11 replies to this topic

[danielfly]

I was super frustrated and annoyed yesterday when I tried to inoculate my pf tek jars.  In fact, I just gave up after shooting 5 cc of spores accidentally in one of the jars.  

 

I want to try again but am feeling very discouraged right now - it is very hard sometimes to push down on the syringe plunger just a little bit so only 0.25 to 0.50 cc of liquid comes out.  I try very lightly pushing but then my finger gets worn out and its nearly impossible.  Then I end up pushing harder and it squirts out almost half of my full liquid in the syringe.  

 

I use a 16 gauge 1.5 inch needle.  I'm not sure if anyone else has this problem?  And what's causing it or creative solutions to push down on the syringe plunger lightly to get a little liquid out?  

Edited by danielfly, 08 November 2021 - 01:18 PM.

[wharfrat]

 

I was super frustrated and annoyed yesterday when I tried to inoculate my pf tek jars.  In fact, I just gave up after shooting 5 cc of spores accidentally in one of the jars.  

 

I want to try again but am feeling very discouraged right now - it is very hard sometimes to push down on the syringe plunger just a little bit so only 0.25 to 0.50 cc of liquid comes out.  I try very lightly pushing but then my finger gets worn out and its nearly impossible.  Then I end up pushing harder and it squirts out almost half of my full liquid in the syringe.  

 

I use a 16 gauge 1.5 inch needle.  I'm not sure if anyone else has this problem?  And what's causing it or creative solutions to push down on the syringe plunger lightly to get a little liquid out?  

 

I have had that happen with PC'd syringes.. have you PC'd them? are they used? unless the needle is plugged, 16g should be just fine. my guess is it's a bad

syringe. Swollen rubber etc..

Edited by wharfrat, 08 November 2021 - 01:32 PM.

[surfer8210]

That’s a pretty large bore for it to be clogged.
Do you have an empty syringe?
If so, take a cup of boiling water and using the syringe and needle, pull from the cup of water a bunch of times until it clears enough for you to dose in small amounts.
You might have to do this frequently when inoculating or maybe never again.
Hopefully that all you need to do to rectify your situation.
It sounds like it’s just clogged.
Try to get it to act like a super soaker with the boiling water though.
Try alcohol also, if the water doesn’t work and then go back to the water after the alcohol.


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[danielfly]

What do you think I can do if it is a bad syringe with swollen rubber?  

[surfer8210]

Transfer the contents to a new syringe.


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[danielfly]

That's a good idea to transfer the contents to a new syringe - but won't that cause maybe some contamination getting through to the new syringe if I'm transferring it?

[surfer8210]

If you do it quickly enough you should be fine.
It’s a sterile environment inside the syringe and if you open it briefly I’m not so sure that you would worry about contamination.
It’s not like you have many alternatives.


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[danielfly]

Got it.  Its worth a try for me.  Thanks!

[xXHeathenXx]

I was super frustrated and annoyed yesterday when I tried to inoculate my pf tek jars. In fact, I just gave up after shooting 5 cc of spores accidentally in one of the jars.

I want to try again but am feeling very discouraged right now - it is very hard sometimes to push down on the syringe plunger just a little bit so only 0.25 to 0.50 cc of liquid comes out. I try very lightly pushing but then my finger gets worn out and its nearly impossible. Then I end up pushing harder and it squirts out almost half of my full liquid in the syringe.

I use a 16 gauge 1.5 inch needle. I'm not sure if anyone else has this problem? And what's causing it or creative solutions to push down on the syringe plunger lightly to get a little liquid out?

I've taken the plunger completely out and lubed the rubber with cooking oil before and it worked great. Real easy slide and responsive to light pressure

[Micogi]

Could maybe also put the syringe between your two middle fingers and the plunger against your palm. Provided your right handed, then kind of cup your left hand behind to add additional control/pressure.

I've done this using thicker glues in a syringe and worked well for me.

Being their pretty much one time use, and if you're using it all up, give it a shot.

[Oldpunk]

I've found that the clumped up spores in syringes clog the end sometimes when pointed down into jars. I tip them needle up and pull a tiny air bubble in to dislodge the blockage. All the clumped spores drop and it works good for a few more jars.

I've seen those clumps in almost All the vendor syringes. Not sure if they'd been sitting a while or what causes the clumping

[danielfly]

I like everyone's suggestions!  Will give it a try for sure!