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About to embark on my first agar adventure.


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#21 Oldpunk

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Posted 21 April 2021 - 06:59 AM

Since I'm awake at the buttcrack of dawn I tried to get clearer pics.

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#22 Oldpunk

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Posted 22 April 2021 - 07:15 PM

Day 8. My spots from spores are starting to collide. I'm thinking I should take some samples out for new plates before they grow together any further.

Would you recommend taking samples from the smaller spots? Or just stick to the main one.

My furnace went out and it was only 50 degrees in here. That's when that blue ring showed. Once the temp was back to high 60s the myc started growing again.

Pretty cool.

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#23 Oldpunk

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Posted 22 April 2021 - 07:24 PM

Then my 2nd question for y'all.

This one is from a tissue sample. So it's already an isolated strain?.

It's about the size of a quarter. And im thinking I could refrigerate this and use a chunk to test a grain jar.

Or is my thinking faulty on that?
Does a person transfer a piece to a clean plate and grow that one out for long term storage? That would make some sense to get rid of the clone material and just save the mycellium.

Thoughts?

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#24 rockyfungus

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Posted 23 April 2021 - 06:42 PM

That's fuzzier then I'm used to. If you are confident that the plate is clean. Just squeeze the plastic and let the whole puck drop into some grain. 

I always make one more transfer just as a failsafe. Then once I print my fruits or I'm happy with the grain jars progress. I'll either discard that agar, fridge it for 1/2 a year or so (inside a bag or something to help from fridge cycles/door opening), or I'll put it to an agar slant for even longer term storage. 



#25 Oldpunk

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Posted 24 April 2021 - 03:06 PM

That's fuzzier then I'm used to.

Right?
All of my first samples are super fuzzy growth. Maybe a little strong on the sugar? I used potato flakes and karo. If you've ever tried measuring 1g of corn syrup without getting it all over your scale, tools, yourself.....then you may know it's hard to be precise on that one.

So a couple days later here's a few more pics.
Out of 3 plates only one spot from the Tampanensis.

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And the AA+ is really whispy and the only one not real fuzzy.

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This Cambodia clone is still a furry beast.

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And 2 of my first transfers after about 36 hrs. I pulled 7 different pieces from the KSSS plates.

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And last but not least....ok last and least. Couple of contamination pics. One is another clone from a small cap that looked like a nipple....so I had to try. The other is a spot on a new plate so musta been something during the pour or in the cup.

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I picked up some malt extract so I can try these again on a different medium next time. Will probably use LC for all next time
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#26 Oldpunk

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Posted 28 April 2021 - 01:33 AM

Almost 2 weeks and I'm gonna try to make a couple more transfers and see how a grain jar starts from agar.

Earlier today I saw it commented in another post that too much nutrient would make the plates grow extra fuzzy. Im definitely having that issue. So would my mix be too heavy on the potato flake? Or the corn syrup?

I'm gonna make transfers to my remaining PDA cups and then mix a batch using malt extract for next round.

After 13 days I've got this hairy beast of a Cambodian clone that I'm gonna use to start a grain jar.

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A very whispy Albino A+ sample.

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A fuzzy Tampanensis

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And a half dozen samples from Koh Samui

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About half of these first substrain transfers look like they are growing more like I want them to so I'm hoping if I make some transfers from the others I will start seeing less fuzzy growth and more of the ropey mycellium we're after.

One way to find out. And if it's mostly the agar being mixed wrong then hopefully the next batch will be more on cue. Any suggestions on recipe changes? Think I did a 3potato-2agar-1corn syrup ratio.
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#27 rockyfungus

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Posted 28 April 2021 - 06:49 AM

I really like using my grain water from cooking my grains. I dilute the left over boiled water 4x and then add the agar for the volume of liquid. 

Most people use malt extract for their agar/LC. I don't see any wild performance differences between my grain water agar and malt. 



#28 Oldpunk

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Posted 30 April 2021 - 05:39 PM

Made another round of transfers. Decided to try agar/grain. And made another batch of agar cups. Need to be careful how many new jars and plates I start at once. Easy to get too many going all at once.

These first ones are looking pretty good.
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Multitasking in the PC
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And I ended up with 3 grain jars and some new plates of the AA+ and tampanensis.
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And then I poured new cups for the next rounds and maybe some new starts from prints. I decided to do one more try with the potato flakes and corn syrup, but I cut my mix down to 8g potato 8g agar and 2.5g corn syrup. To 400ml water.

