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Instant Pot - Laboratory Grade Sterilization Supported by Research

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#1 simplegood78



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Posted 28 October 2021 - 01:32 PM

I wish I could claim the research was mine (not really), but it belongs to Vaille Swenson.  She is currently a Virology and Gene Therapy PhD student at the Mayo Clinic.




In 2019, she wrote her Masters Thesis titled "Assessment and verification of commercially available pressure cookers for laboratory sterilization"

She looked at pressure cookers by Cosori, Gourmia, GoWISE, and Instant Pot.


The following table summarized the time needs to sterilize agar and soil water.




The Instant Pot passed the spore sterilization test, even against known heat resistant Geobacillus stearothermophilus spores.



A sterilization time of at least 150 mins was required for the Instant Pot to inactivate the spores

(Figure 2), which appeared similar in color and opacity to a spore ampoule sterilized in an
autoclave and the negative control. None of the other pressure cookers inactivated the spores
even with sterilization times of up to 300 minutes, which yielded unsterilized spore ampoules
visually identical to a positive control. These data indicated that the Instant Pot pressure cooker
could sterilize spores to the level of an autoclave, and we infer that it would be capable of
inactivating all other less heat resistant organisms to properly sterilize laboratory items for


It took 150 minutes to eliminate that spore.  So I would imagine bags of spawn would require at least 150 minutes, if not longer.


The complete thesis can be found --> here for interested parties.




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#2 rockyfungus



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Posted 28 October 2021 - 03:54 PM

Believe mine only goes up to 120. They work. Done it all in it. Just cramped and the 120 is limiting. When I first started I would double run it for bags 240 mins. 

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#3 dead head jed

dead head jed

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Posted 31 October 2021 - 08:52 PM

I do grain jars in mine and love that its set and forget. Have left jars sitting on the table for 2 weeks and stay nice snd clean. Unfortunately its when I open them to add cultures for 1.5 sec that they all seems to start growing green.
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