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My agar trials - Quest for rizomorphic


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#181 rockyfungus

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Posted 22 July 2021 - 08:59 AM

Pick one source of citric/ascorbic acid, don't use them all. 



#182 rhapsodyclaymore

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Posted 22 July 2021 - 10:38 AM

Pick one source of citric/ascorbic acid, don't use them all. 

So, like lemon concentrate? Natural, unsweetened?

 

Also, here's a pic of the day. Massive growth between today and yesterday.

1.JPG



#183 rhapsodyclaymore

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Posted 23 July 2021 - 01:40 AM

Woah, now there's some more massive growth. More pins getting bigger too!

 

1.JPG


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#184 rockyfungus

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Posted 23 July 2021 - 08:17 PM

I used to only use lemon or lime whatever was on hand (little plastic container or fresh fruit). Since, I tried straight ascorbic acid (version of vitamin C), there's no reason for "lemon" tek

Cracks give you sometimes a nice little micro-climate for a bunch of pins growing all over. 


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#185 rhapsodyclaymore

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Posted 24 July 2021 - 05:27 AM

I used this guide which.... appears to be working very well... https://www.reddit.c...potency_no_bad/

2.6g dried were used. Placed into a plastic bag (clean), and crushed up with a rolling pin. Then added to the directions.

Used an electric kettle to heat the water up, let sit for 10+ seconds, then poured into a tea cup combination with a strainer metal part inside and added some loose tea to it for flavor. Let sit for 10 minutes, then removed the inner strainer part, used a tea spoon to press out most of the liquid into the cup, then added some milk to cool it down. Drank it down in 20 minutes.

Made a 2nd cup which is sitting right now and probably has passed the 10 minute mark, but I'm losing track of time. I will finish this post and prepare the 2nd cup to sip on.

The bonus of this was that I could also have a protein rich breakfast (no carbs, sugars, etc) to compliment the tea without (so it seems) loss of potency.

Edited by rhapsodyclaymore, 24 July 2021 - 05:32 AM.

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#186 rhapsodyclaymore

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Posted 24 July 2021 - 02:19 PM

I harvested a couple handfuls today of the 2nd flush. Totaling about 140g wet. There's a lot more that's not mature yet, so I'll give them a day or 2 and maybe run a dehydrator cycle on Monday. The fresh picked ones are in a plastic ziplock baggy with a paper towel inside to keep them dry.


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#187 rhapsodyclaymore

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Posted 25 July 2021 - 04:09 AM

OK, a lot more are now mature and have been picked. There's some smaller ones still that could use another day or two. I've decided to run a dehydrator cycle on what I have right now, totaling around 280.6g wet. The original was just over 350g on the first flush, so this is really not bad at all I'd say.

 

There's not as many side pins in this batch thankfully and I was able to get all the mature ones in the gap between the substrate and the wall of the tub.

 

There's still a massive amount of pins on the surface that are coming up, so I think I will blend the 2nd flush into the 3rd flush in a way and take it from there.

 

After draining the water from the 1st flush, I left a lot in at the bottom to make it not so dry and helpful for side pins to form as easy. This seemed to have worked as any side pins are above the water line.


Edited by rhapsodyclaymore, 25 July 2021 - 05:30 AM.

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#188 rhapsodyclaymore

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Posted 26 July 2021 - 01:33 AM

Ok, dry weight this time was 24g exactly from the partial 2nd flush. Total remaining from my supply is 52.4g including all flushes. Some more mushrooms are starting to mature in the box so I'll keep an eye on them. More pins are coming up. Could be a blended 3rd flush happening.



#189 Oldpunk

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Posted 26 July 2021 - 09:29 AM

This was your first grow? I gotta say you did much better than most people. Myself included.

Now you get to sit back and enjoy. I've found a that few g made into tea makes for a good buzz if your just looking to chill. I tried out just under 5g of a new grow the other night and tried to watch a movie. Too much to focus.
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#190 rhapsodyclaymore

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Posted 26 July 2021 - 09:39 AM

This was your first grow? I gotta say you did much better than most people. Myself included.

