No Pour Plates Ziran Style
This is a simple a straight up way to make no pour plates. Using something very similar to pasty plates.
No drills no micropore tape. All you need is the containers, agar, potato flakes, some honey, sugar or karo.
The recipe! Thanks to munch for it
Potato Dextrose Agar & Pasty Plate Preperation
For 8-10 Pasty Plates You Will Need:
- 1 gram honey or raw sugar
- 2 grams agar
- 3 grams potato flakes (plain instant mashed potatos)
- 1/2 cup water
- optional: food coloring
- Set your stove-top to high, then combine all ingredients in a pot. Use the hot water to help dissolve the honey.
- Let the mixture reach a boil
- Upon boiling, immediately remove and pour into a measuring cup. You can use the cup to see how close to 1/2 cup you are. if you cooked off too much water, you can simply add some back in.
I simply pour my agar into these ziplock round containers. Also found some neat ones that are about as big as the 4oz jelly jars.
Then after the pour is complete, simply screw the lids on tight, and do about a 1/4 to a half of a turn to loosen it up. It shouldn't come off but will be on tight enough not to melt the plastic. Just like in the gif below.
After this is complete, cover the lids in tinfoil like so
and into the pc they go for 30 minutes at 15 psi.
After a few transfers, you'll end up with plates like this.
Made 2nd Transfer
Made 3rd Transfer
And some expansion.
And here are some clean and ready to use cultures!
I have found so far that with the bigger containers it is easier to start a culture/clone in the big containers and transfer to smaller containers like so. This plate is from my BFM clone I started. It is just so much easier to transfer away from contamns as there is so much more surface area compared to the small 4 oz jelly jars.
And the new plates!
3rd transfer plates
A king oyster master in my newer plates
Edited by Ziran, 05 April 2021 - 04:37 AM.