I'm not sure where the best place for this question is, but I'm growing edibles...
Anyways, I watched several youtube on the Fresh Farm Fungi channel. He has a workflow where he dilutes the spores and plates them. When the individual spores germinate he transfers mycelia (ium?) to another plate. He would then let it grow out and introduce it to another mycelium to see if they could pair.
The part of this workflow that concerns me is if a sparse amount of spores germinates. Say there is only one visible white spot on a plate. Do I need to assume that is monokaryotic and try to pair it if possible or assume just assume it's sterile?