Now you get to sit back and enjoy. I've found a that few g made into tea makes for a good buzz if your just looking to chill. I tried out just under 5g of a new grow the other night and tried to watch a movie. Too much to focus.

This is 100% my first grow I've ever done, and I don't have a "green thumb" too. Everything I acquired was from reading as much as I could before creating an account here to ask for help.

The 2.6g dried I made was great to watch netflix. I could probably get to 3g next time tho. I'm looking for that same buzz I had before on truffles and acid where movies are just trippy. The 2.6g was a nice time to relax in the afternoon and not need to care about anything. Totally was worth it!

Today I've just picked a few more mature ones that should get picked. Total about 87.7g wet. I've got them in a baggy in the fridge wrapped (both sides) in a paper towel until a couple more mature and then I'll do another 20 hour 70c dehydrator cycle again. Should dry down to maybe 8g which is not too bad.

My next home project (now that I have proper home lab equipment) is to grow some Peyote. It takes 3 years until harvest, and like the mushrooms, I've been putting it off for too many years. I have a pack of seeds already, I just need to get some sand. The process is relatively the same requiring sterile sand/verm combo to successfully grow. I just need to figure out potting size and some other parts.

Ideally, in the future, it might be cool to have a small psychedelic garden.

Edited by rhapsodyclaymore, 26 July 2021 - 11:14 AM.

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#191 Oldpunk

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Posted 27 July 2021 - 10:24 PM

Heard that. I'm thinkin along the same wavelength.

Congratulation on the grow.

#192 CatsAndBats

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Posted 27 July 2021 - 11:41 PM

Another note, rhizo is overrated. Just make sure it's obviously myc. No need to isolate homogenous looking growth, you're just limiting the chances of having fruitable genetics IMHO.

 

 

Is it though? I'll concede that rhizomorphic growth can be induced by limiting nutrients and that tomentose growth isn't necessarily bad by any stretch, but good rhizo often indicates that the mycelium is healthy and vigorously "looking" for nutrients, which is generally a good trait to harness IMHO.

 


 

 

 

Another note, rhizo is overrated. Just make sure it's obviously myc. No need to isolate homogenous looking growth, you're just limiting the chances of having fruitable genetics IMHO.


That’s really interesting. Did you learn this experimentally or from some source?

 

I mean, the one thing I can say about cleaning up or letting it go wild is, from research, you get more consistently grown caps rather than a jungle of stuff. It's still good, I'm sure, but you'll have a lot of different growths and stuff.

 

 

Wait what?

 

( Good idea sandman ) OP  could get fancy and ad an SHIP (self healing injection port ) or mae one with regular clear silicone no need to by RTF ....        FANCY

 

Gazeebo, I love you, full stop.

 

 

 

Another note, rhizo is overrated. Just make sure it's obviously myc. No need to isolate homogenous looking growth, you're just limiting the chances of having fruitable genetics IMHO.


That’s really interesting. Did you learn this experimentally or from some source?

 

I used to isolate and try and get the monoculture or whatever people are shooting for with that agar porn. Tested out multiple "isolates" some overlayed like crazy and never fruited. Others just side-pinned or had sparse pinning. Don't think it's my conditions. After 20 different strains and maybe a few 1,000s of plates I'm confident as others have seen with MSS syringes, the closer to a germ plate the better chance of a "canopy" or better fruits to clone from. 

Also, if you've poured enough plates at different thickness with different media you'd also know rhizo almost switches back to tomentose or whatever depending on variables I can't control for the most part. There's also a tomentose gang out there...

 

Look at Fahts work with rubbing different strains together, he's going direct from sporeload. 

 

 

With all due respect, you're wrong.

 

Firstly, being so casually dismissive of your fellow citizen scientists in their pursuit of "monoculture or whatever" indicates to me that you don't understand what you're talking about.

 

If one truly isolates out a given set of genetics, that's the only way to truly test variable growing "conditions".

 

Just because you've poured 1000's of plates, doesn't mean that you know what you're doing. For example, you said this:

 

"Also, if you've poured enough plates at different thickness with different media you'd also know rhizo almost switches back to tomentose or whatever depending on variables I can't control for the most part. There's also a tomentose gang out there..."

