Getting my project off the ground has proven troublesome - just from a lack of time. I just finished my first set of jar lids today, and within a day or two, I'll be running the PC. For the remainder of the post, everything is multispore inoculation.
I'm trying to remember how I used to sterilize PF cakes. I'm pretty certain my PC options are 5, 10, 15 lbs, so I think I went 60 minutes @ 15 psi. Does that sound fine?
As far as LC, I'm going to compare both honey and LME. As far as PC, I know I've got to watch my sugars here - will 15 minutes @ 5 psi give a good result? Will 15 minutes @ 10 psi caramelize? I do like to be certain I'm getting the media sterile. These jars will have double SHIPs (one to release the vacuum and the other to inoculate).
For agar, I'm planning on going 30 min @ 15 psi. I was hoping to find half pint wide mouth mason jars, but that is apparently laughable in April. I heard Ball had a big sales year last year too, what with COVID and all. Anyway all that to say - I've got a collection of small #5 plastic containers, which I'm under the impression will hold up in the PC? Just hoping to confirm that before I ruin my PC. I do have a few dozen half pint mason jars, but they won't last long with all the stuff I'm going to start. I'd like to make the plastics work. If #5 plastic IS acceptable for the PC, can I put the lids on tightly to PC and will I be able to easily remove them in my SAB, which will have plenty of mobility?
And finally for rye, 60 min @ 15 psi.
Does that all sound good? Last question is - I clearly have a lot of PC work ahead of me. I know that it's ideal to inoculate as quickly as possible to give the spores a chance to beat any baddies. How long is too long to wait - I think I've probably got many PC runs that I'll have to do - and the bad part is that for any given syringe, I'll be using each run (in most cases): LC, agar, PF, and grain.
Many thanks for any help.
Edited by SelfTransformingMachineElf, 05 April 2021 - 08:04 PM.