Paradox
©
Fisana

Jump to content


Photo
* * * * * 2 votes

Golden Reishi cloning


  • Please log in to reply
18 replies to this topic

#1 pr0wlunwoof

pr0wlunwoof

    Mycophage

  • Free Member
  • 106 posts

Posted 03 November 2021 - 01:54 PM

Recently got into Mycology and I have been on the hunt for a wild edible species. Well I found a Golden Reishi on a walk with the family. Decided to clone it and grow it out for medicinal use. So far I have made it to transfer 1 on Agar. 

 

Reishi Mushroom.jpg


Edited by pr0wlunwoof, 03 November 2021 - 01:55 PM.

  • Arathu, YoshiTrainer and MushLuvR like this

#2 pr0wlunwoof

pr0wlunwoof

    Mycophage

  • Free Member
  • 106 posts

Posted 03 November 2021 - 01:59 PM

Here is the plate before I did the transfer. 

Attached Thumbnails

  • Reishi on Agar.PNG

  • Arathu, rockyfungus and MushLuvR like this

#3 rockyfungus

rockyfungus

    Mycotopiate

  • Free Member
  • 2,347 posts

Awards Bar:

Posted 03 November 2021 - 04:00 PM

Looks good away from that slime mold, of course


  • MushLuvR likes this

#4 pr0wlunwoof

pr0wlunwoof

    Mycophage

  • Free Member
  • 106 posts

Posted 03 November 2021 - 06:27 PM

So is the non white part slime mold? One of the 4 original has only that on it.b0f4eb43c6da3c109538cdaf9be6f1e8.jpg

Sent from my SM-N986U using Tapatalk

#5 pr0wlunwoof

pr0wlunwoof

    Mycophage

  • Free Member
  • 106 posts

Posted 03 November 2021 - 06:31 PM

Looks good away from that slime mold, of course

I just looked up slime mold. I couldnt figure out what that was. Thanks for the help. All but one original had that visible. 6debedc46e496b221d0c78282c543c67.jpg

Sent from my SM-N986U using Tapatalk

#6 MushLuvR

MushLuvR

    Mycotopiate

  • Gold VIP
  • 951 posts

Awards Bar:

Posted 04 November 2021 - 11:54 AM

You have plenty of clean culture to work with... Very Nice!


  • rockyfungus likes this

#7 pr0wlunwoof

pr0wlunwoof

    Mycophage

  • Free Member
  • 106 posts

Posted 05 November 2021 - 07:22 AM

Transfers are looking good. The only original one without slime mold is looking good as well.2d2e5caf35aa0eb76153ee1b5b91eee8.jpg

Sent from my SM-N986U using Tapatalk
  • MushLuvR likes this

#8 pr0wlunwoof

pr0wlunwoof

    Mycophage

  • Free Member
  • 106 posts

Posted 09 November 2021 - 08:30 AM

Update on my plates. Might have to move to grain this weekend.6176b4d52515fb5fd86c3a2c5fd63c61.jpg

Sent from my SM-N986U using Tapatalk
  • MushLuvR likes this

#9 Arathu

Arathu

    Dirtmaker

  • OG VIP
  • 7,430 posts

Awards Bar:

Posted 09 November 2021 - 07:05 PM

Nice work! Keep going for sure, we found a golden Maitake last year, cloned it, and it has an extremely persistent bacterial component to it....

 

I'd make at least another set of plates and transfers before jumping to grain and do a tester before committing to a big grain run.....those are looking good but still could use a bit of cleaning IMHO

 

Sweet capture! I hope that fruits for sure....be something to see....

 

A


  • Needles and MushLuvR like this

#10 pr0wlunwoof

pr0wlunwoof

    Mycophage

  • Free Member
  • 106 posts

Posted 10 November 2021 - 10:39 AM

Thanks for the input. The two bottom are transfers the top left is an original. I might do a single jar with the bottom left and do another couple transfers of the bottom right before going too big. 

 

Since this is my first agar experience and my first clone. What am I looking for outside of fluffy white mycelium?

 

Nice work! Keep going for sure, we found a golden Maitake last year, cloned it, and it has an extremely persistent bacterial component to it....

 

I'd make at least another set of plates and transfers before jumping to grain and do a tester before committing to a big grain run.....those are looking good but still could use a bit of cleaning IMHO

 

Sweet capture! I hope that fruits for sure....be something to see....

