Paradox
©
Fisana

Jump to content


Photo
* * * * - 5 votes

Microscopy With Microbe & Friends


  • Please log in to reply
47 replies to this topic

#1 Microbe

Microbe

    civis scientiam

  • OG VIP
  • 4,637 posts

Donator


Awards Bar:

Posted 26 February 2021 - 08:01 PM

Im starting a Microscopy thread and chose to post it here because it seems that this is still the most frequented sub-forum. If it is better suited elsewhere, the mods wills move it. Anyway encourage others to join in. Microscopy is amazing and although you can look at photos of specimens/subjects  'under the scope',  it truly does not have the same impact as seeing it with your own eyes.

Looking  through a microscope with our own eyes, brings you into the world of fungi, and other microscopic organisms, allowing you to see view its world from its point of view.  Everywhere in our daily lives, every solid surface, fabric, moving currents of air, our own skin, and etc. is crawling with microorganisms and its truly spectacular and fascinating  to meet them!

I would like to finish the introduction, with some very inspiring text.

*****Henry Baker, Chapter 15,"Cautions in viewing Objects" of The Microscope Made Easy, 1742 [A popular book in its time]:

"Beware of determining and declaring your opinion suddenly on any object; for imagination often gets the start of judgment, and makes people believe they see things, which better observations will convince them could not possibly be seen; therefore assert nothing till after repeated experiments and examinations in all lights and in all positions.

When you employ the microscope, shake off all prejudice, nor harbor any favorite opinions; for, if you do, 'tis not unlikely fancy will betray you into error, and make you see what you wish to see.

Remember that truth alone is the matter that you are in search after; and if you have been mistaken, let not vanity seduce you to persist in your mistake.

Pass no judgment upon things over-extended by force, or contracted by dryness, or in any manner out of their natural state, without making suitable allowances.

There is no advantage in examining any object with a greater magnifier than what shows the same distinctly..."


*****Robert Hooke, in Micrographia ,1665 [the first "microscope book"]:

"For the limits to which our thoughts are confind, are small in respect of the vast extent of Nature itself; some parts of it are too large to be comprehended and some too little to be perceived. And from thence it must follow, that not having a full sensation of the Object, we must be very lame and imperfect in our conceptions about it, and in all the propositions which we build upon it; hence we often take the shadow of things for the subftance, small appearances for good similitudes, similitudes for definitions; and even many of those which we think to be the most solid definitions, are rather expressions of our own misguided apprehensions then of the true nature of the things themselves. ....."

..."The texture of Cells of Cork and of some other frothy Bodies could not be so curious, but that possible, if I could use some further diligence, I might find it to be discernable with a Microscope. ... me thinks, it seems very probable, that Nature has in these passages, as well as in those of Animal bodies, very many appropriated Instruments and contrivances, whereby to bring her designs and end to pass, which not improbable, but that some diligent Observer, if helped by Microscopes, may in time detect. "

"...by the help of Microscopes, there is nothing so small, as to escape our inquiry; hence there is a new visable World discovered to the understanding

....By this the Earth it self, which lyes so near us, under our feet, shews quite a new thing to us, and in every little particle of its matter, we now behold almost as great a variety of Creatures, as we were able before to reckon up in the Whole Universe it self....



***** "The gifts of microscopes to our understanding of cells and organisms is so profound that one has to ask: What are the gifts of the microscopist? Here is my opinion. The gift of the great microscopist is the ability to Think with the eyes and see with the brain. Deep revelations into the nature of living things continue to travel on beams of light."

Daniel Mazia
  • sandman, fahtster, Myc and 5 others like this

#2 sandman

sandman

    Saint PP

  • Honorary Former Staff
  • 4,133 posts

Awards Bar:

Posted 26 February 2021 - 08:09 PM

Looking forward to your interesting threads developments! 


