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Cultivating wild strains project


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#41 cujoloki

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Posted 24 January 2021 - 10:55 PM

My agar work has been undocumented for some time. lets remedy that. First off i have the agaricus bisporus clones that are doing something. They seem to be mycelium and not yeast/bacterial so far. They were taken from a young "button". They are around 2 weeks old.

 

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The Original Turkey tail clone i started from "fluff"  which is blended up Trametes Versicolor; from a wild specimen. This has been A joy to grow. Compared to agaricus which may like other supplements in their agar TT has grown beautifully.

 

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This is a sample of TT from the grain spawn jars. I want to see if this new 4% dextrose and corn starch agar makes any difference in the way TT looks on agar.. 

 

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This is the original Agaricaus Bisporus clone taken from a "portobello". There is a strand of mycelium that im wanting to transfer and wonder if it will be worth it. 

 

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Two t4 lions manes that i have kept for over 2 months. They have fluffed up alot, have had transfers taken out of them, and are on a hardwood water base agar with 2% dextrose. I may transfer from those... I dont really know why i keep them.

 

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The scraping agars have been recovering well. They all have wispy growth forming again. The one cup that looks different is a TT that i didnt harvest mycelium from. It looks nice in the cup and has umbrella looking mycelial growth.

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A close up of the Agaricus scraping forming another light mat.

 

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Overkill amount of transfers from the latest Agaricus clone on the new agar. I have alot of redundancies at this point.

 

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Some grain samples placed on agar from the latest Grain to bulk trasfer that were left in the jar. They are Lions mane and im hoping they like the 4% and corn starch agar iv made them better than all the others.

 

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#42 cujoloki

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Posted 25 January 2021 - 08:25 AM

I found a research paper last night on fruiting Laetiporus Sulph.. https://www.ncbi.nlm...cles/PMC3599174

 I'll proceed to cold shock these blocks either today or tomorrow and proceed with fruiting. 



#43 Auhron

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Posted 25 January 2021 - 09:22 AM

I am interested in seeing how your Agaricus cultures will turn out. I have had ZERO success with my attempts with Agaricus Bisporus.

I've tried to clone tissue to agar, grain, and LC's to no avail. I either get no growth at all, or I get epic bacterial cultures.

I've tried Spores to agar multiple times, germination has never been a problem, but the mycelium grows out from the germination point about 2cm then stalls out. Transfers have never recovered.

 

Looking forward to seeing your Chicken of the Woods too!



#44 cujoloki

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Posted 25 January 2021 - 01:57 PM

I read earlier today that they are secondary decomposers. meaning that they love to be the sloppy seconds of the microbial world. I think they are doing well and colonizing the popcorn from my injection so far. Not as fast as the Turkey tails for sure. The G2G for the TT is almost done after 4 days. Too bad they arent more delicious. Ill welcome the medicine though.
Im currently researching the web about Agaricus bisporus and what is best for bulk. Looks like trays with mixed supplements and casings are they best bet. But... I want to try peroxide saturation like in stamets' book for straw. Ill look it up again when i do the procedure. Ill use bags for now and maybe case the top. After an initial trial run of course ill see if i can get these going.



#45 cujoloki

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Posted 25 January 2021 - 02:25 PM

Here is a site that is a pdf for growing Agaricus Bisporus. This was publishied in 2018 i think. https://www.ijcmas.c...etam, et al.pdf



#46 Auhron

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Posted 25 January 2021 - 03:34 PM

Thanks for the link. I'll have to try germinating some spores for the Agaricus Bisporus on PDA, I've used MEA on my previous agar attempts, maybe that will help.  I really want to start them from scratch like I did with my first oysters (as opposed to purchasing a live culture).


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#47 cujoloki

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Posted 26 January 2021 - 10:29 PM

Agaricus is showing growth at 17 days old now. I should look this up and check it visually to see if it matches other agaricus spawn. It's very thin and very wispy I'm not sure if a picture will capture it well. Also not sure if I can G2G this yet or spawn to things. Maybe it will expand some more in a few days


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This spawn needs to hurry up and do its thing.

6 total 2.5kg bags done so far. 8 more on the way. Tally is 4 Trametes Versicolor and 2 Hericium E. I'll be adding 7 more H.E. soon and another turkey tail.

Did I mention these turkey tails grow like crazy?
The first 2 bags I PC this time got a bit hot. I quickly taped the cracks in the bottom. 4 hour PC time needs 3 to 4 quarts and foil protection to be safe.


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#48 cujoloki

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Posted 26 January 2021 - 11:25 PM

On second thought I may throw those agaricus jars away after the latest best clones have shown some great growth. These 2 t2 are 6 days old and loving the new corn
PC water (pretty much corn starch) 4% corn syrup agar recipe. Those are the best views I can get. This mycelium is whiter and definitely more active. I hope this grows out to a nice specimen.


