11 replies to this topic

[groo]

So A little background I was watching myc tysons vidoe and he talked about this pyrex glass media bottle with a special pouring lip edge breaker.

 

Then I found the 2l bottles that were auo clave safe but they were $900 for 3 they have a built in edge lip so agar pours like a dream.

 

https://www.usplasti...px?itemid=31957

 

Conduct trace metal analysis, high and low-temperature work, or rigorous cleaning with these bottles. Bottles feature a certification to contain less than 20 particles per mL at 0.3 and greater. Metals certified to micrograms per liter (ppb) levels. Each bottle is double bagged under Class 10 laminar flow hoods inside a Class 100 cleanroom. The product includes a certificate of quality that assures the product has been tested and accepted.

• Teflon^® FEP bottle
• Tefzel^® ETFE cap
• Leakproof
• Excellent for storing high purity chemicals
• Autoclavable
• Temperature range: -105°C to 150°C
• Meets FDA standards
• Cap Size: 38/430; Neck ID: 24mm
• Dimensions: 121mm Dia. x 246mm Hgt.

 

THEN i found them on ebay for #30 each and 15 shipping or similar and then I offered the guy $90 and i was Im gonna be slinging agar but these are way too nice for what i need will you help ouy a mycologis and sell me 4 for 90 and he did so for the volume

 

https://www.ebay.com...872.m2749.l2649

 

I am not associated with any of the people and i actually did get 4 of the bottles and a 1 l bottle from tysons video that was is amazing0 and thats a hell of a price.

I have not yet poured agar with the 2l bottles yet

 

 

 

this is a link for an ebay search "nalgene fep" https://www.ebay.com...ne fep&_sacat=0

 

 

All that stuff has some amazing properties such as remaining flexible under the refrigeration temp of liquid nitrogen, Able to withstand infedinite PC cycles.

Edited by groo, 26 December 2020 - 01:42 AM.

[Saphroziac]

Seems like a mistake...what a wild price.

[groo]

https://www.thermofi...0001#/1600-0001 nope keep in mind 2l bottles are huge and

the 35ml are $55 each.

 

2 pieces directly from thermofisher is $820 for 2 keep in mind there are the 2l bottles.2l is huge. That is soooooooooooooooo expensive.

Edited by groo, 26 December 2020 - 03:39 AM.

[jrh]

I think those bottles are too big. Here is what I use...

 

https://www.amazon.c...0?ie=UTF8&psc=1

$16.99 w/ prime shipping

 

What size are the bottles you ordered? Will they fit in your PC?

 

 

[Moby]

I payed 30€ ~ 33$ shipping included for 1x 100ml bottle, 3 x 500ml bottles, 1x 2000ml bottle and 1x 1000ml bottle on ebay.

 

 

Third 500ml bottle is outside right now.

 

Brand "Simax" - a Czech producer (europe).

 

 

Says heat resist up to 140C

 

The price on those other bottles is a joke man, honestly.

Simax sells 20000ml autoclave bottles for, I think it was 180€

 

Don't want to destroy your excitement dude, the price is just ridiculous

[newmoon]

Proper lab equipment is outrageously expensive because:

1. the cost is generally put on research grants (where $100k for a project is a small amount), and so no one cares too much about price

2. liability is very high for the manufacturer - equipment failure can result in the loss of weeks or months of expensive work

[TVCasualty]

I use 1L glass mineral water bottles for making agar. They are autoclavable, pour well, and the price is hard to beat (I get mine from my recycling bin).

[Myc]

Who actually "pours" agar?

 

I use disposable 60cc syringes as featured in this article:

https://mycotopia.ne...s-banzai-style/

Scroll down to post #9 to see the syringe at work.

Edited by Myc, 26 December 2020 - 11:57 AM.

[TVCasualty]

Who actually "pours" agar?

 

I use disposable 60cc syringes as featured in this article:

https://mycotopia.ne...s-banzai-style/

Scroll down to post #9 to see the syringe at work.

 

I used to.

 

But then I saw that TEK you posted that I'd somehow overlooked back when he first posted it and now I'm thinking I might not pour it anymore, either.

[groo]

Yes 2l is way too big. Even 1l id too big my arm hurts after pouring 1l

 

Imagine the 2l face palm what was i thinking anyway.

 

Its not feasible to pour 2 or 3 stacks of plates with a syringe although very cool. I am developing a cheap method of putting a tube directly out of a sterilizer and run it through a  pump. Or make agar and have tube inside with ends covered and tube sterilized with everything then pull out on front of flow hood.

 

https://www.ebay.com...u8AAOSwaOtfqedD under $100 then It will make a lot of sense to use the 2 l botles.

 

looks like serviceable used equipment.

 

With the volume myc tyson pours, (2000 plates per week) it would make sense for him to use one of these units and lift stack insert tube tap foot pull out tube replace lid pick up next, 

 

Like this but instead of automatic you lift and hot the foot button

[Direct Link]

Edited by groo, 26 December 2020 - 08:22 PM.

[Saphroziac]

You might be able to find some useful stuff from these guys. I order everything from them so far.

https://www.scbt.com...ers/_/N-1u991z5

Edited by Saphroziac, 26 December 2020 - 08:27 PM.

[Moby]

I see what you say why lab equipment is so expensive but man, theres no reason as a private person for cultivation purposes to pay a price that high if theres option that work perfect and cost 50+ times less (inacurate number but you get the point).

 

I don't pour, the 2l bottle is my drinking bottle lol, easy to clean - the healthiest way to consume water.

Also used for extractions, the 2l leaves good room for bigger projects and is great for ethanol- waterbath extractions (closed lid prevents alcohol loss) and definately safe for the used temps without any issues.

 

1l bottle I use to measure 700ml 99.99% isoalcohol and 300ml water for the workspace sanitizing, the plastic thing on top allows spill free filling of spraying bottles and smaller bottles.

 

Until a flowhood finds its way into my lab space, I'll keep sterilizing filled plates and do batches of 6-12 plates at a time (different size plates), non commercial of course lol.

 

Just my piece of cake.

 

A fully automated plate filler sounds badass, well this will be the way to go for commercial growers in the future