  • Microbe likes this

#3 Microbe

Microbe

    civis scientiam

  • OG VIP
  • 4,637 posts

Donator


Awards Bar:

Posted 26 February 2021 - 08:11 PM

So today i brought out my Metallurgical scope as it was the easiest accessible, and cleaned the dust off it.......man-alive there was a lot. I put a old plate under the objectives to see what i could capture, and although i was impressed, i was even more so when i ran my objective down too far and cracked my plate cover (very bad for objectives btw). Once cracked, i knew it was compromised,  so i just took the cover off and captured some really great low magnification photos.

I need to invest in a quality microscope camera. Im also limited to low magnifications until i get my illumination source and positioning corrected. I can't get light on my specimen using my higher objectives as im using external top lighting via LED A19 bulb in the 3700 spectrum.....horrible illumination source.

The plate was viewed under a 10x ocular and 5x .1 objective, and 10x .25 objective. I didn't sort the pictures but you can clearly see the photos where the cover was taken off. Again these photos were captured under lower magnifications relative to a Stereoscope. However, Metallurgical scopes are capable of out performing stereoscopes far as, better resolution and higher magnification in many applications. The only thing that sucks about Metallurgical scopes, its strictly dark field. I tried to view a tube tonight that had suspended myc, it took under lighting to even begin to get the suspended myc into focus and the resolution, i would be able to get better quality using my 'Student' AmScope Compound at a the higher magnification for this situation.

Anyway i know its seemed like i was leading up to some special crazy scopy pics, nope......we just getting started with some single strain mycelium views for now...... @needles you better be on your game....well i know your on it......but you better bring it @tenderfoot, get rid of that of that "Inspector Gadget" Captain Crunch secret decoder that you collected after shredding your gums one Saturday morning......it does not work man, it doesn't work man.

*I will have more structure to my posts and photos at some point, such as magnification levels, stains/reagents used, anecdote certain locations, explain what we are seeing and blah..blah for each individual photo.

Attached Thumbnails

  • 20210226_174458.jpg
  • 20210226_174444.jpg
  • 20210226_174423.jpg
  • 20210226_161345_HDR.jpg
  • 20210226_161343.jpg
  • 20210226_160902_HDR.jpg
  • 20210226_160552.jpg
  • 20210226_160532.jpg
  • 20210226_160549.jpg
  • 20210226_160552.jpg

  • sandman, fahtster, Arathu and 6 others like this

#4 Microbe

Microbe

    civis scientiam

  • OG VIP
  • 4,637 posts

Donator


Awards Bar:

Posted 26 February 2021 - 08:15 PM

So today i brought out my Metallurgical scope as it was the easiest accessible, and cleaned the dust off it.......man-alive there was a lot. I put a old plate under the objectives to see what i could capture, and although i was impressed, i was even more so when i ran my objective down too far and cracked my plate cover (very bad for objectives btw). Once cracked, i knew it was compromised, so i just took the cover off and captured some really great low magnification photos.

I need to invest in a quality microscope camera. Im also limited to low magnifications until i get my illumination source and positioning corrected. I can't get light on my specimen using my higher objectives as im using external top lighting via LED A19 bulb in the 3700 spectrum.....horrible illumination source.

The plate was viewed under a 10x ocular and 5x .1 objective, and 10x .25 objective. I didn't sort the pictures but you can clearly see the photos where the cover was taken off. Again these photos were captured under lower magnifications relative to a Stereoscope. However, Metallurgical scopes are capable of out performing stereoscopes far as, better resolution and higher magnification in many applications. The only thing that sucks about Metallurgical scopes, its strictly dark field. I tried to view a tube tonight that had suspended myc, it took under lighting to even begin to get the suspended myc into focus and the resolution, i would be able to get better quality using my 'Student' AmScope Compound at a the higher magnification for this situation.

Anyway i know its seemed like i was leading up to some special crazy scopy pics, nope......we just getting started with some single strain mycelium views for now...... @needles you better be on your game....well i know your on it......but you better bring it @tenderfoot, get rid of that of that "Inspector Gadget" Captain Crunch secret decoder that you collected after shredding your gums one Saturday morning......it does not work man, it doesn't work man.

