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#21 Salty117

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Posted 08 November 2021 - 09:27 AM

Trich = Trichoderma, which is a very aggressive and hard to kill fungus, and it's also very common. Trich is often found in the dirt so manure has a tendency to pick up on the spores that are ever-present in our environments

 

And yes by transfer I mean cutting a tissue sample out from the agar plate and stick it on another agar plate by itself


Edited by Salty117, 08 November 2021 - 09:29 AM.


#22 xXHeathenXx

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Posted 08 November 2021 - 09:44 AM

I'm not too sure about what exactly is going on in the Burma plate, but the PE plate looks like it could have some good mycelium cultures starting. I just got my agar journey started this year so I'm still learning myself, but I would think that getting those cultures transferred before they lay over each other should help isolating in fewer transfers.

The Burma agar was on a tilt in my inoculation box so all the spores went to one side

#23 rockyfungus

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Posted 08 November 2021 - 09:55 AM

Trich is the green contaminant we encounter often. Hard to tell but that's what it looks like on my plates. Within a day or two it'll turn green if it's bad.

trich.jpg

Transferring is moving a piece of agar (that's desirable) to a new plate of agar. Basically if anything looks diff then what you put on the plate you want to stay away from the bad and grab a clean piece to move. Scalpel, needle, leather punch, etc. Anything that can be flamed to clean. 

In my pic see how there's perfectly round and darkly centered colonies. That's trich or bad.
The fluffier white is pan cyan myc (looks way diff then cubes)

 

 


Edited by rockyfungus, 08 November 2021 - 10:01 AM.

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#24 Salty117

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Posted 08 November 2021 - 10:07 AM

Using an inoculation loop to smear the spore solution can help spread the spores from spore solution around the plate. This can aid against pooling if your plate gets tilted. Maybe not fix it entirely but it should help keeping everything from spawning at one side.

 

Maybe it's my eyes, maybe it's the picture quality, but it looks like the Burma plate could have some other thing going on. The cultures on the PE plate appear to have a dense center that thins out at the edges, this is what normal mycelial growth should look like in it's early stages. Contamination will more often appear blotchy. As you work with cultures it will become easier to identify when something is clean, or if you have an unwanted guest.



#25 xXHeathenXx

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Posted 08 November 2021 - 11:20 AM

Trich is the green contaminant we encounter often. Hard to tell but that's what it looks like on my plates. Within a day or two it'll turn green if it's bad.
attachicon.giftrich.jpg
Transferring is moving a piece of agar (that's desirable) to a new plate of agar. Basically if anything looks diff then what you put on the plate you want to stay away from the bad and grab a clean piece to move. Scalpel, needle, leather punch, etc. Anything that can be flamed to clean.

In my pic see how there's perfectly round and darkly centered colonies. That's trich or bad.
The fluffier white is pan cyan myc (looks way diff then cubes)

I setup my sab w the UV light on and lysol'd & iso'd the crap out of Everything so that I can take these pics I hope it would be a cleaner look. I pulled the agar plates from the cardboard incubator w/ heat mat to control temp.
I see mycelium growth in the pe and Burma which is what these pics are. It's been since Oct 24 @ 3pm. I also have grow bags for each. Anyone know if this is mycelium that I can take a chunk of the agar and make an LC. It looks like mycelium but I'm new and would like an opinion of others that have more experience.
Thank you
20211101_143058.jpg 20211108_104556.jpg 20211108_104601.jpg 20211108_104622.jpg 20211108_104651.jpg 20211108_104706.jpg 20211108_104721.jpg

#26 xXHeathenXx

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Posted 08 November 2021 - 11:23 AM

Took the lids off to get a better look. Whatcha tink?20211101_143058.jpg 20211108_104601.jpg 20211108_104549.jpg 20211108_104622.jpg 20211108_104651.jpg 20211108_104721.jpg

#27 xXHeathenXx

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Posted 08 November 2021 - 12:12 PM

Anyone know? Is there a feed that I'm supposed to put these in for others to look? I didn't find a topic concerning this matter. I really think it's mycelium. The PE I remember getting several drops on the agar so the pe pics make me feel like that's where I inoculated the spores. The Burma also looks right considering when I placed it into the incubator box, I was tilted so all the spore solution would have all run to the same point mostly. It makes sense

#28 xXHeathenXx

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Posted 08 November 2021 - 12:13 PM

More pics from inside my sab20211108_114133.jpg 20211108_114138.jpg 20211108_114142.jpg

#29 Salty117

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Posted 08 November 2021 - 02:57 PM

The Burma looks like it's ready for transfers. I don't think that it's contamination but perhaps a more well-trained eye with more experience working with agar will chime in.



#30 Oldpunk

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Posted 08 November 2021 - 07:23 PM

If you want more opinions you could start a new thread about agar. Now it's under the heading Manure.

In pic #5 of the Burma I'd take a transfer from the top where it's starting to look stringy and probably one from the middle of the large mass. Let em grow for a week on a new plate and your good to go.
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#31 xXHeathenXx

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Posted 09 November 2021 - 08:58 AM

If you want more opinions you could start a new thread about agar. Now it's under the heading Manure.

In pic #5 of the Burma I'd take a transfer from the top where it's starting to look stringy and probably one from the middle of the large mass. Let em grow for a week on a new plate and your good to go.

Sweeeeeet. Thanks brudda, I appreciate it




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