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My Spores to Agar Journey


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#41 Oam

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Posted 19 August 2021 - 08:23 AM

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#42 MushLuvR

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Posted 20 August 2021 - 12:26 PM

I've never found a need to color up my Agar plates, BUT it sure does look COOL.   :biggrin:


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#43 Oam

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Posted 23 August 2021 - 07:35 AM

I’ll try my new agar punch method with this next set of transfers.2313450053d924f2165ca577d8547ebc.jpg

Edited by Oam, 23 August 2021 - 09:06 AM.

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#44 Oam

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Posted 24 August 2021 - 11:18 AM

The only issue I see with this agar punch method is the different depths of my agar pours. Outside of getting that fine tuned, I enjoy this method.5f4ab76b36460ecc118802c7b140142d.jpg
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#45 Oam

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Posted 27 August 2021 - 12:06 PM

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Edited by Oam, 27 August 2021 - 12:17 PM.

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#46 Oam

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Posted 29 August 2021 - 06:25 AM

Houston, we have rhizo mycelium from the experimental dried Albino/Leucistic specimen. This is my first time seeing rhizo and I’m pretty excited.
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Edited by Oam, 29 August 2021 - 06:26 AM.

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#47 Arathu

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Posted 29 August 2021 - 07:13 AM

AWESOME! Very nice work Oam......

 

IMHO....from the the first plate/cup in the first picture, I would make very small transfers from the leading edges of the rhizome growth at the 9 o-clock, 10 o-clock, and 11 o-clock positions......

 

Grow those bad boys out and once convinced they are nice and clean (transfer again if necessary) take them to sterile grain for expansion.........

 

Beautiful!

 

A


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#48 Oam

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Posted 29 August 2021 - 09:22 AM

AWESOME! Very nice work Oam......

IMHO....from the the first plate/cup in the first picture, I would make very small transfers from the leading edges of the rhizome growth at the 9 o-clock, 10 o-clock, and 11 o-clock positions......

Grow those bad boys out and once convinced they are nice and clean (transfer again if necessary) take them to sterile grain for expansion.........

Beautiful!

A

Thank you [mention]Arathu [/mention]. I value your opinion for sure. I’m enjoying the clock reference for navigating the transfers. The leading edges of 9,10, & 11 o-clock it is.
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#49 rockyfungus

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Posted 29 August 2021 - 09:32 AM

Oam looking good! I think your "rhizo plate" has some tag-along mold though. I'd transfer it till you get a bit more even. Whenever you have scalloped edges be weary about something inhibiting the mycs growth. Almost can see that second fluffier growth at 3:00 and the satellite at  5ish. 
I believe Artathu is raising the same point. 

So close!


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#50 MushLuvR

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Posted 29 August 2021 - 09:49 AM

I would agree with Rocky and Arathu, looks like possible Wet spot mold, the good news is, it's one of the easiest to clean up.  Here is a Wet Spot contamination from another dirty syringe.

 

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I shook the plate lightly to move the seeds away from each other,

and every single place the seed touched, created Wet Spots.

 

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Clean Plate of Seeds that I'm currently testing.  I did the same thing,

moved the seeds around, No extra "Spots".  


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#51 Arathu

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Posted 29 August 2021 - 10:02 AM

Agar is our playground......indeed.....

 

Ironically enough.......... "As above, so below" ...........(on SO MANY levels)

 

Sectioned plates and also multiple layers of Agar can be experimented with too.....remember that mycelium wants to grow/expand spherically not just flat.....

 

Now, this is fun.....

 

A


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#52 rockyfungus

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Posted 29 August 2021 - 12:22 PM

Also loves to switch growth patterns between nutrients/thickness/temperature?

 

I just get a little weary when it's pinched in. I don't need perfectly uniform and round. Just very homogenous. 


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#53 Oam

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Posted 29 August 2021 - 12:54 PM

Here goes nothing with 9,10, & 11 o-clock transfers on Petri dishes I saved for last. I was pressed for time, so I didn’t experiment with my punch method. Thank you for everyone’s input. Mush luv
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Edited by Oam, 29 August 2021 - 12:57 PM.

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#54 Arathu

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Posted 30 August 2021 - 06:07 PM

I ran out of likes yesterday....

 

A


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#55 Oam

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Posted 31 August 2021 - 06:56 AM

Morning update. So far we are golden like showers. I’m in lab luv with my actual tiny Petri dishes .
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#56 MushLuvR

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Posted 31 August 2021 - 12:26 PM

You made me laugh out loud with the Golden Showers Reference... OR you didn't mean it that way with the word play... Funny all the same.  


Edited by MushLuvR, 31 August 2021 - 12:27 PM.

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#57 Oam

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Posted 31 August 2021 - 12:54 PM

You made me laugh out loud with the Golden Showers Reference... OR you didn't mean it that way with the word play... Funny all the same.

You’re welcome.
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#58 Oam

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Posted 01 September 2021 - 05:16 AM

The 9 o-clock transfer got a visit from Tammy unfortunately. This was due to an error on my part with the transfer. I’m tempted to clean it up. The other two are running well.434a66e52c416c6a5b66850ce6266f1e.jpg
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#59 rockyfungus

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Posted 02 September 2021 - 07:08 AM

Is Tammy, Trish’s spunky sister?
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#60 Oam

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Posted 02 September 2021 - 07:55 AM

10&11 are looking good. Can anyone guess what I’m building ?4e792fc3c5b7bec5fd3425f8c54f77e8.jpg
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