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An AA+ Agar Adventure


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#21 YoshiTrainer

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Posted 08 June 2021 - 02:01 PM

Man, those are looking great!!!

Have you noticed any difference between isolates so far?
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#22 pastyoureyes

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Posted 08 June 2021 - 02:25 PM

Man, those are looking great!!!
Have you noticed any difference between isolates so far?

I didnt pay too much attention during colonization of the grain since I dont really care about colonization speed. All the tubs look slightly different but nothing drastic that you wouldnt see with multispore grows so far.
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#23 MushLuvR

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Posted 12 June 2021 - 01:25 PM

AA+ is the fastest cube I have worked with.  Once you have a nice Pinset... pop the corks and let her breathe... it will only take two days to finish.  I mainly grow PE or PAN's these days, but AA+ is an awesome Cube.  Better than Average colonizer in my experience.  

 

 

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4/4/21

 

 

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4/5/21

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Later that Day...

 

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4/6/21 the rest of them.  They matured so fast

overnight... a little sporulation, but no big deal


Edited by MushLuvR, 12 June 2021 - 01:27 PM.

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#24 pastyoureyes

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Posted 15 June 2021 - 12:27 AM

Tub #2 has distinctly whiter mycelium than the rest.
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#25 pastyoureyes

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Posted 19 June 2021 - 03:21 PM

Tubs 2 and 4 not looking good.
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#26 Sidestreet

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Posted 22 June 2021 - 06:22 AM

Yechh, I'm not sure what's going on there but if it's mycelium it doesn't look happy.  Do you see any drops of liquid forming on the extra-white masses?


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#27 coorsmikey

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Posted 22 June 2021 - 07:29 AM

Yikes! Looks like those things are going to explode with green. I hope it's not what it looks like from here.


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#28 rockyfungus

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Posted 22 June 2021 - 07:38 AM

I second the green diagnosis, seen a ton of it recently...


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#29 MushLuvR

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Posted 22 June 2021 - 12:49 PM

That doesn't look healthy at all unfortunately... sorry about your loss... always hurts a bit losing a project.  AA+ has beautiful mycelium and should look something like below.  Get back at it, humbling hobby at times, but dust yourself off and get back on that saddle.  

 

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#30 pastyoureyes

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Posted 22 June 2021 - 11:17 PM

I've been through many rounds with aa+ so it's no big loss. At this point I'm unsure if its contamination or just overlay. I would expect if it was contaminated it would have turned color by now, going to toss it either way. Might need to re test those LCs. Tubs 1 and 3 are fully colonized and I'm waiting to see some pins. Should probably start misting and fanning.
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#31 pastyoureyes

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Posted 23 June 2021 - 06:14 AM

Umm...

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Both taken from tub 4.

I also wiped a cotton swab over the suspicious part and no green or anything. Not sure what to make of this. Still suspect contamination as many of you have already said but this seems odd to me. Every part of me wants to toss these tubs. What do you guys think?

Edited by pastyoureyes, 23 June 2021 - 06:19 AM.

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#32 rockyfungus

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Posted 23 June 2021 - 09:39 AM

My AA+ culture always overlays and I tend to case or scrape. I'm over AA+ as it gave me such a hard time and so few fruits. Almost look more Ape then AA+, does Ape blob like PE?

 

Maybe it's one of those bubble contams?



#33 pastyoureyes

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Posted 23 June 2021 - 02:08 PM

My AA+ culture always overlays and I tend to case or scrape. I'm over AA+ as it gave me such a hard time and so few fruits. Almost look more Ape then AA+, does Ape blob like PE?
 
Maybe it's one of those bubble contams?


I havent had the problems you describe. Quite an easy and prolific strain IME.
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#34 rockyfungus

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Posted 23 June 2021 - 05:40 PM

I mean the average tray of mine overlaid just like your photos but less bubbly. I've ran clones, multiple prints, and even tried crossing with more "prolific" fruiters.


Wonder if you scratched some of the bubbly area if you'd get more pins. Keep us updated that's wild if it is overlay. Almost like pans before they are cased

Edited by rockyfungus, 23 June 2021 - 05:40 PM.

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#35 pastyoureyes

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Posted 25 June 2021 - 10:53 AM

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#36 pastyoureyes

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Posted 26 June 2021 - 06:42 PM

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#37 pastyoureyes

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Posted 26 June 2021 - 06:47 PM

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#38 pastyoureyes

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Posted 26 June 2021 - 06:54 PM

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#39 Oldpunk

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Posted 26 June 2021 - 07:46 PM

Huh.....you just throw the whole little pin in? Seems easier than cutting out a chunk of stem with a blade.

I'm a bit jealous. I was so excited for my AA+ grow and about 1/3 grew pale-ish.. But they didn't grow nice and white like that.
Your end results look good.
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#40 pastyoureyes

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Posted 26 June 2021 - 08:12 PM

Sure it's easier but I have no idea what the mature fruit should look like doing it this way.
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