69 replies to this topic

[newmoon]

I believe they're saying that this is a new species in section zapotecorum; so, it's not Psilocybe zapotecorum but doesn't yet have a name. Sections are messy taxonomic categories between genus (Psilocybe) and species.

[smellitstinknot]

JR, the pic is Australian Zap.

Thank you for the great info and for distributing genetics Stinknot! To clarify, you are saying the Australian zapotecorum is zapotecorum and Auckladiae is a separate species or that they are the same?

 

Yes the Australian species is a separate species new to science. Psilocybe Aucklandiae is a temperate species occurring in Auckland, New Zealand. The species closely resembles Psilocybe zapotecorum but there are microscopic differences. Sect zapotecorum is only known to occur along the east coast of subtropical/tropical Australia. I've uploaded photo's of this species on mushroom observer for anyone interested. https://mushroomobserver.org/361911 and pictures of cultivated specimens.https://mushroomobserver.org/401380

Edited by smellitstinknot, 29 January 2021 - 10:42 PM.

[Celestialexplorer1]

Well I am determined to find a zapo strain that is faster than what I've seen this far so what I'm doing with several species is isolating all the growth I can no matter what it looks like and then singling out stuff to working with from there. Keeping the spore plates too to work on as it progresses. Also doing this with neoxalapensis and papuana

[Jrotten]

Ok lets get some pictures up. Here is Aussie on sub spwaned three days ago and Zapotecorum var Huatala (sp?) from Mexico on agar received as a culture from a member here. None of my Aussie plates looked like this or moved this fast on agar but it was an experimental coffee agar and nothing looked right on it. I've gone back to standard MEA for everything including the Mexican Zapo.

Hypothesis: wetter is better.
My Semperviva have always preferred a little wet to a little dry so long as they didn't contaminate, which the extra water did cause more of. I see a similar tendency so far in the Aussie I think.

Supporting Observation: on slightly dry grain the Aussie did better at 70F in a drawer than 75F in an incubator. All other species including Semperviva moved much faster on the same grain run. Now on substrate, back in the incubator at 75F the wetter sub seems to be grabbing the sub faster. Both trays have recovered faster in the wetter environment of the tray with no gas exchange even in the incubator. 3 days to completely fuzz and start to expand is actually pretty fast for these jars I have thought were dead many times. I have small hope which is brighter seeing an improvement in performance so far on sub.

As far as the sub goes, it is the result of lots of uncertainty. It is one part tumbler compost (old cow manure, straw, coffee, lawn clippings, failed subs of cubes, semperviva, pans, with lots of slug activity to chomp away at fungi), fine oak saw dust from floor sanding (virgin oak floors, no chemicals), 1/2 part spent coffee ground, 1/2 part wheat bran... that whole combined volume coir, and then a very large part of sand and finally a large part of straw. The first part was an experiment that just felt too rich so I cut it with straw having read that zapotecorum likes sand and straw somewhere. The compost+coir 1:1 is my standard substrate for everything except pans.

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Edited by Jrotten, 30 January 2021 - 04:19 PM.

[Celestialexplorer1]

aussie zap on masters mix. Just a little experiment. Other tub is almost done. Still unsure of fruiting methods. My plan for this little container was gonna be just to case it and fruit it in the cup. Just as a small attempt to see what conditions it likes and maybe get a fresh print or two.

[Jrotten]

The Aussies on sub continue to fuzz out and are much brighter white and fluffier than when I’m grain... but it’s hard to tell if they are really grabbing the sub yet. I always get nervous when things continue to fluff up for days after spawning. I am continuing to run them pretty cool though. Upper 60’s to low 70’s. My incubator is full of grain jars and my FC needs some maintenance to the point I might have to toss some things to clean it up and make room for a cube grow I’ve put off too long, but I would really like to get it in a warmer environment. The Mexican Zapo proper doesn’t seem to mind.

I can’t judge speed anymore after concentrating on Semperviva for 6 months and then kicking pans into high gear. If it doesn’t colonize in 5 days and pin in 5 more it feels painful right now. I have species whiplash. Whiproom? Mushlash? IDK

[ChocolateStarfish]

I am almost caught up to you now!! so excited to be so close to having fruits of all of these new species! Im incubating 2 isolates of the ausi zapo in LCs and in a day or 2 im going to pump 60 ml of each LC into 2 different 5 lb myco bags made with grains, vermiculite, some manure, some coir, some sand, and some worm castings. I plan on casing it witha pasteurized coir/verm/rye grass seed mix, as I try to get grass to grow in the casing as I feel it helps balance out the environment since the mushrooms are putting out CO2 and the grass breathe that in and breathe out fresh oxygen, it makes it to where you need less gas exchange, but still get primo mushroom growth... in my opinion of course

Edited by ChocolateStarfish, 03 February 2021 - 02:12 AM.