 

Aside from being condescending "if you've... then you'd also know", what are these variables that you can't control? The point of isolating out genetics, is to limit the variables.

 

I've poured a few plates too.

 

post-147940-0-24312200-1627447134.jpg

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#193 rhapsodyclaymore

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Posted 28 July 2021 - 06:13 AM

I picked the rest of the mushrooms I'll do on this 3rd dehydrator cycle. This includes the ones picked in an earlier post that I had stored in the refrigerator in a plastic bag with a paper towel wrapping it (changed once a day to keep it dry).

Total in this new cycle is 163.5g wet (fresh picked). That should dry down nice to around 16g dried or more.

Edited by rhapsodyclaymore, 28 July 2021 - 11:15 AM.

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#194 rhapsodyclaymore

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Posted 28 July 2021 - 11:19 AM

Next batch is in the dehydrator for the next 20 hours. 3rd flush is having a nice pin set right now so probably in another week to 12 days I'll have another full batch again. This is just a very crazy amount of mushrooms! (not complaining)



#195 rhapsodyclaymore

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Posted 29 July 2021 - 01:45 AM

I just picked another 79.8g wet today. I'll put these in the refrigerator as usual and wait for any of the other caps to start to mature. The current dehydrator cycle with the other batch will finish in the next 5 hours today.



#196 rhapsodyclaymore

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Posted 29 July 2021 - 07:24 AM

Ok, dehydrator session is now done. 16.8g dried from the batch running the last 20 hours.

 

The total now in reserve is 69.2g dried.

 

There's still 79.8g wet in the fridge to dry next, but I'll give it another day to see if any of the other mushrooms in the bin want to be ready.

 

Onward to the 3rd or 4th flush!



#197 Oldpunk

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Posted 29 July 2021 - 08:32 AM

Wow. You guessed pretty close on that dried weight. I applaud your attention to detail. I'm always in too big of a hurry to to keep track or even measure/weigh things.

If you keep getting more fruits and break 100g with one tray it should set you up good for a while. That's a lot of mushrooms.

#198 rhapsodyclaymore

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Posted 29 July 2021 - 08:38 AM

I'm only documenting here. Not writing anything down anywhere else. So this thread is literally turned into a grow journal. A good one at that. But also as a good log to show that when doing everything correct and patiently, anyone can have as good of results as what I produced.

Btw, the dry weight left is what I have left. Minus 41g wet (let's say around 4.5g dry) plus 2.6g dry from last weekend and 10g I gave away. In total, combined with what I've got fresh in the refrigerator, that's really getting close to 100g dry total. That's just amazing!

Hoping for good results on the remaining flushes.

My next project is to try and revitalize that old GT spore print I have with a syringe. After successfully doing that, I want to try and bring to life that old ~20 year mystery hybrid strain print I got from an old grower. It's good practice anyway to work with old prints because if for whatever reason I need to stop for a while, I'd like to be able to get any prints I have back in working order.

Edited by rhapsodyclaymore, 29 July 2021 - 08:57 AM.


#199 rockyfungus

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Posted 29 July 2021 - 07:46 PM

 

Another note, rhizo is overrated. Just make sure it's obviously myc. No need to isolate homogenous looking growth, you're just limiting the chances of having fruitable genetics IMHO.

 

 

Is it though? I'll concede that rhizomorphic growth can be induced by limiting nutrients and that tomentose growth isn't necessarily bad by any stretch, but good rhizo often indicates that the mycelium is healthy and vigorously "looking" for nutrients, which is generally a good trait to harness IMHO.

 


 

 

 

Another note, rhizo is overrated. Just make sure it's obviously myc. No need to isolate homogenous looking growth, you're just limiting the chances of having fruitable genetics IMHO.


That’s really interesting. Did you learn this experimentally or from some source?

 

I mean, the one thing I can say about cleaning up or letting it go wild is, from research, you get more consistently grown caps rather than a jungle of stuff. It's still good, I'm sure, but you'll have a lot of different growths and stuff.