*I will have more structure to my posts and photos at some point, such as magnification levels, stains/reagents used, anecdote certain locations, explain what we are seeing and blah..blah for each individual photo.

Like i said, photos don't do justice. On my phone, i can zoom in on my photos i took and see pretty damn good detail but again, nothing like seeing it with your own eyes. However, im pretty sure photos are compressed to save space when uploaded. I can't see the tiny water droplets on the photos i uploaded......oh well we going to have some fun anyway.
  • sandman likes this

#5 Microbe

Microbe

    civis scientiam

  • OG VIP
  • 4,637 posts

Donator


Awards Bar:

Posted 26 February 2021 - 08:16 PM

Looking forward to your interesting threads developments!

You have a scope? Come play with me/us sandman.
  • sandman likes this

#6 sandman

sandman

    Saint PP

  • Honorary Former Staff
  • 4,133 posts

Awards Bar:

Posted 26 February 2021 - 08:22 PM

Oh I'm in 

 

Mom says I have to be home when the street lights come on tho

 

edit: maybe crop the photos to just the good bit to keep it from shrinking too much on upload? 

 

Ive got just the thing to take some pics of. Have a plate with some nrmal and some fluff growth lets take a closer look ehh? Never taken a pic thru it before so hopefully they come out.


Edited by sandman, 26 February 2021 - 08:25 PM.

  • Microbe likes this

#7 Myc

Myc

    El Jardinero

  • App Administrator
  • 7,628 posts

Awards Bar:

Posted 26 February 2021 - 08:24 PM

That is an interesting treasure you have there.

 

I'm wildly curious and want the to come across as you describing your latest car purchase. Make, model, year, horsepower............

I know........... I'm sick. Microscopy porn and hot-rod instruments. And I've never seen your particular instrument before.

 

Edit:

You guys type faster than I do ( or more accurately, perhaps ).

I edited questions from my statement having realized that the answers were in a post that came in while I was typing.

Gentlemen, Don't grow old.


Edited by Myc, 26 February 2021 - 08:33 PM.

  • sandman, Arathu, Microbe and 1 other like this

#8 Microbe

Microbe

    civis scientiam

  • OG VIP
  • 4,637 posts

Donator


Awards Bar:

Posted 26 February 2021 - 08:51 PM

Oh I'm in

Mom says I have to be home when the street lights come on tho

edit: maybe crop the photos to just the good bit to keep it from shrinking too much on upload?

Ive got just the thing to take some pics of. Have a plate with some nrmal and some fluff growth lets take a closer look ehh? Never taken a pic thru it before so hopefully they come out.

Good call!

#9 Microbe

Microbe

    civis scientiam

  • OG VIP
  • 4,637 posts

Donator


Awards Bar:

Posted 26 February 2021 - 08:54 PM

That is an interesting treasure you have there.

I'm wildly curious and want the to come across as you describing your latest car purchase. Make, model, year, horsepower............
I know........... I'm sick. Microscopy porn and hot-rod instruments. And I've never seen your particular instrument before.

Edit:
You guys type faster than I do ( or more accurately, perhaps ).
I edited questions from my statement having realized that the answers were in a post that came in while I was typing.
Gentlemen, Don't grow old.

Now that i can't i edit my posts, i use a mobile notepad and then copy and paste. Im a C at grammar at best, that shit took to me like 3 hours to create and post
  • sandman likes this

#10 Microbe

Microbe

    civis scientiam

  • OG VIP
  • 4,637 posts

Donator


Awards Bar:

Posted 26 February 2021 - 09:07 PM

That is an interesting treasure you have there.

I'm wildly curious and want the to come across as you describing your latest car purchase. Make, model, year, horsepower............
I know........... I'm sick. Microscopy porn and hot-rod instruments. And I've never seen your particular instrument before.

