Paradox
©
Fisana

Jump to content


Photo
- - - - -

An AA+ Agar Adventure


  • Please log in to reply
18 replies to this topic

#1 pastyoureyes

pastyoureyes

    Manure Muscler

  • OG VIP
  • 869 posts

Donator


Awards Bar:

Posted 31 January 2021 - 12:23 PM

I started from one of the two LCs below which were both made using a spore print.
gallery_150062_1520_949416.jpg

One drop of LC was placed on the plate below and allowed to grow out. There were no signs of contamination. Four wedges were cut indicated by the arrows.

gallery_150062_1520_1344318.jpg

Each wedge was transferred to a new plate and allowed to grow out.

gallery_150062_1520_1096927.jpg

A syringe was poked through the lid and the needle used to scrape up mycelium from the outer edge. Sterile water from the syringe was used to suck up the mycelium. The area circled on each plate is where mycelium was taken from.

gallery_150062_1520_157357.jpg
  • sandman, Microbe, bezevo and 1 other like this

#2 pastyoureyes

pastyoureyes

    Manure Muscler

  • OG VIP
  • 869 posts

Donator


Awards Bar:

Posted 31 January 2021 - 12:30 PM

Four syringes containing mycelium from each of the above plates.

gallery_150062_1520_203160.jpg
  • makinbones69 and Choices like this

#3 YoshiTrainer

YoshiTrainer

    Onion tied to belt

  • Gold VIP
  • 820 posts

Awards Bar:

Posted 31 January 2021 - 03:37 PM

Cliff hanger! What is your next move, to grain, direct to sub......inject yourself?! :)

Are those baby bottles with the nipples inverted for your LC? Genius!
  • pastyoureyes likes this

#4 pastyoureyes

pastyoureyes

    Manure Muscler

  • OG VIP
  • 869 posts

Donator


Awards Bar:

Posted 31 January 2021 - 06:24 PM

Each syringe was used to create it's own LC.

gallery_150062_1520_60744.jpg

Cliff hanger! What is your next move, to grain, direct to sub......inject yourself?! :)
Are those baby bottles with the nipples inverted for your LC? Genius!

The baby bottle method is a very old school tek.
  • Microbe, FunG and YoshiTrainer like this

#5 pastyoureyes

pastyoureyes

    Manure Muscler

  • OG VIP
  • 869 posts

Donator


Awards Bar:

Posted 06 February 2021 - 12:05 PM

LC isolate #1

gallery_150062_1520_9660743.gif

LC isolate #2

gallery_150062_1520_7741184.gif

LC isolate #3

gallery_150062_1520_4312064.gif

LC isolate #4

gallery_150062_1520_11426286.gif
  • Riza, Microbe, bezevo and 3 others like this

#6 Moby

Moby

    Mycotopiate

  • Expired Member
  • 299 posts

Posted 15 February 2021 - 03:32 PM

Got a question.

I remember you prepared your bulk sous vide.

 

Just bought this guy to sterilize agar without having to babysit

36489002709-tmp5be04a5652b0d.jpg

 

it has a sous vide function, have you done sous vide casing pasteurization, if so how long and what temp?

____

 

Btw following the thread

 

Are you Pastywhyte from shroomery ?


Edited by Moby, 15 February 2021 - 03:34 PM.

  • pastyoureyes likes this

#7 pastyoureyes

pastyoureyes

    Manure Muscler

  • OG VIP
  • 869 posts

Donator


Awards Bar:

Posted 15 February 2021 - 06:46 PM

I have not used it to pasteurize casing, only substrate.
For substrate I've been using 150*F for at least 4 hours.
I would thing if you are pasteurizing a casing you could use the same time/temp.



That thing looks like it has a pretty small capacity for substrate.

I am not Pastywhyte.
  • FunG and Moby like this

#8 Moby

Moby

    Mycotopiate

  • Expired Member
  • 299 posts

Posted 15 February 2021 - 07:28 PM

I generally sterilize everything, bulk and casing included.

My bulk is filled into 870ml jars of which 14 fit into the presto 23qt at a time.

For spawn I use the same or slightly smaller jars 730ml and just bulk 1 jar spawn to one jar bulk, no pasteurized bags.

 

The electric cooker was originally only thought to sterilize pre poured plates for SAB work, not for bulk prep, I want to check pasteurized casing and see if theres any difference.

 

Tried to pasteurize some casing jars in the dehydrator but one casing grew green (spawn and bulk were clean, was in a transparent tray) so I don't want to try that again.

