Ooooo CLONING .................do you have a clean air box ...eeeerrr
i mean( still. air. box ...SAB )to work in ?or a Laminar ? That will help !
A Lot !
So i can suggest a cpl ways to clone
You can Clone a cpl ways and doing multiple jars or plates ....
more chance of success...
You can clone ether on agar/a plate /or no poor agar jar or whats called a small grain proof jar .
a grain proof jar is easy you can use them to test spores also to see if there clean . some clones do better on agar some better with grain proof so do both ..if you like .
OK to make small grain proof jar get a few 4 oz clear side canning jars/not the diamond quit ones ..OK A BUNCH ha
i buy the generic clear side 4 oz jars on amazon
OK modify the lid put a filter patch over a vent hole or plug with pollyfill for (FA ...fresh. air. )
Also on the lid make a (SHIP self .healing. injection. port )with some silicone... regular clear silicone is ok/best
you don't need high temp RTV silicone .regular clear silicone actually can be PCed and regulhigh clear silicone stays more flexible then the more expensive i temp RTV .
ok fill your 4 oz jars ...about half full of your prepped grain , PC/sterilize the modifyed 40 oz filled small grain proof jar and or make your sterile agar jars or plates ....the clear 4 oz clear side jars make a good no poor agar jar .
Now take a clean the mushroom to be cloned with alcohol take stem tear down the middle from the bottom in you SAB cut out small sample of sterile flesh from center inside of stem and put in sterile grain . do several if one or two grow clean/colonized ....
NOW you can inject clean/sterile water into small grain proof jar with LARGE syringe ...SHAKE then fill regular 10 cc sterile syringes with mycelium water to use to inoculate your spawn or cakes ect . they will take off way faster then inoculating with spores... you can select the best clusters or biggest mushrooms ect improving your culture .
or as mentioned above you can use the flesh from center of your stem to put tiny peace on agar ...so if a agar plate or no poor agar jar grows clean you can use wedges of the mycelium agar to inoculate ..
ok i hope i haven't confused you .
good luck cloning
Edited by bezevo, 15 July 2021 - 11:10 AM.