Edit:
You guys type faster than I do ( or more accurately, perhaps ).
I edited questions from my statement having realized that the answers were in a post that came in while I was typing.
Gentlemen, Don't grow old.

As far as model, its a professional scope, and is modular as most professional scopes are. As far as i can tell, the body, stage, turret and etc is the Olympus BH3. The oculars are also olympus while the objectives are NEO's. My Metallurgical and Stereoscope are dated, but are professional quality. My AmScope looks fancier, but the two said-above, blow it out of the water at lower magnifications.

Edited by Microbe, 26 February 2021 - 09:08 PM.

  • Myc likes this

#11 rockyfungus

rockyfungus

    Mycotopiate

  • Free Member
  • 1,236 posts

Posted 26 February 2021 - 11:23 PM

Your own eyes got that fucking binocularity...as well as true depth.

Actually, been years but those microscopes are monocular?
I use a stereo microscope that moves in a different plane for work. True binocular views are amazing.

Edit: need to read better...You do have a stereoscope, how are the views with that?

Not a microscope but a really powerful lens used to look at live tissue in different angles.

20.JPG


Edited by rockyfungus, 26 February 2021 - 11:39 PM.

  • Microbe and YoshiTrainer like this

#12 Arathu

Arathu

    Dirtmaker

  • OG VIP
  • 6,829 posts

Awards Bar:

Posted 27 February 2021 - 08:32 AM

Well, I have an AmScope too......cause Needles takes all the good stuff.......hahahahahaha 

 

NO that ain't right, I get my share of the goodies too.......but my allowance gets spent on other things before I manage to get anywhere near buying GOOD glass.....

 

Perhaps another round of stimulus could be directed into some quality instruments.....then again the Harley is going back on the road very shortly.....

 

Still I'm definitely paying attention Microbe...cool thread...

 

A


Edited by Arathu, 27 February 2021 - 08:33 AM.

  • Needles, Microbe and Moonless like this

#13 Microbe

Microbe

    civis scientiam

  • OG VIP
  • 4,637 posts

Donator


Awards Bar:

Posted 27 February 2021 - 11:21 AM

Actually, been years but those microscopes are monocular?

What microscopes? Metallurgical? They can either be monocular, binocular, and even triocular. Mine, the one in the photo, has 2 oculars, so binocular. All three of my scopes are binocular, you can get greater magnification with clearer resolution.

Easiest way to tell, is simply count the oculars.

Im not sure i understand your question entirely so perhaps i did not answer your question. Let me know.

Oh and yes i have a stereoscope. The images are bad ass. Great for viewing 3d objects. The metallurgical is a very powerful scope, and far better at higher magnifications. I might dig my sterescope out today. Its a relic also but its what you see through the lense is what matters. Lol

Edited by Microbe, 27 February 2021 - 11:26 AM.


#14 Microbe

Microbe

    civis scientiam

  • OG VIP
  • 4,637 posts

Donator


Awards Bar:

Posted 27 February 2021 - 11:55 AM

Well, I have an AmScope too......cause Needles takes all the good stuff.......hahahahahaha

NO that ain't right, I get my share of the goodies too.......but my allowance gets spent on other things before I manage to get anywhere near buying GOOD glass.....

Perhaps another round of stimulus could be directed into some quality instruments.....then again the Harley is going back on the road very shortly.....

Still I'm definitely paying attention Microbe...cool thread...

A

AmScope makes some high end models for sure. I fell for the MSRP 1400.00 get it now for only 300. I impulsively clicked buy now. Turns out, my compound scope is not a 1400 scope. However it gets the job done and i can upgrade the objectives and oculars to pro glass. The light source is shit also but i can supplement that with crap i have laying around the house. I might dig that one out today also. It is by far my least favorite scope to use because of all the steps in slide prep and trying not to contaminate the specimen but really is cool once you get down in to the world of bacteria. I scrubbed my entire body 3 times a day after looking through one of those for the first time. I swirled my hand around is some distilled water and a way i went. I was soon saying what the fuck is that? That fucker is living on me? And to the shower i went with my antimicrobial soap. I accept it now

My end game boss level, is getting a SEM scope but i need to pay off all my debt before i do that or my wife will divorce me.......shit she still may but i will develop a nee relationship with my SEM

Im pretty sure Needles has been putting together a SEM as he salvages parts from PSU. I may be wrong but he has a great contact there. Im kind of jealous really

Edited by Microbe, 27 February 2021 - 11:56 AM.