[Celestialexplorer1]

The Aucklandii is moving so much faster on grain than zapo did. It's close to cube speed 2lb but of spawn in 14 days. My small zapo tub is almost finished after 3 months but it looks like there is two types of myc in it which worries me. The original type was grayish and and the faster is rhizo like and bright white you can see the difference here.
I don't know if since it's been colonizing for three months it's some type of overlay or if it's something that might have creeping in through the filter. Honestly don't know what to think here. There's still a corner I'm waiting on to colonize before I'll think about trying anything with it

[bezevo]

So i see  at least one of these is from a temprate zone ?  Are they both from temprate climates ? and how cold are these winters compared to  US climate zones ?

you are saying the Australian zapotecorum is zapotecorum and Auckladiae is a separate species or that they are very close . i am always looking for genetics that might naturilze in u.s. ag zone 6 to zone 5 ? dose anyone here working with these know ?

 

 

smellitstinknot :

Psilocybe Aucklandiae is a temperate species occurring in Auckland, New Zealand. The species closely resembles Psilocybe zapotecorum but there are microscopic differences.

[coAsTal]

Here's their native climate-- seems very compatible with many areas in the US:

[Jrotten]

Auklandiae is temperate. The unnamed Aussie Zapo, if it corresponds to Semperviva would be tropical to subtropical, growing at the higher altitudes of Mexico etc.

So an experiment I did because I had such sparse germination was to take my three favorite cultures, put them on grain with a 1 wedge per jar, and also did one jar with a wedge from all 3 of those cultures. At first I thought the combo jar just did better because it was a 3x inoculation, but on sub now, next to the next strongest single, it seems to be grabbing the sub much better as opposed to fuzzing out into the air. The other two are just now looking worthy to put to sub, so I’ll know more, but I may have a sleeper culture from grain, OR there may be a benefit to a more diverse genetic combination.

[smellitstinknot]

Yes that's right. The area I find sect.zapotecorum is subtropical and has a 365 day a year actives season. In summer you can find pans,papuana,zapotecorum,cubensis and another new dung loving species recently discovered. In winter you've got P.semilanceata,alutacea,gyms,subaeruginosa,tasmaniana and probably P.aucklandiae (unofficial reports) . I have actually found sect.zapotecorum growing next to P.subaeruginosa in autumn. Also it's worth mentioning P.papuana and sect.zap grow at high altitude in areas where the winter temps get down to -10c so a freeze doesn't phase them.

[YoshiTrainer]

Great info Stinknot, thank you for sharing! What a magical place to have actives growing 365!

[ChocolateStarfish]

Yes that's right. The area I find sect.zapotecorum is subtropical and has a 365 day a year actives season. In summer you can find pans,papuana,zapotecorum,cubensis and another new dung loving species recently discovered. In winter you've got P.semilanceata,alutacea,gyms,subaeruginosa,tasmaniana and probably P.aucklandiae (unofficial reports) . I have actually found sect.zapotecorum growing next to P.subaeruginosa in autumn. Also it's worth mentioning P.papuana and sect.zap grow at high altitude in areas where the winter temps get down to -10c so a freeze doesn't phase them.

 

 

 

Do you live in new zealand?? 

[YoshiTrainer]

Ps. Zapotecorum "Chico Nindo", waking up in it's new home.

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[Celestialexplorer1]

I just put some zapotecorum "Jalisco" to agar last night. The Aussie zapo will go in fruiting really soon.

[coAsTal]

I have also just put new Zap spores to agar-- here's hoping this time I can get some germination!!!

[Jrotten]

My spawn doesn’t seem to be running with the vigor of the wedges. Probably going to expand and do more jars, and then because Of what I did I might use a syringe filter to add some hydration to the jars. Nothing to lose it’s an easy enough experiment. The saw dust makes it impossible for me to gage anything. Way over did that except my Gym Purp is going crazy on it and that’s exciting.

[coAsTal]

Just a bump to tell everyone I have finally gotten germination on one of my new Zap "Jalisco" plates-- it's going to require cleaning up I can already tell from the splotchy start to the nock points, but we're alive!

[Jrotten]

Alright I’m going all “IDFK” with Zapos and Caerulescens and spawning about 2 cups of WBS+sawdust to my Semperviva substrate of 45/45/10 coir/compost/vermiculite except I added a big dose of oak sawdust, spent coffee grounds, and then to that about an equal volume of chopped straw.

I’m pasteurizing in bags and will probably just fruit from them. Same as my first Semperviva test grows as well as the one I’m currently test driving.