I've just had bad luck with standing water on my plates.
So if the syringe left a puddle usually slime mold or bacteria takes over. I've had better luck with a loop to truly get less then a drop on the agar. Sounds like you just got bad syringes though
First Agar Attempt
Posted 30 December 2020 - 11:20 PM
I've just had bad luck with standing water on my plates.
- Arathu likes this
Posted 01 January 2021 - 06:23 PM
So I took a couple transfers from the most advanced GT cultures. I tried to get the most rhizo looking stuff, but I'll probably need a few more transfers to get everything clean and isolated. I also took some gills from a B+ (IIRC) isolate I had laying around and put them to agar.
Will post pics once there is something to see.
- Mycol likes this
Posted 06 January 2021 - 09:03 PM
Here are the "good" transfers from the other day. They pretty much all had some bacterial hitchhikers but I see a few good spots for transfers.
On this first one, the myc has been having a difficult time getting past the bacteria on the transfer. But the section I marked is where it made it to the fresh agar and will grow out far enough to transfer from.
This one is similar to the first, but the myc is having more trouble. I'm mostly keeping it to see how it fares. Hope is not high.
The myc on this transfer seems pretty aggressive and I'm excited to see how it does on the next cleaner transfer.
This is the cleanest of all the transfers. It's got a nice mat going with plenty of great looking sectors.
And lastly, this is the jar I threw a few pieces of gill from a B+ isolate from a FOAF. Within 3 days there was visible myc, and these things have been in a jar w/ silica gel for 6mo!
Edited by HrVanker, 06 January 2021 - 09:21 PM.
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Posted 07 January 2021 - 11:47 AM
Always super difficult to tell from pictures. Your first 2 jars I'd toss though, both I can't discern any healthy myc.
When you look at pic 3 it has healthy obvious myc, but right at your transfer is that same slime/bacterial presentation.
Great pictures, just so hard to discern without seeing from top/bottom/side.
- HrVanker likes this
Posted 07 January 2021 - 12:57 PM
I definitely don't have high hopes for the second jar.
The third jar has a bunch of slime on the left side, and a bit on the upper left outer edge. But everything to the right is clear and free.
The downside to jars is the lack of good photos, other than that I do like them. I may try pouring some proper plates, but not right now.
Posted 08 January 2021 - 07:20 PM
Yesterday I made a few more transfers. So far all but one are showing signs of recovery, puffing up and making visible progress towards the new agar! I have a few different agar recipes floating around: chunky WBS agar, LME (dark blue), and a new 50/50 WBS/LME (light teal/green). So for each of the transfers from yesterday I tried to make sure that each one was on a new type agar, just to see how they do.
I made T1s from 4/6 of my Redspore germ plates:
Each of these are T2s from my GT plates:
I made 6 of these, but these are the only two worth showing at the moment.
And lastly, 3 T1s from the germinated B+ gills:
I also made up 10 4oz jars of WBS to test some of these cultures (I plan to make mini tubs with some or 10oz containers). And since the PC was running, I made 3 plates of plain distilled water agar. Planning on testing them out with some bacterial transfers to see how it goes.
- adrian118 likes this
Posted 21 January 2021 - 06:09 PM
Last week I made some transfers from some of the jars in my last post. Most of them are doing okay, nothing to write home about. But I will say that the culture from the B+ gill is looking really good.
I made two transfers from a T1 B+ jar (WBS/LME). One went to my last chunky WBS-agar and the other went to a WBS/LME agar. The chunky WBS specimen is looking remarkably rhizomorphic:
The WBS/LME is looking good, but not nearly as thick:
Each of the cultures I transferred from last week also went to grain. I used five 4oz grain jars and picked the best remaining sections. So far, 4 are showing some nice looking growth. The grain does look a bit dry, so I am slightly concerned about that. I may end up adding some distilled water if things start to slow down.
Being concerned about the grain jars moisture, and having a few plates that were being overgrown I decided to do the rest of the jars on Tuesday night. One plate had a bunch of knots and a tiny pins, most were so-so looking. There was one particularly stand-out plate that was a GT culture that grew out in thick straight rays, so I'm curious to see what happens.
My plan is to fruit in 8oz containers inside a few sterilites. Then take a couple of clones and prints from each jar and continue working with the genetics.
Edited by HrVanker, 21 January 2021 - 06:34 PM.
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Posted 21 February 2021 - 01:50 AM
Okay, so here are a few of my test jars spawned to coir:
Since I started this thread, I have been saying that I had GT spores going. However I see that I still have my GT syringe in the fridge, so by process of elimination the strain is EQ.
The container closest to the camera is fruiting slowly, showing a couple blobs in the upper left corner, and some very PE fatboy looking traits. It will be interesting to see how they develop. This culture had been transferred a couple times.
The middle container was an MS plate that started pinning, so I threw the entire thing into a test jar. All 3 containers were spawned at the same time, and this one had full on pins when I put them in the sterilite. I've already pulled and printed 6 caps, and took clone from the first mature fruit.
The far container is also fruiting a little slower, so far nothing too remarkable.
I have some Rustspore and more EQ test jars spawned, but two of the three RS seem to be going bacterial. I lost my one B+ test jar because it went bacterial after spawning as well. I am chipping bits of coir off of a 10lb block, and I'm having a lil bit of trouble getting the water content right, I suspect this to be the primary reason for the bacterial losses. All the jars were fully colonized, most of them starting to knot up a bit.
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Posted 29 March 2021 - 05:19 PM
So I figured I'd start updating my own thread rather than continue hijacking the mycogone thread that I posted in previously.
I harvested a couple of the Redspore/Rustspore "eyeball" fruits last night, and they seem to be pretty dense. Today there were one or two more ready for harvest, and it looks as though one of them started dropping a small amount of spores! That cap is currently on foil, while the body is in the dehydrator. It would be really cool to isolate this mutation and see if I can do anything fun with it. I have some EQ clones and prints which had very nipple-y caps, and I'm thinking it might be fun to try and stabilize both the eyeballs and nipples, and see if I can cross them to get nipple-y eyeballs! But that's getting way ahead of myself.
As I mentioned in the mycogone thread, these fruits are the 3rd or 4th xfer from a germ plate. Am I correct in thinking that if I were to clone any of these fruits that they would still be 4th/5th xfers and near senescence?
Edited by HrVanker, 29 March 2021 - 05:26 PM.
Posted 30 March 2021 - 05:37 AM
I'm going to keep watching and taking mental notes as I have just purchased my supplies to try my own first agar adventure.
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Posted 31 March 2021 - 03:20 AM
We'll see if I ever get to that point. It's going to be lots of agar, flushes, and prints before I get close. Lol
I also REALLY wanna see nipply eyeballs.
It appears that these guys are dropping spores. The first fruit had a very lite print, the second is a bit better.
I double checked the order that all these spores came from, and there were no KSSS in the order. And I'm like 95% sure that these are from prints I made from normal looking cubes.