First off thanks to all who have shared info on this site. Itd be rough figuring it out through trial and error. Your knowledge is greatly appreciated.
One thing ive been trying to figure out is during incubation, do you want any air exchange through your filter ports on the jar lids. Or is the primary function of whichever filter material(tyvek,poly,micropore) to allow pressure to escape during sterilization. I know ive seen teks somewhere that show inoculation holes being covered with masking tape which seems like itd stop air exchange. Also, I thought ive read where mycelium thrive in co2 rich environments and only require fresh air when fruiting begins.
That being said i know stale air supports the growth of unwanted organisms. Also, Ive noticed after a G2G mycelium looks incredibly healthy and colonizes the surrounding nutrient source very rapidly in the following days.It appears much healthier and I can only guess thats from the fresh air introduced by opening the jar.
I might be mistaken and completely off with my recollection so apologies in advance if thats the case. Just trying to gain a solid understanding of the entire process. I dont like to "think" i know something.