I couldn't make it through the whole thread but guess I got the most important things said here.
While I disagree with FunG on most things I read around the platform, we had some exhausting conversations, the general idea to dilute a spore syringe IMO is ok for people who do only work with spores and no agar or LC and do alot of inocs in for example pf jars.
Its a way to bypass agar work.
Clones then can be cloned directly to fresh pf jars.
Not that I do either method of those two but its possible.
To go back to the original question "Expand mycelium? A lot for a little"
I would straight answer that with biopsy to LC, its the to my knowledge best method to expand a tiny sample of mycelium into a jar of liquid culture that can inoculate a ton of grain.
Some guys here have explained how they would start from spore.
Keep in mind that your transfer plates multiply exponetially with every transfer you do.
I personally do only one spore plate and transfer that depending on my goal - just to produce some prints, one transfer and then inoc jars or - until the culture shows agressive growth and separate that to a single final plate which then inocs multiple jars that can be fruited in small trays.
By splitting the final plate into lets say 6 trays that are fruited separately, you still have different substrains fruiting and with the transfers made sure they all are agressive colonizers.
IMO to make 3+ plates with the same variety and do transfers on all of them is ALOT of work and time invested into a single variety, wasted energy IMHO.
By then cloning from the first flush you already got the second part that adds up to the "agressive colonizer" and thats "fast fruiting".
If you then clone a good cluster on the next generation you will increase your canopy and "add" (in reality you don't add anything, you just sort out all of the not so good strains) another good property to your culture.
Theres for everything different ways to go and I personally prefer going this route instead of doing 20+ plates of a single variety with all the transfers and clone plates and 5+ plates that in the end need to be fruited to test them out.
I select some good growth from a single plate and spread that to a final inoculation plate that then will be used to inoculate jars with the different sectors.
Edited by Moby, 13 February 2021 - 03:34 PM.