Loveall and Shroombee's work
Unfortunately due to operator error during the CIELO, the final results are not likely true.
It started with them harvesting 5 columns of cacti, maybe 2.5"x18"ea? Slice away most of the white inner flesh. They then cubed them, tossed them in the dehydrator and ground them in a blender. The result, 174g powdered cacti to be split between the two experiments.
They started w/the A/B putting white vinegar and 87g cacti powder in the crock pot for 6 hrs x3 and water 3hrsx1. After cooling a bit, added the pectic enzyme and let sit. They drained it through a dish towel, squeezing to collect the liquid then they ran it through a funnel stuffed with a couple cotton balls. The cleaned liquid was boiled down to a quart and Greenskeeper's recipe was followed for the rest of the way. Unfortunately, during salting, the first was too acidic, 2nd was much better, 3+ was right on. They pulled 1.29g sticky residue before washing and 1.22g after the wash. Might need 1 more wash?
The CIELO procedure, the # were adjusted for 87g powder instead of 100g. The milky water was made and the cacti powder was mixed in. Before microwaving, it has a texture of mousse. After microwaving it was dryish and sandy. Unfortunately, this is where the error occurred (I believe?). If you look at the picture of the glass bowl after the microwave, you'll see a residue stuck to the side (pic 1). I think they should have done a better job scraping the side of the bowl in between microwave bursts. By the end of the saponification, the residue was stuck, scraping could not remove it so they tried to wash it with EA, no luck. Later, they said hot water took it off. If they'd been thinking, they would have collected the water and rerun it in a mini extraction.
Moving forward w/the CIELO, they had to decant the EA off of the paste so they added a couple extra pulls in there. All the pulls were done w/freezer cold EA except the last which was done w/warm. To make sure the EA extract was clean, after running through a coffee filter, they ran the combined pulls through a glass turkey baster stuffed w/a couple cotton balls. Because of the extra pulls, they had more than a quart and used a 1/2 gallon mason jar to salt in. To be sure, they added 20g citric acid and swirled hard for about 3min. The jar was then placed in a quiet spot for about 20 hours. You could see a residue had formed but was stuck to the walls (pic 2). The salted EA was poured through a glass turkey baster w/cotton balls. The excess EA was blown out of the baster and the contents allowed to dry for a few minutes. Then warm water was poured into the mason jar and swirled for a minute then through the baster, collected and evaporated. Due to error, the end result, only 0.1g of salts. The salt tasted slightly salty, slightly bitter with a little sour in the finish.
Comparing the two procedures, CIELO is much safer, much faster and easier! Your whole extraction, up to evap, could be done in the time your are completing the acid phase of the A/B. I'm disappointed there cannot be a direct comparison of the yields do to operator error! Hopefully they'll do a proper comparison in the future. I think the next test will be using CIELO with freshly harvested columns.
The end products are beautiful! The citrate is a rose colored very fine crystal (pic 3). The HCl a lovely orangish, slightly tacky crystalline product (pic 4). Hopefully some experimentation......on lab rats :) ....... is in order!
Edited by YoshiTrainer, 17 July 2021 - 10:59 AM.