Drilling PF Cakes
Greetings. I have been working with GLC to inoculate BRF jars. The myc in the GLC is chunky and it can clog needles pretty easy. I don't have any large size needles on hand so I wanted to improvise a "GLC Dabber" that would shoot a quantity of liquid culture into a jar. It was made by drilling a hole in a lid and inserting a needle tip that had it's end cut off and it was guerilla glued on the top side.
It worked fine on grain jars. I simply sterilized the dabber on a dummy-jar and then swapped it out with the lid on the GLC jar (in my clean area). Then I slide back the lid on each grain jar and gravity and the flick of a wrist put out exactly 3 cc of liquid every shot (I measured by dabbing into a syringe). Grain jars are a go and it's easy to dab into.
As far as BRF cakes go I was working on some jars and I realized it would be difficult with equipment on hand to use syringes to inject the GLC. With the verm layer on top it's kind of tricky to use the dabber to just pour the liquid on the top where the verm is.
I started thinking about the idea of drilling a hole with a clean chop-stick on the side of the jar. I tried it out and it seemed to "drill" okay... Next thought was, well that's cool but colonization would probably happen faster with an even start down the middle of the cake.
So I drilled a hole in the middle and each time i carefully pull out the chop-stick so as to not block the freshly drilled hole with debris. Put on my lids and breathers. PC'd that as per usual and when cooled I took my clean area. Opened each lid and dabbed the appropriate hole. I gave two shots to each jar or 6 cc GLC solution.
24 hours later you can see very good growth in all the jars! The side-drilled ones did very well and there is tons of obvious growth. To check the middle-drilled hole one has to flip the jar and look underneath.
Some myc definitely made it to the bottom verm layer...
Some good live myc trickled down to the bottom verm layer and you can see it growing ...radiating out 360. I'm happy with the way its going and its fun to try new things.
Keep you posted.
Edited by hyphaenation, 12 February 2021 - 02:38 AM.