 

 

Wait what?

 

( Good idea sandman ) OP  could get fancy and ad an SHIP (self healing injection port ) or mae one with regular clear silicone no need to by RTF ....        FANCY

 

Gazeebo, I love you, full stop.

 

 

 

Another note, rhizo is overrated. Just make sure it's obviously myc. No need to isolate homogenous looking growth, you're just limiting the chances of having fruitable genetics IMHO.


That’s really interesting. Did you learn this experimentally or from some source?

 

I used to isolate and try and get the monoculture or whatever people are shooting for with that agar porn. Tested out multiple "isolates" some overlayed like crazy and never fruited. Others just side-pinned or had sparse pinning. Don't think it's my conditions. After 20 different strains and maybe a few 1,000s of plates I'm confident as others have seen with MSS syringes, the closer to a germ plate the better chance of a "canopy" or better fruits to clone from. 

Also, if you've poured enough plates at different thickness with different media you'd also know rhizo almost switches back to tomentose or whatever depending on variables I can't control for the most part. There's also a tomentose gang out there...

 

Look at Fahts work with rubbing different strains together, he's going direct from sporeload. 

 

 

With all due respect, you're wrong.

 

Firstly, being so casually dismissive of your fellow citizen scientists in their pursuit of "monoculture or whatever" indicates to me that you don't understand what you're talking about.

 

If one truly isolates out a given set of genetics, that's the only way to truly test variable growing "conditions".

 

Just because you've poured 1000's of plates, doesn't mean that you know what you're doing. For example, you said this:

 

"Also, if you've poured enough plates at different thickness with different media you'd also know rhizo almost switches back to tomentose or whatever depending on variables I can't control for the most part. There's also a tomentose gang out there..."

 

Aside from being condescending "if you've... then you'd also know", what are these variables that you can't control? The point of isolating out genetics, is to limit the variables.

 

I've poured a few plates too.

 

Sorry to come off as condescending. I don't question your knowledge.

To keep it short and sweet. I keep my nutes and agar low. I like the way the agar handles and grows out. I don't think a uniform rhizo culture should be the end point. Insure you have clean myc and go to grain so you can print. Clone from there so you can isolate the genetics.

It's IMO a crapshoot and waste of resources to seek homogeneity. Instead the earliest you got clean myc the earliest you have a ton of different things to possibly clone or free up space.

I love rhizo as much as the next as it's easy to tell you got clean, healthy, growth. Hell i'd kill for any clean myc right now!

 

My next project is to try and revitalize that old GT spore print I have with a syringe. After successfully doing that, I want to try and bring to life that old ~20 year mystery hybrid strain print I got from an old grower. It's good practice anyway to work with old prints because if for whatever reason I need to stop for a while, I'd like to be able to get any prints I have back in working order.

 

Hydration and patience is the key to older prints. May want to make a syringe or look into agar water or low low nute agar. 

Looking good rhapsody, keep it up! I suck at keeping notes too. 


Edited by rockyfungus, 29 July 2021 - 07:47 PM.

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#200 rhapsodyclaymore

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Posted 31 July 2021 - 06:09 AM

Ok, I'm back. I just picked another bunch totaling 98g wet. I've got 98g wet I've already picked in the refrigerator from 2 days ago that was saved. Today I'll run another dehydrator cycle and wait on the next flush to happen in a few more days.

I have ~79g dry in storage right now. With the new batches totaling ~156g wet, it should dry down to about 17g. Possibly granting me 100g dry in reserve right now. So, it would appear on my first grow that I've achieved so far roughly 116.5g dry from what I have used right now.

I'll check back in again tomorrow once I have the final dry weight from the dehydrator cycle.

I've been attempting to make the side pins lesser happening by every couple days misting the top of the inside wall of the box heavily. This misting doesn't hit the substrate surface or the pins, but drips down the walls of the box into the bottom area to keep it quite wet to attempt to stop the side pins. And, it also keeps the bottom of the substrate wet so I don't have to do so much heavy soaking.

Edited by rhapsodyclaymore, 31 July 2021 - 07:22 AM.





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