  • Arathu likes this

#15 rockyfungus

rockyfungus

    Mycotopiate

  • Free Member
  • 1,236 posts

Posted 27 February 2021 - 12:34 PM

Well technically in some microscopes only one eyepiece is focus able. Or has magnification even...it's been years since I've used a classic microscope.

"A stereo microscope provides a 3D image and a Binocular flat field microscope provides a flat image. Please know that having two eyepieces does not make a stereo microscope.

A stereo microscope utilizes two separate optical paths, in other words there is a separate “microscope” for each eye. These optical paths are inclined at a six degree angle towards each other so that the user can see a portion of the sides of the specimen as well as the top. This separation of views allows us to perceive depth and see in true 3D."

The lenses I showcased earlier is a double aspheric lenses, by moving a light source, the angle of the lenses you can visualize on various planes. Focus on the grain, focus on the myc, focus on the interface between the two.


  • Microbe and YoshiTrainer like this

#16 Microbe

Microbe

    civis scientiam

  • OG VIP
  • 4,637 posts

Donator


Awards Bar:

Posted 27 February 2021 - 06:34 PM

Right of the rip im editing!

EDIT: can i not add photos inline anymore? This post is going to be a trainwreck. I provided a description and or comment then i added the photos below by clicking add to post, typed some more info, added photos....rinse repeat. But it compiled all my photos at the bottom of the post. Damn it man that is so criminal. I actually tried to make a structured post and bam its whack.

So i brought out my old American Optical Binocular Stereo Star microscope model #569, 10x ocular s 0.7x to 3x. There was so much dust it and i need to get inside this one and clean it. Its old and corroded but it serves its purpose. Im using top lighting and deflecting off the bottom mirror lense. This where a stereo microscope can do, what the metallurgical scope can not.

I can't stress this enough, these pictures simply do not serve justice of what im actually seeing when i look through the glass. For those of you who have uses microscopes, would certainly agree.

Not sure why my photos are turning. I had this problem before. Here is my Stereo Microscope. She is a little ugly but works.

20210227_133459.jpg
20210227_133509.jpg

Some photos of a colonized agar plate with the cover on. The first few pics are some odd growth. I eventually sacrificed the plate by taking the cover off to see what the hell was going on. More on that in a minute.

20210227_141717(1)(1).jpg
20210227_141717(1).jpg

A few Rhizo shots. Keep in mind, this culture is a year old.....on a plate and in cold storage so it doesn't look the best i assure you, it's still agressive in grains and does it thing. I took a few plugs from it about a month ago.

20210227_140654(1).jpg
20210227_140555(1).jpg

Agar plug that brought the life to this plate.

20210227_135546(2).jpg

Some primordial action.

20210227_134914(1).jpg
20210227_134558(1).jpg

Cover comes off. Couldn't get a good shot this time for some reason so i gave up but uere is that weird growth pattern again. It looked as if baby spiders should come crawling out.

20210227_143228(1).jpg

Mystery solved. What in the F is a oat doing in this plate along with a agar plug? Im baffled and i can't answer that. Perhaps the mycelium replicated its DNA, built a cocoon like structure, and created that single oat grain. I have no idea how TF i did that.

20210227_143747(1).jpg
20210227_143617(1).jpg
20210227_143727(1).jpg

Some more rhizo and some mycelium i rolled of the agar surface. It kind of felt like eraser debris and yes i felt bad after doing it. Oh and i dropped the oat by it to make it even more weird.

