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My Spores to Agar Journey


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#1 Oam

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Posted 26 June 2021 - 12:12 PM

GT spore print (streaks of mycelium cup), B+ spore print ( circular growth pattern cup), and a gifted unknown Albino or Luecistic semi dry specimen ( four or more separate portions of growth cup). I decided to go with MS from the gills with the specimen instead of taking a tissue clone. I knew my chances for contam were high with this method, but I proceeded. Everything was done with oven tek, a flame and an inoculation loop . The agar was PC’d before being poured to cups with the oven tek as well. The mycelium is consuming the blue dye. Started on 6/11.b1326b440cf5078b5ce44db6024b6099.jpg
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#2 phlegmbae

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Posted 26 June 2021 - 02:00 PM

It looks like you're following PGT. I've wondered about that tek. So far they look pretty clean.


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#3 Oam

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Posted 28 June 2021 - 07:18 AM

Today is Lab day. I plan to do some transfers. 6ca9cd90df8a56c3d774138cb68764a4.jpg
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#4 Oam

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Posted 29 June 2021 - 01:27 PM

I had to hold off on transferring yesterday after taking a look at some of my previously made agar cups growing contams. I sorted through the rest of the clean ones today and got to transferring. I made two transfers for each of the 3 species T1-A & T1-B for now. I’ll save the remaining clean cups for Tranfer #2.
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Edited by Oam, 29 June 2021 - 01:41 PM.

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#5 Oam

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Posted 02 July 2021 - 06:04 AM

My first transfers have been running successfully without contams so far. I need to work on getting the transfer unstuck from my blade in a better manner. I’m pretty surprised by the results of the gifted specimen I transferred. 4d9a70b1de1b17791a9eca991067766e.jpg
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Edited by Oam, 02 July 2021 - 06:14 AM.

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#6 Salty117

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Posted 02 July 2021 - 09:08 AM

I wish I could get my specimen to look this good on agar. For some reason I find myself with rhizomorphic growth from the point of inoculation of my agar but it looks gelatinous.


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#7 Oam

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Posted 03 July 2021 - 05:19 AM

Morning update 37cf3edb323185ae29e880e5b55802f6.jpg
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#8 phlegmbae

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Posted 03 July 2021 - 01:19 PM

Looks like it's taking off pretty good. Kudos!


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#9 CatsAndBats

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Posted 05 July 2021 - 02:50 AM

OP, oven tek is extremely flawed in my opinion. A solid SAB, GB or a LFH will suit one's needs for aseptic agar work.


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#10 Oam

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Posted 05 July 2021 - 06:40 AM

OP, oven tek is extremely flawed in my opinion. A solid SAB, GB or a LFH will suit one's needs for aseptic agar work.

I agree that oven tek has its flaws (I sweat a lot). SAB method does work, but I just wanted to experiment with a different method. I’m currently working on my LFH build. My hepa filter is sitting in the box . I just need to snag a fan and start my build.

#11 Oam

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Posted 05 July 2021 - 06:43 AM

Morning update
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#12 Oam

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Posted 07 July 2021 - 05:37 AM

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Might do transfers today in my SAB. I’m really digging the Mickey transfer .

Edited by Oam, 07 July 2021 - 06:08 AM.

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#13 rockyfungus

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Posted 07 July 2021 - 06:48 AM

Any way to shrink those photos, they are an eye sore  :tongue:


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#14 Arathu

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Posted 07 July 2021 - 06:26 PM

The work is killer! Nice job.......keep going you will go far doing things like that....and good luck on that hood....you'll love it!

 

Photo's are huge, I shrink things to 1024 X 768 before posting them (some folks don't even like them that big, more like 800 X 600)......

 

A


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#15 Oam

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Posted 09 July 2021 - 06:33 AM

My T2-A/B went well in my SAB. I know dye is a touchy subject vs charcoal and such, but I enjoy the breakdown process to help me keep track of which transfers are older by the lack of color in the agar . I selected the small photo upload option in the app instead of original if that helps.

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Edited by Oam, 09 July 2021 - 06:36 AM.

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#16 Sidestreet

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Posted 10 July 2021 - 10:58 AM

Yeah that makes them a lot easier to look at!  Thanks.  :)

 

The only thing negative I've heard about dye is that people avoid some colors because they contain Yellow 5 or something--actually I think it was a red but I can't remember.


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#17 Oam

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Posted 11 July 2021 - 07:35 AM

Morning update
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#18 Oam

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Posted 19 July 2021 - 08:23 AM

I’ve been tending to my dog who will be taking his rainbow journey today, so my plates have been at the bottom of my list. I’m calling this next grow Mako. Love ya boy. May I see you on my next journey.76ae60145338cf2c49ac10bfd5408767.jpg
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Edited by Oam, 19 July 2021 - 11:55 AM.

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#19 Sidestreet

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Posted 19 July 2021 - 05:46 PM

So sorry to hear that Oam.  :hug:


Edited by Sidestreet, 19 July 2021 - 05:46 PM.

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#20 Arathu

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Posted 19 July 2021 - 05:53 PM

Damn! Sorry to hear that...... :hug:

 

A


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