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H. Erinaceus, my 2nd colony


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#21 Auhron

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Posted 12 January 2021 - 04:31 AM

NOoooo !!  :sad:  Hopefully those other bags fruit 



#22 rockyfungus

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Posted 12 January 2021 - 10:08 AM

Hopefully I can learn from whatever happened here. Spawning 2 more KO bags today. Currently PCing masters mix, and I have a bag of masters mix that I sterilized a month ago. The month old bag I'm checking for viability of previous sterilized media, may sterilize another bag and leave out till it's obviously contaminated to get a sense of how well a 2 hour PC cycle really works for wood. (Stamet claims 4hrs minimum, with additives.)


abortedoysters.JPG

Lmspining.JPG


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#23 rockyfungus

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Posted 13 January 2021 - 10:55 AM

Now my Lions Mane is super unhappy. Starting to bruise. Really struggling this winter with my gourmets. I need to design a better fruiting chamber, but life is getting too busy. 

 

BruisingLM.JPG

Abortkings.JPG

Do I even want to open these 5 other lions manes block and 4 kings. Bit disheartening considering I've shared so many of these fruits with friends/colleagues.


Edited by rockyfungus, 13 January 2021 - 10:57 AM.


#24 rockyfungus

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Posted 18 January 2021 - 10:38 AM

Super pumped! After two years of trying to print Lions Mane, it finally happened.

It should not have been this difficult!

 

A very mature specimen was stuck in a SAB with armholes covered, tinfoil was literally wrapped around the fruit. A bowl of water was left at bottom of SAB for 3 days and no spores were visible on foil.
Yesterday the SAB was misted with H2O, and I guess getting the humidity up triggered sporulation! The foil was covered in dense dense white spores. I am currently printing again hoping to get a few more prints.

Now on to trying to swab or print some King Oysters.


Edited by rockyfungus, 18 January 2021 - 10:46 AM.

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#25 Arathu

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Posted 18 January 2021 - 07:11 PM

They look like they dried out.....man those were looking SWEET rocky.......

 

EDIT: maybe just old if they sporulated IDK

 

I agree you need to set up a nice fruiting chamber for those blocks.....

 

Good work man!

 

A


Edited by Arathu, 18 January 2021 - 07:12 PM.


#26 rockyfungus

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Posted 18 January 2021 - 07:45 PM

Decided to try something different with a lions mane block. Flipped it over instead of leaving upright and cut V's instead of X's. This should keep humidity in the bag a bit longer, and then the fruits can push through the flap. I'll have to figure out how to increase RH, without discoloring the fruits.

Cased another King Oyster block, and instead of leaving the top of the bag open, I may move into a mono tub with the bag clipped slightly closed (really not sure on best practice here).

 

Taped up every hole on my random-holed shotgun fruiting chamber. May move some of these next blocks in there as they fruit, and treat it as a huge monotub with the bags closed in the box. Not sure if perlite should be used on the bottom.

Any help would be appreciated on designing a fruiting chamber or steps others have had to take in a terribly arid climate.

 


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#27 Auhron

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Posted 18 January 2021 - 10:12 PM

Super pumped! After two years of trying to print Lions Mane, it finally happened.

It should not have been this difficult!

 

A very mature specimen was stuck in a SAB with armholes covered, tinfoil was literally wrapped around the fruit. A bowl of water was left at bottom of SAB for 3 days and no spores were visible on foil.
Yesterday the SAB was misted with H2O, and I guess getting the humidity up triggered sporulation! The foil was covered in dense dense white spores. I am currently printing again hoping to get a few more prints.

Now on to trying to swab or print some King Oysters.

How many days old was the Lion's Mane that you managed to print? I'm going to try to get some spores off one in my next batch of fruiting blocks.  I might try the same method you used, or skewering one and suspending it over a sterile jar and let the spores drop into the jar.

 

Also https://mycotopia.ne...-2#entry1470949 that's my fruiting chamber. I just have to fill the humidifier once a week(ish). The Inkbird humidity controller attached to the ultrasonic humidifier is phenomenal as winter here is brutal for relative humidity, my house sits at around 30% rh. I watched a couple Youtube videos about making an automated monotub, that was the result of my efforts. 

 

Flipping the bag over to fruit from the bottom is what a few commercial cultivators do for their second flushes, ex: WTFungus - they have a youtube video on just that for Lion'sMane (which apparently won't fruit from the same surface twice), and their King Oysters. I am attempting this for my second flush from my previous LM block.



#28 rockyfungus

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Posted 18 January 2021 - 10:32 PM

The fruit is still on the block and heavily browning. I'd say it's 3-4 days past where I normally pluck em. Which I believe is about their shelf life.

 

I'll have to check out their lion manes and oyster videos.

Been meaning to automate forever and this will push me in that direction as I guess I've lucked out on seasonal humidity.



#29 rockyfungus

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Posted 23 January 2021 - 02:03 PM

2 blocks of my original is fruiting (original in fruit, weird new genetics in back)

LM.JPG

and this is the new stuff...not sure what the hell this is

Wtfmane.JPG

 

trying to automate an old grow tent. Swamp cooler and a simple fan are being tested currently. Hygrometer reading 90% and looking like it's not liking that much humidity!

 

got some enoki and kings eating some masters mix. Shiitake making quick work of grain (Super thin compared to any myc I've seen)



#30 Auhron

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Posted 23 January 2021 - 04:31 PM

Ooohh, Doesn't quite look like Lions Mane...  Perhaps it will fill in as it grows.  Lots of pictures please, I'm curious to see how it turns out.  Did you do anything different with that particular culture at any point?



#31 rockyfungus

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Posted 23 January 2021 - 07:17 PM

Nah, looked like any other block/agar that's why I fruited them in same place. Just genetics that were "wild forged" from Oregon.

My "reishi" looking jar is lions mane too, unless I mixed those up some how.


Edited by rockyfungus, 23 January 2021 - 07:18 PM.

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#32 rockyfungus

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Posted Today, 07:35 AM

Took some time off work, feels strange...Been working on automating an old grow tent. Nothing fancy just a swamp cooler blowing into the tent, and a small ultrasonic humidifier on floor. Need to figure out some timing cycles with my time off.

Getting a bit frustrated as I've never seen my fruits discolor, or produce exudates this badly. (too much humidity?)

LM1.JPG

LM2.JPG

WeirdlM.JPG

that's the weird one, filling out



#33 Auhron

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Posted Today, 08:04 AM

Funky looking, I have read that Lion's Mane is sensitive to direct misting, and will bruise as a result. Optimal RH for fruiting between 90-95% according to Stamets. Humidity above 95% during fruiting may also lead to discoloration. Other than that I can't really offer any informed input.

 

I hope you find the solution.



#34 rockyfungus

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Posted Today, 01:23 PM

My Hygrometer was reading 81% and my inkbird roughly 70%. I'm afraid to up the humidity to the 90s as it's already looking too moist.

Not sure if I should dial the tent in with something easier like cubes, then move on to gourmet.
 

Started with a swamp cooler in the tent, but it only got humidity up to 50%. Then moved swamp cooler out and opened a side flap to "exchange" the air. Have a small oscillating fan inside and ultrasonic on floor. "Blurple" light for a bit of UV/IR hopefully. (minimal research put into this)

Tentversion1.0.JPG



#35 Auhron

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Posted Today, 02:41 PM

Hopefully someone with more experience than myself can weigh in with some ideas to help you out.






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