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#49 cujoloki

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Posted 06 February 2021 - 06:55 PM

Alright, Im back! After a week or so I've built up enough to report about that i think is substantial enough to get the old PC (personal computer) out. The last reishi jar was done fruiting i cut the top off and put the contents in the dehydrator today for processing. Actually retrieving the reishi was a chore by the way. I am never doing the reishi like this again. Too much trouble; when it grows a long time there is a leather like consistency that develops. The reishi bag looks like it is forming antlers! It looks lonely in there after i had to toss the Chicken of the woods out yesterday. Hay was a bad mistake, in my honest opinion, it was the vector of contamination that caused some aspergillus mucorales I believe. They got tossed and since the FC has been decontaminated.
 

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The Lions mane and Turkey Tail bags are doing great. The Turkey tails Have all but completely expanded and will be going in the fruiting chamber so that mr reishi bag isnt lonely anymore. 

 

20210206_160350.jpg   I have 4 more bags that look exactly like this. 





The 6 (lost a pic i guess only 5) lions mane that are showing signs of wanting to expand. Some clusters have formed as well which is a good sign for me. Some of the bags were spawned on the 23rd which will be 2 weeks tomorrow. These spawn jars were on the wet side of things so if they colonize completely ill be surprised even though they show growth signs as of today.

 

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The bags in the top of the closet are sitting at a nice and toasty outside temp of 79.6 ish degrees. I think they like having more open air as well as being close to each other. I find if i turn a bag around where they touch colonizes faster and i will rotate bags so that even colonization occurs eventually.  

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I have recently upgraded (i think) the popcorn spawn i use. After using a recipe i found which stated that a 1 hour PC of popcorn is sufficient i have found using my equipment that the grain is holding too much H2O this way.I followed up with a 2 prong approach which involoved adding one third cup appx of Verm to my jars as well as  PC'ing them for only 50 minutes. Poured out hot, then let to dry overnight after straining on a towel. This has proved successful in the case of using my Liquid Culture for Lions mane. this is being spawned at 6 days old.

 

Pre-Shake

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Post-Shake

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So successful that i can expand to 6 more qts and see how they like it. I think that my 'new grain' are still viable for agar transfers and have enough moisture for 'non liquid expansion' and this will prove ... that. 

 

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I expanded the qt to the 6 other qts. after i took this picture and labeled them with todays date and LM as a specie mark like i always do. Thanks for reading and have a great journey with your myco friends!

 

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#50 cujoloki

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Posted 21 February 2021 - 10:57 AM

Time to write an update. Those 6 qts. where i left off were spawned into 6 2.2 kg bags. Of all of those bags none have survived. The spawn was narrowed down as the vector. It noticed the grain spawn was wet and the sample was corrupted with yeast i believe. Anyway here's some pics of the bags that are going into compost today. These are about a month old. Some was spawned on the 23rd of Jan. Some before even. Spawn should never take this long to colonize. 2 weeks is going to be my new max for this species as anything else is just wasting hope and energy. I held onto them for so long i think because it was 40 degrees in the house for a week. The latest 6 bags , I will show you soon, have almost fully colonized in 10 days in this 40 degrees environment. That tells me that these bags are trash/compost.

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You can see the grains stalled before fully colonizing the substrate. As well the substrate isn't solid so to speak and has some give when pressed denoting that the sawdust isnt fully colonized. 
For some kicks i mixed 2 of the 6 bags last night to see if i could and they mixed well but these are going to the compost with the others. They aren't going to make anything close to what i want being all messed up with competitor organisms. Some of the bags have leaks as well. I need to be more careful spawning in the glove box and smooth some of the ribs from the lid that i think poked a hole or two in the spawn bags.

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mixed bags of wasted bulk spawn =D

 

 

The Lions mane that almost completely colonized in 10 days of super cold weather is making me happy. I have 5 bags of this because 1 succumbed to infection from a tear in the bag (curses glove box rib spike things). They look great to me. This is from the culture that was in the fridge i should add. The one i stored 2 months ago. I knocked up 6 more jars at the time i wrote the last post in this thread due to a feeling that the spawn wasn't going so well. Im glad i did that. Im also glad i made the change to verm + corn for my grain jars. That may have been contributing to the failures due to the moisture making the environment more suitable for yeasts/bacteria. None of my verm + corn jars have shown any signs of bacteria etc since! Now to tighten up the other areas im lacking in. The glove box is one area. I need to smooth out those dam ribs so i can spawn bags without popping them in the box ffs. Another is my FAe in the tent i want to address. I need another waterproof fan i guess and another filter box. I may move my humidifier into the FC so i dont have to buy another ducting setup and make an outside filter box for the FAE. It would only take a timer and this new fan and i think i can make it work with the humidistat i already have etc..