Hoping the new mix will cut down on the super fuzzy growth.
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#29 Arathu

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Posted 30 April 2021 - 08:05 PM

Very nice work man......looking good......perhaps try a batch of the same and add just a small amount of honey to the mix.....

 

Giving the grains agar shots......

 

A



#30 Oldpunk

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Posted 01 May 2021 - 10:48 AM

Very nice work man......looking good......perhaps try a batch of the same and add just a small amount of honey to the mix.....
 
Giving the grains agar shots......
 
A


Hey thanks. I actually read through your agar start in the vaults. I've soaked up a lot of that info and used it as a reference to how I look at my own results. So the comment kinda feels like a pat on the back from a (estranged and unknowing) teacher.

#31 Oldpunk

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Posted 03 May 2021 - 04:48 PM

Today I decided to make a couple of transfers to the new lighter agar mix. I used the same "mother" plates as the last transfer so I should get a good comparison once they grow out a bit. The first round is growing out very thick and fuzzy. Although some of them look pretty good and I can definitely tell the different zones growing out from seperate substrains.

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That last one is the Albino A+. It's seems to be growing much better after a transfer. The plates from spore were very thin and whispy.

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These were the first KSSS samples

And I checked on the grain jars which seem pretty happy with their agar infusion.

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#32 Oldpunk

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Posted 04 May 2021 - 02:17 PM

Purely out of curiosity.

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It was this or the trash.
Unpasteurized top soil.

#33 Arathu

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Posted 04 May 2021 - 04:51 PM

Woodies LOVE topsoil too......and cubies grow crazy large and potent in outdoor "beds" and fake HPOO/CPOO "patties".......permanently shady spots, especially on the north side of something significant (building, large rocks, block wall, and etc)

 

Kept damp and with some green cover......OH MY....  :biggrin: 

 

I love to experiment with things too.......good stuff man!

 

A


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#34 cflashor

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Posted 04 May 2021 - 07:28 PM

How are you sterilizing those cups?  Or are you just raw-dogging it?


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#35 Oldpunk

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Posted 05 May 2021 - 07:54 AM

How are you sterilizing those cups?  Or are you just raw-dogging it?


The agar cups?
I'm using the cups straight from the package and pouring over my oven. So far out of 60 cups only 1 has shown contamination.

#36 cflashor

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Posted 05 May 2021 - 05:25 PM

How are you sterilizing those cups?  Or are you just raw-dogging it?


The agar cups?
I'm using the cups straight from the package and pouring over my oven. So far out of 60 cups only 1 has shown contamination.

Good deal, that's a good batting average.

#37 sandman

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Posted 05 May 2021 - 08:42 PM

Guys please we can not say nothing about this person pouring plates over an oven like it's 1999...

 

This is really outdated tek, don't do this. Your cultures do not look good because the agar probably has latent bacteria or other microbes in it. Try no-pour style teks please. 


Edited by sandman, 05 May 2021 - 08:47 PM.

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#38 Sidestreet

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Posted 06 May 2021 - 03:51 AM

Sounds crazy to me too.  Let's see what happens as those grains colonize and fruit, though.  How them grain jars lookin today, Oldpunk?



#39 Arathu

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Posted 06 May 2021 - 05:11 AM

I know it's tough to pull off, especially in unprecedented times, but a PC will change your hobby forever.......

 

No pour is a very good option (although I was converted to pitri dishes and never looked back) and works well plus the jelly jars are reusable......

 

A



#40 Oldpunk

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Posted 06 May 2021 - 08:24 AM

Guys please we can not say nothing about this person pouring plates over an oven like it's 1999...
 
This is really outdated tek, don't do this. Your cultures do not look good because the agar probably has latent bacteria or other microbes in it. Try no-pour style teks please.

Oh man. There was this wicked cool house party new years Eve 1999. So many people jumping around the floors of the farmhouse were flexing and creaking.
Ah the good ol days....just like when they used to pour their agar over an open stove. All analog style.

I like my ghetto cups. You don't have to. I guarantee I will try other methods in time, but this is what I tried first.

Sounds crazy to me too.  Let's see what happens as those grains colonize and fruit, though.  How them grain jars lookin today, Oldpunk?


Can't take a good pic today of the jars today, they were 50% colonized so I gave em a shake last night. They don't look like much right now.

Last night I ran a dozen jelly jars through the PC about 1/3 full of grains. Got an even crazier idea than oven pours in mind for another agar experiment.

Y'all are gonna love to hate it!




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