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Sawdust in your agar?


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14 replies to this topic

#1 timothywyllie

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Posted 21 January 2021 - 08:35 PM

Anyone try it? Even worth it? As long as the quantity is low, visibility may not be an issue.

#2 Arathu

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Posted 21 January 2021 - 08:43 PM

I wouldn't even hesitate, wood tea's too, dry them cracker dry and powder them with a mortar and pestle.......

 

Add like any other additive to an agar mix.........I do

 

A


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#3 hyphaenation

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Posted 21 January 2021 - 08:44 PM

You can.. for sure, put soaked sawdust in agar. You can also make sawdust soak-water and use that as the water in your agar. Alder is really good for using soak-water.


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#4 rockyfungus

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Posted 22 January 2021 - 12:01 PM

Anyone use oak dowels in slants? My goal is to be able to have a slant culture as well as the dowel for inoculation.

#5 Arathu

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Posted 24 January 2021 - 09:00 AM

Anyone use oak dowels in slants? My goal is to be able to have a slant culture as well as the dowel for inoculation.

I don't use them in slants but definitely for making plugs. In fact I've put sterilized plugs right on top of colonized agar, it's very slow which is probably good for long term storage. Needles has technique for storing cultures in sterile water. Many ways to skin the cat (not our cat) so to speak......

 

A



#6 Myc

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Posted 24 January 2021 - 12:01 PM

Here's a fun article to check out:

https://mycotopia.ne...s/#entry1153405


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#7 rockyfungus

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Posted 24 January 2021 - 01:08 PM

Whenever I think I made something, someone has a way more elegant & cost effective way!


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#8 Myc

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Posted 24 January 2021 - 01:53 PM

I was actually looking for a follow-up article when I found that one from 2014.

Unless I'm mistaken, someone else made a thread using popsicle sticks or hobby sticks used for spreading glue and such.

Hyphaenation has a ton of fascinating articles in his catalog. You should give his work a browse.



#9 Arathu

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Posted 24 January 2021 - 02:05 PM

Whenever I think I made something, someone has a way more elegant & cost effective way!

AND....In that manner, using a mechanism such as these forums,.......WE grow exponentially.......

 

IMHO

 

This is the New Library at Alexandria......

 

A


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#10 Arathu

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Posted 24 January 2021 - 02:16 PM

Here's a fun article to check out:

https://mycotopia.ne...s/#entry1153405

Old colonized woodlover chips from a long neglected bed will in many cases fire right up when presented new wood food or placed on wet cardboard......

 

The coffee stirrer in the slants is brilliant......thanks for pointing to that Myc.....

 

I love it.....need to order some screw cap test tubes methinks....

 


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#11 rockyfungus

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Posted 24 January 2021 - 04:32 PM

Decided to check my oak dowel slant, looks like king stroph took to it.

 

WIneCap.JPG


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#12 cujoloki

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Posted 25 January 2021 - 08:40 AM

Looks colonized! These slants are on my list of supplies i want. That and a stir plate! Good work on those slants Rockyfungus!



#13 rockyfungus

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Posted 30 January 2021 - 09:35 AM

Used a loop to move some "myc" from the slant to 2 plates. Both turned green within a day, I have such horrible success with loops...



#14 cujoloki

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Posted 30 January 2021 - 06:04 PM

Did you sterilize the loop first?

#15 rockyfungus

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Posted 30 January 2021 - 08:07 PM

Did you sterilize the loop first?

I sanitize with alcohol the whole handle/loop. Then I flame the whole loop/metal arm. Not sure if the handle can be PC'd though.






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