Once upon a time, I had *really* good luck with LC - I think I was using light corn syrup then.
I got my ratio right (half jars w/ LME, half jars with honey), but after 8 days, there's not a bit of growth! Same spore syringes have all germinated on other media - some PF, some rye grain, some agar. My temperature was really low - I bumped it up a few days ago. The only other thing I can think of is that I'm using 2 SHIPs and no air - although I believe there should be plenty in the jar to get the LC started.
The only LC that DID work is from another LC (purchased). The growth in the LC looks good, but there's also a cottony white something growing on the top. I suppose it's a contaminant - maybe I can get some pictures.
I put a heater and humidifier in the room, but the humidifier quickly ran out of water, and the heater got the room significantly too hot. So I'm a tad worried about my grain jars.
I have material enough to rebuild, I think. But I sure do like the ease of LC. I guess I'll just try some of my agar into LC as a back-up, and then if I get nice sectoring in a few agar transfers I'll try another LC.
Edited by SelfTransformingMachineElf, 21 April 2021 - 10:16 AM.