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The reishi are growing soo slow! I see a thick antler coming up for the time being. The archives weren't wrong when saying this mushroom takes a long time to grow. The cold didn't help much either. It got down to 9 Degrees Celsius in the FC over the last week or two. Another reason I haven't updated is that growth has slown down in this time.

 

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I have 4 bags of turkey tails in the fruiting chamber! They could not get more colonized. No primordia of fruits so far. I attribute that to the cold weather as they have been in the FC for about a week, but they smell and look pretty! 

 

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Note about picture uploading. These particular set of pictures i had to upload 3 times. I found that this format will rotate your picture for some reason and editing them in photos and rotating them the way the format does then redoing the edit and saving it right side up fixed the incorrect rotation this format does to pictures. Good luck to all your grows mycotopians!

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#51 cujoloki

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Posted 27 February 2021 - 09:07 PM

Some great success to report. All of my Turkey tail bags are starting to develop growth on the tops of the bags. It is all grey looking and will more than likely be grey like the phenotype of the wild harvested Trametes Versicolor i found last year. Hoping for some beautiful florets!

 

 

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I have 5 Wild Lions mane bags in the FC with x's cut on the tops. Going to be a good grow this time around i can feel it.

 

 

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Reishi antlers going strong! I want to do more of these beautiful babies.

 

 

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I want to have a storage system for specimens and have been looking into the glass vials i see some of the professionals use here. Ill look into this as i may have to procure a device for snapping the lids on the vials. They have sealing injection ports on the vials and i think it is mycelium stored in distilled water that was grown on nutrient deficient agar. I love the information this community has to offer.


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#52 rockyfungus

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Posted 27 February 2021 - 10:07 PM

Those are lions mane bags, you're gonna get it this time!


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#53 EYMAIOS

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Posted 28 February 2021 - 04:37 AM

.

Great project!

I have procured a Lions mane LC to try growing, so your info is precious.
By the way: what is "FAA biofilm"?

Carry on Bro...
 


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#54 cujoloki

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Posted 28 February 2021 - 06:12 PM

.
Great project!
I have procured a Lions mane LC to try growing, so your info is precious.
By the way: what is "FAA biofilm"?

Carry on Bro...


Fish Amino Acids. Fermented organic fish fertilizer. Pretty much 50/50 brown sugar to chopped fresh fish fermented for 3 months then strained.
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#55 cujoloki

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Posted 28 February 2021 - 06:14 PM

That was a total failure in the format i used it for anyway. Maybe with a more sterile method later on down the line I will try for a nitrogen boost.

#56 hyphaenation

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Posted 28 February 2021 - 06:14 PM

Great thread!

:thumbs_up:   KNF



#57 scott_1971_h

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Posted 28 February 2021 - 07:48 PM

KNF?



#58 cujoloki

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Posted 28 February 2021 - 10:40 PM

KNF?

 That would be Korean Natural Farming. A pretty deep subject. Fascinating really. Definitely a growing system for the everyday man/woman who lives on a budget and cares about building soil. Everything you use in KNF is also edible. I wouldnt eat IMO though... I havent tried the FAA yet although i may try to make my own fish sauce one day. 


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#59 EYMAIOS

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Posted 01 March 2021 - 03:05 AM

 

.
Great project!
I have procured a Lions mane LC to try growing, so your info is precious.
By the way: what is "FAA biofilm"?

Carry on Bro...


Fish Amino Acids. Fermented organic fish fertilizer. Pretty much 50/50 brown sugar to chopped fresh fish fermented for 3 months then strained.

 

.
Imagine an ancient gourmet product under a modern terminology: FAA=GARUM

.

Garum was a fermented fish sauce used as a condiment in the cuisines of Phoenicia, ancient Greece, Rome, Carthage and later Byzantium.

Although garum enjoyed its greatest popularity in the Western Mediterranean and the Roman world, it was earlier introduced by the Greeks.


Edited by EYMAIOS, 01 March 2021 - 03:06 AM.

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#60 cujoloki

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Posted 07 March 2021 - 12:12 AM

.Great project!I have procured a Lions mane LC to try growing, so your info is precious.
By the way: what is "FAA biofilm"?
Carry on Bro...


Fish Amino Acids. Fermented organic fish fertilizer. Pretty much 50/50 brown sugar to chopped fresh fish fermented for 3 months then strained.
.Imagine an ancient gourmet product under a modern terminology: FAA=GARUM
.
Garum was a fermented fish sauce used as a condiment in the cuisines of Phoenicia, ancient Greece, Rome, Carthage and later Byzantium.
Although garum enjoyed its greatest popularity in the Western Mediterranean and the Roman world, it was earlier introduced by the Greeks.

I should research this garum. How is it made? I have been very busy lately, sorry this communicae has taken a week to receive a response.




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