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Spore printing wild mushrooms


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#1 Xeric

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Posted 20 November 2020 - 06:21 PM

Curious about your experiences making prints from outdoor collected wild mushrooms. Any advice is appreciated! Thanks 4F82A8AC-2B6A-42A1-8AC4-3B1AF955D8E8.jpeg 026EE55F-C0E9-4D3A-A800-0659221352C7.jpeg
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#2 rockyfungus

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Posted 21 November 2020 - 08:17 AM

It appears you have printed them, what info are you seeking?


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#3 Boebs

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Posted 21 November 2020 - 10:01 AM

Hows your agar game?
Wild prints are usually full of contaminants.
Make 4-5 agar plates and take transfers from the least contaminated.
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#4 Xeric

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Posted 21 November 2020 - 10:56 AM

Thanks for your response rockyfungus, I was wanting to know if it has been your experience that prints from wild mushrooms are loaded with contaminants and if you’ve found any effective disinfectant methods to clean caps before printing.

Boebs, thank you for your insight and approach, this is the kind of feedback I am looking for!
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#5 rockyfungus

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Posted 21 November 2020 - 12:53 PM

What Boebs said is the ticket. They are inherently contaminated and will need 3-5 transfers in my experience to prove they are clean and just mycellium.

I've heard dipping in bleach, but never tried it as it will weaken the mycellium.


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#6 Boebs

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Posted 22 November 2020 - 02:42 AM

Ive have great luck doing this,

Collecting feild specimen right before the veil breaks,
Wiping them down with 91% alcohol,
Placing them into a small jelly jar to spore that is sterile.
All out in the field.
Ive made spore syringes out of these and had great results.

How ever those prints you have will be far from clean.
And 2-5 transfers on agar plates will be needed.
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#7 Arathu

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Posted 22 November 2020 - 10:33 AM

And just like that......set about cleaning those up on agar and do as you will....

 

Once I had my first prints and basic agar skills it was over from there......

 

Nice!

 

Welcome to Mycotopia.....

 

A


Edited by Arathu, 22 November 2020 - 10:34 AM.

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#8 rockyfungus

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Posted 22 November 2020 - 11:33 AM

Ive have great luck doing this,

Wiping them down with 91% alcohol,
Placing them into a small jelly jar to spore that is sterile.

Wiping the external surface with alcohol? Or even close at junction of cap/stem?

Do you go from jelly jar to syringe? Or just sterile water and swab?
 


Edited by rockyfungus, 22 November 2020 - 11:34 AM.

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#9 Boebs

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Posted 22 November 2020 - 05:28 PM

What i used to do was have a small jelly jar with a paper towel inside wet with alchohol.

Once i found a mushroom with the veil just about to break, i would cut it off ss close to the veil as i could, use the paper towle soaked with alcohol to clean the cap as gently/ throughly as possible, also use the same paper towle to clean my hands/knife and jar.
Once the alcohol was dried, then id place the cap inside and close the lid.
Hide it by a tree for a while.
Then once a nice print was visible, id open the jar dump out the cap.

Later at my work station, use a clean framing nail to pop a hole, and inject a little water from my steril syringe, use the tip of the needle to scrape the spores loose. And suck it back up:)

#10 rockyfungus

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Posted 23 November 2020 - 01:44 PM

How sterile is the spore print when you are injecting into the gills? I've been considering trying something like that to make a spore syringe, but figured I'd still have to clean on agar.


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#11 Boebs

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Posted 23 November 2020 - 09:28 PM

Ive gone right to brf cakes with it and usally get 7+ out of 10 jars to take without contamination.

Before crypto.. i nvr bought spores online..

So i had to figure out how to get prints clean enough..
And didnt have the knowledge to use agar.. didn't even know that was a thing at the time lol...

Ive been meaning to do a picture write up about it...
Maybe il have to gather up my stuff and make it happen
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#12 Myc

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Posted 23 November 2020 - 10:14 PM

Xeric,

So far, it looks like you've done a great job.

Agar skills are a must and they're not that hard to accomplish. A simple glovebox, some 1/2-pint jars, a pressure cooker, and you're well on your way.

Depending upon how you approach and handle the samples.......they might not be as dirty as you think.

 

I've handled a number of wild specimens over the years and quick transfers are the best method for clean culture.


Edited by Myc, 23 November 2020 - 10:22 PM.

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#13 Xeric

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Posted 23 November 2020 - 10:52 PM

Thanks Myc, my plan is just to drop a few spores into several 4 oz jars half full of popcorn inside the SAB then transfer healthy growth to cardboard and wood chips. Got this idea from WayLitJim’s old post. He has mastered the wood lovers!

Edited by Xeric, 23 November 2020 - 10:52 PM.

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#14 Myc

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Posted 24 November 2020 - 12:48 AM

You can't go wrong by following WLJ's work.

Best of luck!


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#15 Arathu

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Posted 24 November 2020 - 06:46 PM

You can't go wrong by following WLJ's work.

Best of luck!

Word.....

 

The experience gained by following his work is all that you need to know too....

 

A


Edited by Arathu, 24 November 2020 - 06:53 PM.

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#16 Myc

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Posted 24 November 2020 - 10:52 PM

I also recommend that you look into work by Hyphaenation.

He is yet another very humble, yet genius cultivator of most all things. I linked his profile so you can search all of his work - both myco and canna. Seriously fun stuff to be had there - with the cardboard tek and more............


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#17 Arathu

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Posted 25 November 2020 - 06:51 AM

Don't forget to check out the work by this guy ^^^^^ Myc too......ALL THREE, Waylit, Hyphae, and Myc, of the above turned me into a forever woodlover.......

 

You're in a very good place......

 

A


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#18 FunG

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Posted 25 November 2020 - 10:17 AM

I just wanted to mention that rubbing alcohol does not kill mold spores.

You'll want hydrogen peroxcide to kill mold spores. :)

I was disinfecting using alcohol for 16 friggin years before I found that out.




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