 

A

 



#11 rockyfungus

rockyfungus

    Mycotopiate

  • Free Member
  • 2,347 posts

Awards Bar:

Posted 10 November 2021 - 09:29 PM

We are looking for homogenous round plates typically. You’ll eventually learn the nuances of each myc.

See how some have notches. That most likely indicates something there the myc is avoiding. You may luck out cutting a few squares away from there and transferring to grain.

Easier to go out another plate and if it looks more symmetrical, SEND It!
  • MushLuvR likes this

#12 pr0wlunwoof

pr0wlunwoof

    Mycophage

  • Free Member
  • 106 posts

Posted 11 November 2021 - 08:25 AM

Thanks for the clarification. I see how the semetry makes sense now.

Sent from my SM-N986U using Tapatalk

#13 Needles

Needles

    Locked and Loaded

  • OG VIP
  • 1,395 posts

Donator


Awards Bar:

Posted 16 November 2021 - 04:46 AM

The plate with the ship looks the best. The plate you posted a pix of earlier looks like a type of bacteria infection rather than mold. Bacteria can hitch hike on mycelium as a symbiotic relationship. Arathu mentioned a wild strain we have cloned, testing results concluded that its harboring a gram positive bacillus. We’re on transfer 8 now with it and still no monoculture. For just getting started you have done a fabulous job with your clone. Looking forward to seeing you move it to grain…….


  • Arathu and MushLuvR like this

#14 rockyfungus

rockyfungus

    Mycotopiate

  • Free Member
  • 2,347 posts

Awards Bar:

Posted 16 November 2021 - 12:34 PM

That yellow growth is not a slime mold? Or are you talking about the scalloped plates?



#15 pr0wlunwoof

pr0wlunwoof

    Mycophage

  • Free Member
  • 106 posts

Posted 26 November 2021 - 09:57 AM

Well. I did not do an additional tranfer as I made a batch of spawn and decided to give it a whirl. The beginners luck appears to be continuing.

What do you guys think? Ready for bulk?

I still have 2 agar plates to mess with if need be.3e1bf01f86af0ac5ec9849f2e4b3b9ab.jpg076b921b941820a68ef0270783a3aea1.jpgd5d5a1d9ed84a6aedf7068339bd044f2.jpg

Sent from my SM-N986U using Tapatalk
  • MushLuvR likes this

#16 pr0wlunwoof

pr0wlunwoof

    Mycophage

  • Free Member
  • 106 posts

Posted 30 November 2021 - 08:13 AM

I think we can count the transfer as a success.0196f79391c6dc0f8c4d63c2517a5663.jpg675a38dd0323e023f226d9af181d44b3.jpg

Sent from my SM-N986U using Tapatalk

Edited by pr0wlunwoof, 30 November 2021 - 12:49 PM.

  • Arathu, rockyfungus and MushLuvR like this

#17 pr0wlunwoof

pr0wlunwoof

    Mycophage

  • Free Member
  • 106 posts

Posted 02 December 2021 - 08:04 PM

So the transfer to LC and another plate are coming along well. I still have some technique honing, but the laminar flow hood is proving to be sterile at this point.3e66b2915b87be7b480cbf446646294c.jpg8189778a56f5cb6d465723a6db77d2f3.jpg

Sent from my SM-N986U using Tapatalk
  • Arathu likes this

#18 Arathu

Arathu

    Dirtmaker

  • OG VIP
  • 7,430 posts

Awards Bar:

Posted 03 December 2021 - 09:53 AM

Getting a hood set-up changes things for sure.....It opens up all kinds of horizons particularly by the fundamental confidence it provides and directly proves.....

 

I like to sit in the stream from my hood.......I even get sinus relief from it......

 

You'll quite likely amaze yourself. That's very nice work, I want to see that fruit as golden.....

 

Man the possibilities are endless.....

 

A



#19 pr0wlunwoof

pr0wlunwoof

    Mycophage

  • Free Member
  • 106 posts

Posted 03 December 2021 - 02:00 PM

I shook the two grain jars I did on 11/14 last night. Hopefully I get a moment to pasteurize some pellets this weekend and we can get everything on bulk. 






Like Mycotopia? Become a member today!