Here's an easy tek for jar lids you can use for spore water, clones, grain jars, liquid cultures, and anything else you can think to do with them. They're made from very basic, easy to obtain materials.
SHIP stands for "self-healing injection port", which means that you can inject a needle and withdraw it, and the port will automatically seal back up without needing to do anything more. They're "breathing" because air can flow in and out of the jars without introducing contamination to the contents. This is important to prevent pressure differences that occur if you pressure cook a totally sealed jar. The breathing part is important, because if you pressure cook a sealed jar, it creates a pressure difference between the inside of the jar and the outside air. That pressure difference is a problem because it can do things like suck an entire syringe's worth of liquid into the jar, when you only wanted to inject a 1/2 cc.
Here's what a finished lid looks like:
This isn't a new idea. I got it from Buckaroo Banzai years ago. Here's the thread I probably picked it up from, in 2006: https://mycotopia.ne...s-silicon-lids/. Unfortunately, the pictures are long gone from that thread.
Here are the materials you'll need:
Lids, washers, tyvek envelopes, silicone, hammer, and screwdriver. Also a pair of scissors and some paper towel. The silicone is aquarium sealant, but I've used various types of 100% silicone over the years. Yes, that's a priority mail envelope from the good ole' post office. Those envelopes are made from tyvek, which is a good material for the air filter. I'm going to use pint jars here, which I like for holding spore water and liquid cultures.
1. Punch two holes in the lid using the hammer and screwdriver.
I screw the lid right onto a jar while doing this step, because the jar holds the lid for you. I'm sure there are better ways to punch holes in lids, but this is the easiest way I know. Be careful not to break the jar! Put a towel under the jar and use a good solid surface under the towel. Carefully hammer the screwdriver through the lid to make two holes. One will be for the SHIP and one will be for the filter.
The undersides of the holes will be jagged. You can flatten the metal down by putting the lid on the towel and pushing down with the butt of the screwdriver.
2. Cut circles of tyvek the size of your washers.
Use one of your washers to trace circles in the tyvek envelope. You'll need two circles of tyvek per jar lid.
3. With the lid screwed onto the jar, use silicone to create the SHIP and the first half of the filter.
One of the holes in the lid will be for the SHIP and the other will be for the filter. With the lid screwed on the jar, put a good glob of silicone over the hole closer to the edge of the lid. Draw a bead of silicone around the other hole and place a washer over the hole, being careful not to seal the hole with silicone. A chopstick is a good tool to help you here--you can use it to keep the hole clear.
Then, draw another bead around the top of the washer, and carefully place a tyvek circle on the washer, making sure to contact the silicone all the way around so it's sealed.
4. Unscrew the lid, turn it over, screw it back down, and do the same on the other side of the lid.
Repeat step 3. for the other side of the lid. Doing this creates a space between the two tyvek filters, which allows the bottom one to get wet while protecting the top one. This prevents the filters from becoming a vector for contamination.
5. Allow the lid to set for 24-48 hours before using.
Once the lid is finished, you can pressure cook it all you want. You can jab the silicone glob (SHIP) many times for many projects before it needs replacing. If you do need to replace it, use a razor blade to cut it off and put a new glob of silicone on there.
For spore water, I'll fill a pint jar halfway with filtered water, screw on one of these lids, cover with foil, and PC for 30 minutes along with some empty syringes. Then, under sterile conditions, I'll draw up water from the jar into a syringe, shoot it into the button bag containing the spore print, draw it back up, and shoot it back into the jar.
For liquid culture, do the same thing but fill the jar with your liquid culture broth before PCing. Then you can put your spores or tissue into the jar for incubation.
Here are some other lid teks you can look at:
https://mycotopia.ne...e-test-of-time/ - Lids that have stood the test of time
https://mycotopia.ne...06-jar-lid-tek/ - Jar lid tek
https://mycotopia.ne...-tyvek-jar-tek/ - Replaceable tyvek jar tek
https://mycotopia.ne...tches/?p=160899 - Fahts self-healing lids and bag patches
https://mycotopia.ne...iquid-cultures/ - Vanilla extract and vented lid tek for liquid cultures
Edited by Sidestreet, 04 April 2021 - 09:06 AM.