If I need another one then I can order a pack for 8 bucks. I have nearly Everything one could need to grow gourmetDon't forget the stir bars!


Posted 08 November 2021 - 04:49 PM
If I need another one then I can order a pack for 8 bucks. I have nearly Everything one could need to grow gourmetDon't forget the stir bars!
Posted 08 November 2021 - 06:06 PM
Hey, I dropped some pics below. 1) Will that lme work?Ya I was looking into the elme extra light malt extract. But I don't reckon that would work for making an LC. Just trying to experiment and as always try to make an easier, more efficient method.Right. No agar powder. You would have lumpy cottage cheese LC.
But you can use the malt extract instead of corn syrup. That would work.
I posted pictures of the extra light malt extract recipe I use.. actively in the process of colonizing... admittedly it helps having a magnetic stir plate/bar to mix it up because it has a tendency to clump up but I just get my water kettle screamin' hot before I pour it in with my dry ingredients and it dissolves very quickly. The syrup will probably mix easier without a magnetic stir plate/bar.
Posted 08 November 2021 - 06:12 PM
That LME should work just as good as ELME. I could be wrong about that Myco+.. but I think that's for helping plant root growth? Mycelium doesn't have roots, it has Hypha/Hyphae which are the filaments of mycelium that branch out in search for food.
Posted 08 November 2021 - 07:05 PM
Ok. Is there something better than that lme? Do you have a suggestion? I'll get it from Amazonian right awayThat LME should work just as good as ELME. I could be wrong about that Myco+.. but I think that's for helping plant root growth? Mycelium doesn't have roots, it has Hypha/Hyphae which are the filaments of mycelium that branch out in search for food.
Posted 08 November 2021 - 07:18 PM
Posted 08 November 2021 - 07:20 PM
If there's any merit to the claim that grain-based sugars reduce the recovery period between inoculating grains and visible growth then I would consider Malt Extract/Light Malt Extract/Extra Light Malt Extract to be the superior sugars to use in a liquid culture. Not trying to say that corn syrup or karo syrup or honey doesn't work or isn't effective because those options will work as well but in my opinion it just makes sense to go with something that has a potentially added benefit of reducing colonization time by a few days.
Also malt extract/LME/ELME will tinge the color of the LC a slight beige. If you like the "clean and clear" look like OldPunk then corn/karo syrup are going to be the way you'll want to go.
Posted 08 November 2021 - 07:48 PM
I'm trying to find black agar mix on Amazon and I'm not having any luckIf there's any merit to the claim that grain-based sugars reduce the recovery period between inoculating grains and visible growth then I would consider Malt Extract/Light Malt Extract/Extra Light Malt Extract to be the superior sugars to use in a liquid culture. Not trying to say that corn syrup or karo syrup or honey doesn't work or isn't effective because those options will work as well but in my opinion it just makes sense to go with something that has a potentially added benefit of reducing colonization time by a few days.
Also malt extract/LME/ELME will tinge the color of the LC a slight beige. If you like the "clean and clear" look like OldPunk then corn/karo syrup are going to be the way you'll want to go.
Posted 08 November 2021 - 08:23 PM
"Black Agar" can be attained by addiing Activated Charcoal to the mix. I got a 2lb bag of it on the 'Zon like 5 months ago and I've barely made a dent in it haha. 1g of Activated Charcoal to an agar recipe calling for 500ml of water is more than adequate. I have been thinking of cutting that back to a half gram of activated charcoal because it seems to make a sort of foam on the surface of the agar mixture that can be a pain in the butt to settle.. but it is otherwise a very good additive.
Posted 09 November 2021 - 08:52 AM
I wound up finding the right agar. I think.... lol"Black Agar" can be attained by addiing Activated Charcoal to the mix. I got a 2lb bag of it on the 'Zon like 5 months ago and I've barely made a dent in it haha. 1g of Activated Charcoal to an agar recipe calling for 500ml of water is more than adequate. I have been thinking of cutting that back to a half gram of activated charcoal because it seems to make a sort of foam on the surface of the agar mixture that can be a pain in the butt to settle.. but it is otherwise a very good additive.
Posted 09 November 2021 - 09:06 AM
What's GLC?You could also look into GLC which works nicely and liquid agar is another great trick. With LA, you take clean, colonized agar, inject sterile water, use the needle tip to scrape the mycelium from the agar and suck the solution up. What is nice about this is you can be absolutely sure your mycelium is clean.
Good luck!
