which hasnt worked for me so far either due to lack of efficiency in the FC or bad method and not having blocks that are healthy enough etc. Or.... Do i cut the top open and let it go? I seem to have an issue with top cutting open on my reishi block. It likes to pool in the bottom and i go in and pour the standing water out of it every cleaning cycle. So cutting the top maybe nice and effective but i may need to also cut relief/ drain holes into the bottom to let the water drip out naturally. On the bags i have overkilled the sealing of the inoculation points and have a bag or 2 that i may have recycled for curiosity sake.. Whats the opinion on these bags? They look fully colonized to me.

 

This bag has what looks like pins

 

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Some fully colonized bags

 

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The rest of December's bulk bags. 6 chicken of the woods and 1 lions mane that is sawdust and coir and 6 weeks old. Im going to let this one LM turn completely white before i do anything this time.. The recent bags may be done before it is.. 

 

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