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Microscopy With Microbe & Friends


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#41 Needles

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Posted 19 March 2021 - 07:56 PM

But lucky for me I have old technology on hand and ready to bring you time laps photography of a coenocytic hyphae fugle cell reproducing....

??? will there be symbiotic bacteria involved???

subscribe for results......


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#42 rockyfungus

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Posted 19 March 2021 - 08:02 PM

Is there a stain you use for photography or viewing spores? Or most are fine without and stains are more of a gradient or for picking up other things (gram stain etc)



#43 Microbe

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Posted 24 March 2021 - 05:49 AM

Is there a stain you use for photography or viewing spores? Or most are fine without and stains are more of a gradient or for picking up other things (gram stain etc)

It really depends on the color of the spores to which stains and or reagents to use. However, lacto-phenol cotton blue is a general all purpose stain. This will stain the outer structure of the spore blue allowing for a more defined shape. LCB is excellent for hyphae structures as well. There are better stains for spore staining, but again, you will have more applications going with LCB. Keep in mind sores can easily be observed when mounted using only distilled water.
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#44 Microbe

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Posted 24 March 2021 - 05:51 AM

Sorry i have not updated this thread, im on a pipeline and its a grind 6 days a week. I don't even have time to go to Pet Smart and punch puppies in the face

Joking about the puppies, i care more for animals then i do people. Facts and truth!
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#45 bezevo

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Posted 24 March 2021 - 12:41 PM

I REALLY  need a $200 tp $ 300 scope i can Tye to a CrapTop to view and capture images . something not to complex for the simple minded ha .. i am probably  dreaming . 

i read descriptions for hours and still don't have a clue ....

man ,,not feeling to smart in this area.

 

the Electron microscope is cool .. so is my Government surplus Alien flying  saucer i bought at a DOD auction in New Mexico .

anyone know much about repairing Alien anti Grav Emitters ?

 

BEZ



#46 Microbe

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Posted 24 March 2021 - 06:41 PM

I REALLY need a $200 tp $ 300 scope i can Tye to a CrapTop to view and capture images . something not to complex for the simple minded ha .. i am probably dreaming .
i read descriptions for hours and still don't have a clue ....
man ,,not feeling to smart in this area.

the Electron microscope is cool .. so is my Government surplus Alien flying saucer i bought at a DOD auction in New Mexico .
anyone know much about repairing Alien anti Grav Emitters ?

BEZ

Ask @TVcasualty......he always has the answer, but also articulates it, using the 'Queens English' grammar.
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#47 Microbe

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Posted 01 June 2021 - 08:56 AM

Light-shrinking material lets ordinary microscope see in super resolution

by University of California - San Diego


https://phys.org/new...cope-super.html
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#48 rockyfungus

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Posted 01 June 2021 - 11:56 AM

https://www.nature.c...467-021-21835-8

 

"We deposit a thin SiO2 protection layer (~10 nm) with minimal defects on top of HMM before cell growth to not only increase the biocompatibility of the HMM substrate but also reduce the quenching effect of the fluorescence dyes."

 

"Moreover, speckle-MAIN does not rely on any specific properties from fluorescent molecules, so that any fluorescent probes can be used. The experiment can be conducted under a conventional epi-fluorescent microscope, with an HMM-coated substrate and a multi-mode fiber for illumination."

Glad to know you can use multiple fluorescing agents. 

 

 

So different from papers I've had to use academically. When did they start doing this? Or just some journals?

https://static-conte..._MOESM3_ESM.pdf

https://static-conte..._MOESM2_ESM.pdf


Edited by rockyfungus, 01 June 2021 - 11:57 AM.

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#49 Needles

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Posted 16 November 2021 - 06:11 PM

post-136520-0-61871900-1637104117.jpg

I'm trying to get the dust off my scopes and start back at it. For starters, a shot from a prepared slide, using a lwd CDPlan 20PL 0.40 objective its a pinus young male strobilus lol its a pine tree....

 

 

 

 

pine tree.jpg


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#50 Arathu

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Posted 16 November 2021 - 07:36 PM

Dude that's awesome stuff right there.......trying.....hah!

 

A


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#51 rockyfungus

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Posted 16 November 2021 - 08:15 PM

That's a textbook plant slide.
Nice cell wall, chloroplasts, and organelles!


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#52 Needles

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Posted 17 November 2021 - 11:41 AM

post-136520-0-14376600-1637166444.jpg

 

 

View using a 5x objective

 

post-136520-0-83407100-1637166569.jpg

 

View using a 10x objective

 

 

post-136520-0-16624800-1637166713.jpg

 

View using a 20x objective

 

post-136520-0-14162000-1637166844.jpg

 

View using a 40x objective

 

post-136520-0-90150100-1637166978.jpg

 

Native mushrooms from Pennsylvania

 

 

These micrographs will give you an idea what to expect a sample to look like at different magnifications. spores are starting to be able to be identified at 40x.  For this study I used a Olympus inverted tissue culture microscope. Subject of interest is a Coprinus cross section, once again a prepared slide. I am including a photo of a mushroom I took a pix of last week. Later I will post images of the same sample only using a parfocal microscope with a 50x and 100x oil immersion objectives.

 

 

 

 

 

 

 

 

 

 

 

inkweb.jpg

40xweb.jpg

20xweb.jpg

10xweb.jpg

5xweb.jpg


Edited by Needles, 17 November 2021 - 11:48 AM.

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#53 Needles

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Posted 17 November 2021 - 10:55 PM

Concluding the Coprinus photo study with two more micrographs. This will show how the more powerful 50x and 100x oil immersion objectives are very useful when it comes to studying the morphology of fungal spores. Hope this helps anyone that may be considering investing in microscope equipment to explore the mycology hobby..

 

 

post-136520-0-09490700-1637207375.jpg

 

View using a 50x oil immersion objective

 

 

post-136520-0-12602200-1637207586.jpg

 

View using a 100x oil immersion objective

 

 

100_oilweb.jpg

50_oilweb.jpg


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#54 Arathu

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Posted 18 November 2021 - 05:00 AM

I will run clean out of likes before sun up......hahahahaha

 

Needles has SERIOUS skills.............beautiful and very informative work brother.

 

Did you use a stain on these or is that natural color? Very cool....

 

A

 

EDIT: I see it comes from a prepared slide.....still very cool.....I have to order some more immersion oil...


Edited by Arathu, 18 November 2021 - 05:03 AM.

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#55 Myc

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Posted 19 November 2021 - 10:55 AM

A tutorial on sample prep would be really useful. I have never prepared a slide like the one featured above and it would be fun to have the skills.


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