Posted 09 November 2021 - 09:31 AM
That "Extra Light Malt Extract" will work quite will in an agar or liquid culture recipe. But it's not agar by itself.
It's starting to seem like you're missing a few things.
Perhaps it would help if you stop and focus on one subject. The title of the thread has to do with making a liquid culture. Maybe focus on that one subject so we can try to help.
From what I'm reading from the suggestions and your replies - it seems you're not quite getting the difference between agar and liquid culture. It also seems like you're skipping over the information provided in the replies based upon your continued confusion.
The Mushroom Cultivator by Paul Stamets is well worth your time and money. An excellent book that goes a long way toward explaining myco-culture and laboratory technique.
Edited by Myc, 09 November 2021 - 09:40 AM.
Posted 09 November 2021 - 10:26 AM
Myco+ is for mycorrhizae. Or if you garden, it introduces soil fungus that can help plants grow healthier.
All you need is one nutrient source and any source of agar. Agar is a seaweed based gelatin. It's fucking jello. Jello is not nutritious so we add malt or grain water, or syrup/honey (most don't like straight sugar).
If you can't grasp this. PF TEK...
Posted 09 November 2021 - 11:10 AM
I didn't buy that myco +. I got elme and malt agarMyco+ is for mycorrhizae. Or if you garden, it introduces soil fungus that can help plants grow healthier.
All you need is one nutrient source and any source of agar. Agar is a seaweed based gelatin. It's fucking jello. Jello is not nutritious so we add malt or grain water, or syrup/honey (most don't like straight sugar).
If you can't grasp this. PF TEK...
Posted 09 November 2021 - 12:45 PM
Posted 09 November 2021 - 02:54 PM
Edited by MushLuvR, 09 November 2021 - 02:54 PM.
Posted 09 November 2021 - 04:06 PM
Hey don't PF teck shame me
Rice to bulk sub, that's all the skills I got
Posted 09 November 2021 - 05:56 PM
No pf tek shame, it's one of our most valuable tools IMHO. All you need is one cheap grain and coir. Agar to make anything with that grain water...
Edited by rockyfungus, 09 November 2021 - 05:56 PM.
Posted 09 November 2021 - 08:20 PM
Lemme ask a stupid question. C'mon , I'm new hereHey don't PF teck shame me
Rice to bulk sub, that's all the skills I got
Posted 09 November 2021 - 09:35 PM
BRF lol.
Psilocybe Fananticus Tek
Edit: Someone please insert the link to Magic Of the Masses here.
Edited by coorsmikey, 09 November 2021 - 09:40 PM.
Posted 10 November 2021 - 03:01 PM
So a cpl people have tried to enplane the difference between LC and agar plates .?.....................
Do you now understand they are two different things ? ......
For two different mycology activity's? ..
Or are you still confused that they are different variations of same thing ?
They Are Not
LC is a LIQUID CULTURE is clean inoculated water with added nutrients That you use to inoculate grain with etc
Agar is LIKE JELLO with nutrients added you grow mycelium on .to isolate and clean the mycelium culture . so buy transferring a clean /wedge of mycelium growing on the agar ... to another plate several times tell you produce a agar plate free of mold or bacteria . then you can use a bit of this cleaned up agar to directly inoculate grain or a small wedge to inoculate your LC with to expand out and use the LC to inoculate your grain jars . OK have i confused you or does that make since ?
.
Because you really seem to need to figure out and understand the difference .. .
NOW BACK TO BASICS if i were you ........i would take a Big Step Back ..........just for awhile ......and do the best beginner tek ...PF tek or BRF cakes .
Definitions... PF stands for Professor Phanaticus .. a guy who figured out a simple almost FOOL proof simple beginner tek . PF Tek
using BRF cakes..........BRF = Brown Rice Flower cakes ....
Now if you follow the link to get YOUR ...FREE ...PDF Down Load Book
READ IT a Few Times .........AND this is Important .....
Resist ...Your Mad Scientist Urges FOLLOW IT TO A "T" No substitutions ..no experiments . just follow tek exactly .
You will have simple success .
Now go to the Magic For the masses thread . and on the First Post is a Link for a FREE PDF Book on PF Tek
.i will post link below Same one mikey posted .
Again Follow it To a "T"
After a few PF BRF cake success's Then you gradually expand out to more advanced . stuff
the PF experiences will teach you a lot that will help you understand the more advance stuff you desire . BEZ
https://mycotopia.ne...for-the-masses/
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