 

The pot has a total volume of 6 liters (6,34qt) but a liquid maximum of 4 liters, three jars 2x 870ml and one 730ml fit in, thats enough casing to case the 2x 19qt and 2x 33qt monos that I bulked today.

 

Gonna go for the 150f for 6h to be on the safe side, cheers!



#9 pastyoureyes

pastyoureyes

    Manure Muscler

  • OG VIP
  • 869 posts

Donator


Awards Bar:

Posted 16 February 2021 - 12:39 AM

I put about 6-8 quarts of substrate in a food saver bag. I can fit 2 of these bags in the sous vide/cooler combo with a little room to spare.

I use an electric PC on the slow cook setting to hydrate my grains. I can do about 6 quarts in mine.
  • Moby likes this

#10 Moby

Moby

    Mycotopiate

  • Expired Member
  • 299 posts

Posted 16 February 2021 - 08:28 AM

Thats cool, guess with bigger tubs I'd give that a try.

 

For spawn I use no soak, no boil millet, no pre cooking needed.

 

For stones tho I use rye that gets a long simmer, good idea to do the slow cook setting, gonna try that on my next stone run



#11 pastyoureyes

pastyoureyes

    Manure Muscler

  • OG VIP
  • 869 posts

Donator


Awards Bar:

Posted 17 February 2021 - 01:35 PM

Here's an update on the LCs

Isolate #1

gallery_150062_1520_4207688.gif

Isolate #2

gallery_150062_1520_15581187.gif

Isolate #3

gallery_150062_1520_11498754.gif

Isolate #4

gallery_150062_1520_18854997.gif
  • Sidestreet, YoshiTrainer, rockyfungus and 1 other like this

#12 rockyfungus

rockyfungus

    Mycotopiate

  • Free Member
  • 653 posts

Posted 17 February 2021 - 01:45 PM

Got a question.

I remember you prepared your bulk sous vide.

 

Just bought this guy to sterilize agar without having to babysit

Does that thing pressure cook?



#13 Moby

Moby

    Mycotopiate

  • Expired Member
  • 299 posts

Posted 17 February 2021 - 02:01 PM

 

Got a question.

I remember you prepared your bulk sous vide.

 

Just bought this guy to sterilize agar without having to babysit

Does that thing pressure cook?

 

 

 

Yes it does 1,8 bar = 26,1 PSI on a temperature of 241 fahrenheit.

Agar I do 30mins because of the lower temp, comes out clean.



#14 rockyfungus

rockyfungus

    Mycotopiate

  • Free Member
  • 653 posts

Posted 17 February 2021 - 02:11 PM

Nice, I use my electric one for Agar and small glass jars (baby food). Then g2g those glass jars out.


  • pastyoureyes, jrh and Moby like this

#15 pastyoureyes

pastyoureyes

    Manure Muscler

  • OG VIP
  • 869 posts

Donator


Awards Bar:

Posted 17 February 2021 - 02:32 PM

Nice, I use my electric one for Agar and small glass jars (baby food). Then g2g those glass jars out.


I used mine for agar once but now I PC much bigger bottles of agar to pour.
  • rockyfungus likes this

#16 rockyfungus

rockyfungus

    Mycotopiate

  • Free Member
  • 653 posts

Posted 17 February 2021 - 02:59 PM

I used the electric one for hot sauce bottles of agar, haven't done that in years. The electric one is great for syringes, reusable plates, scalpels etc. Typically I prefer a full sized PC for a media bottle of agar.

I just did a load of LC in small small unmodified round PP containers, worked really well. Didn't break the chunk up though.

 



#17 pastyoureyes

pastyoureyes

    Manure Muscler

  • OG VIP
  • 869 posts

Donator


Awards Bar:

Posted 17 February 2021 - 03:40 PM

I used the electric one for hot sauce bottles of agar, haven't done that in years. The electric one is great for syringes, reusable plates, scalpels etc. Typically I prefer a full sized PC for a media bottle of agar.
I just did a load of LC in small small unmodified round PP containers, worked really well. Didn't break the chunk up though.


You said PP containers.
  • rockyfungus likes this

#18 rockyfungus

rockyfungus

    Mycotopiate

  • Free Member
  • 653 posts

Posted 17 February 2021 - 05:12 PM

full of PP juice



#19 sandman

sandman

    That Motherfucker

  • Honorary Former Staff
  • 3,987 posts

Awards Bar:

Posted 17 February 2021 - 05:49 PM

Sad sanndman all out of likes :(


  • pastyoureyes likes this




Like Mycotopia? Become a member today!