20210227_143443(1).jpg
20210227_143547(1).jpg
20210227_143844(1).jpg

Some bad ass shots of some condensation. Almost looks metallic.

20210227_140357(1).jpg
20210227_140356.jpg
20210227_140321.jpg
20210227_140256.jpg

This is what i was unable to view with my Metallurgical. Crapy photos of some suspensed mycelium.


20210227_140126(1).jpg
20210227_140119(1).jpg
20210227_135936(1).jpg
20210227_135801(1).jpg

A little bit of poly fibers and it is neat how much it resembles Hyphae strands.

20210227_144806(1).jpg
20210227_144808(1).jpg
20210227_144848(1).jpg

A cannabis leaf. I didn't really try hard on the leaf itself, but was more interested it the stem which i split open.

20210227_145117(1).jpg

20210227_144940(1).jpg
20210227_145213(1).jpg

Some colonized sub i yanked from a bag. It looks kind of gross. It is straight coir which is very unusual for me.

20210227_145450(1).jpg

20210227_145508(1).jpg
20210227_145539(1).jpg
20210227_145618(1).jpg
20210227_145700(1).jpg

Some cat fur from my orange Tabby.

20210227_145913(1).jpg

20210227_145924_HDR(1).jpg

That's it for this post. I am never ever doing another post like this from my phone again. Taptalk use to be awesome for me but now it does not work for uploading photos. Im ordering a camera for my scopes asap after speaking with @needles......it makes me slightly ill not beinng able to really show what im seeing for those whom may never have looked through a microscope.

Anyway im playing around a bit but the real neat stuff is coming soon.

Edited by Microbe, 27 February 2021 - 06:43 PM.

  • sandman, Needles, pastyoureyes and 2 others like this

#17 rockyfungus

rockyfungus

    Mycotopiate

  • Free Member
  • 1,236 posts

Posted 27 February 2021 - 08:01 PM

There's attachments for the phones now a days for the oculars. I've made a simple one for my job with some plexiglass.

https://www.amazon.c...ope phone mount


  • Microbe and ElPirana like this

#18 bezevo

bezevo

    Mycotopiate

  • Gold VIP
  • 1,152 posts

Awards Bar:

Posted 27 February 2021 - 08:36 PM

My Microscope  is Junk .. i need to find one simple enough for me to use without fucking it up ...  but good enough to see spores ect and perhaps view on a CrapTop  and capture clear images ..  or use with a phone . i will have to  concentrate and read this thread see what i can learn heh

Thanks Microbe

 

 

i'm not sure if i'm smart enough for a smart  phone

 

BEZ


Edited by bezevo, 27 February 2021 - 08:45 PM.

  • Microbe likes this

#19 Microbe

Microbe

    civis scientiam

  • OG VIP
  • 4,637 posts

Donator


Awards Bar:

Posted 27 February 2021 - 08:43 PM

My Microscope is Junk .. i need to find one simple enough for me to use without fuckinng it up ... but good enough to see spores ect and perhaps view on a CrapTop and capture clear images .. or use with a phone . i will havve to concintrate and read this thread see what i can learn hheh


i'm not sure if i'm smart enough for a smart phone

BEZ

What kind of scope do you have? You say junk, what's wrong with? I might be able to help you troubleshoot it.

#20 bezevo

bezevo

    Mycotopiate

  • Gold VIP
  • 1,152 posts

Awards Bar:

Posted 27 February 2021 - 08:51 PM

My microscope is for a beginner/ student ....known as a  Student microscope ....heh..... Just a step up from a toy .

  i want to up grade   .. a bit more power and the ability to link to a  laptop or phone without having to be to geekyy to get it to pair up and work ,i'm old guy not so good with tek /software ect.

 

Prices seem to be  $150 to $350 ..  some the software is a bit beyond me it seems .


Edited by bezevo, 27 February 2021 - 08:55 PM.

  • Microbe likes this




Like Mycotopia? Become a member today!