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Laminar Flow Cabinet Build


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#21 pr0wlunwoof

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Posted 21 November 2021 - 03:12 PM

Wood working is like most things. If you have the right setup and the right tools its a whole different experience.

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#22 pr0wlunwoof

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Posted 22 November 2021 - 11:19 AM

Got first coat of polyurethane on it. Will do at least 1 more prolly 2.6e96f6edc15399643a713f8e1525be2d.jpg1f20188e686e40f6db354fb31d98c049.jpgbd4cb8c259674f6bb06ac911ec2178b2.jpg

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#23 pr0wlunwoof

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Posted 26 November 2021 - 12:53 PM

Well Turkey Day has come and gone. Here was my trade for the cabinetry work.

24 Hour Brine and a 4 hour smoke at 300f using pecan wood on the custom built gravity feed smoker. 662f52e622265a5453b5e86308cf49da.jpg

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#24 pr0wlunwoof

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Posted 26 November 2021 - 12:58 PM

Now back work on the cabinet. Finished the polyurethane and just put silicone calk on all the corners to seal it all up. All thats left are gaskets and electrical. Might actually get it done this weekend.

If anyone uses the motor I am you will need this switch to operate it at the two different speeds. It was not easy to figure out and all my electrical buddies were about useless. It added $15 to the build, but i think it will prove useful to have both speeds available.

Air Vent 58030 2SPD Wall Switch, 1-(Pack), White https://www.amazon.c...ding=UTF8&psc=1794da5be16109e79699738c63b7852f1.jpg32894b70901914ab36a1f890273b101d.jpg

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#25 pr0wlunwoof

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Posted 27 November 2021 - 10:59 AM

Got the wiring figured out. Siliconed the wire pass thru and then gotta make my permanent cord.

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#26 Myc

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Posted 27 November 2021 - 11:08 AM

Nice find on the specialized switch - DPST.

I was going to advise you to go the down and dirty route using two switches. Feed a single-pole switch with power. Run the switch leg from the single-pole to the "common" terminal of a 3-way switch. The remaining poles of the 3-way switch would be wired to your fan speeds - high and low.

Your find was much more elegant and didn't cost much more than what I am suggesting.

 

You are going to be so happy with your new tool. 

I sometimes use mine for food packaging. You can buy bulk and break it down into smaller amounts. If done in the clean airstream (carefully) your food parcels will last longer.


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#27 pr0wlunwoof

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Posted 27 November 2021 - 11:17 AM

I have high hopes for it. Its taken allot of effort to this point. When I get it finished Im gonna have to make a custom cover for it. My garage is the only space I have to work and it gets dusty fast.

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#28 pr0wlunwoof

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Posted 27 November 2021 - 12:44 PM

Aight its done. Seems to be working as designed. Will maybe get to play with it tomorrow.a9255c66e6cb9b38f5e2fd55870e2e90.jpgeba271a522af9ad917bffc5add942c13.jpg

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#29 pr0wlunwoof

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Posted 28 November 2021 - 11:44 AM

So we are almost ready to give it our first try. Gonna do some Liquid Culture syringes to Liquid Culture 300ml jars to expand my supply. Did a 4% sugar solution with .7 grams of peptone per jar. After the LC transfer I am going to try some Agar to Agar and then Agar to LC jar as well. One will be my Reishi clone I found several weeks back and the other will be a pearl oyster clone I did from my first grow kit. I put a magnetic stirrer in each jar and used injectable tops with micro filter pads covered with a tin foil topper.

This is a bigger test of the flow hood than I should probably try, but if it dont work then I gotta rethink the entire setup. Also I am out of time till probably the week of christmas... So here we go!c1cd1f87416f40faf45559445bbd88ff.jpg

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Edited by pr0wlunwoof, 28 November 2021 - 11:45 AM.

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#30 Myc

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Posted 28 November 2021 - 12:13 PM

You'll want to work fairly close to the filter space.

The run-out on that long table will begin to present eddies in the air currents.

Curious to see how your test turn out. I'm sure you're using a bunsen burner flame to test for even-ness of the laminar airstream.



#31 pr0wlunwoof

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Posted 28 November 2021 - 01:36 PM

You'll want to work fairly close to the filter space.
The run-out on that long table will begin to present eddies in the air currents.
Curious to see how your test turn out. I'm sure you're using a bunsen burner flame to test for even-ness of the laminar airstream.

Well i used a grill lighter and it appears even. I do have an oil lamp let me try that.

Here it is. Not a great video, but the stream seems laminar to me.




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http://cloud.tapatal...1128_134128.mp4

Edited by pr0wlunwoof, 28 November 2021 - 01:51 PM.


#32 Myc

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Posted 28 November 2021 - 02:01 PM

Looks like the flame stayed bent pretty evenly in all positions. Although the top part was out of frame when you were testing that section.

 

To be clear, I'm not a scientist nor do I have any formal lab training. I guess you could say I hacked my way into microbiology through the back door rather than taking classes. That said, I expect that your build will give you countless hours of reliable service.

 

Keep track of pre-filter maintenance and it should last you a lifetime. 


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#33 pr0wlunwoof

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Posted 28 November 2021 - 03:36 PM

Yeah. I felt more and more confident as I used it. Need to work on my movements for sure but I feel it went well. I turned the cabinet on high for half an hour and then setup and and switched it to low and worked one jar at a time dissinfecting between jars. Then i gave everything a good spin and put it up to grow. Fingers crossed but I think it should be good.9461de02d90dc68cd3761732aba33f03.jpg82eb9fe0b0377f8ccec249854217e956.jpg

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Edited by pr0wlunwoof, 28 November 2021 - 07:52 PM.


#34 TVCasualty

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Posted 29 November 2021 - 09:02 AM

If you turn the table 90-degrees you could work closer to it, which might not be a big deal since it looks like it's working as-is, but it might give you more working space. You don't want the air to go over any part of your hand or arm before it contacts any agar or grain (etc.), and sitting to the side of the filter makes that harder to avoid.

 

FWIW, you don't have to have all the jars in the flow of filtered air until you're opening them, so having the table oriented the other way gives you more room to move as you work while the other jars are off to the sides (being covered with foil adds an additional layer of protection to them if you only remove it in front of the filter).

 

 

Since you're working in a space that's not dedicated to sterile tissue culture you could build a "lab" to work in made out of PVC pipe and plastic sheeting that breaks down for storage (when not in use; don't cement the fittings). If you have trouble with contamination (since garages are pretty dusty/dirty) it's an option to consider.


Edited by TVCasualty, 29 November 2021 - 09:03 AM.

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#35 pr0wlunwoof

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Posted 29 November 2021 - 09:20 AM

If you turn the table 90-degrees you could work closer to it, which might not be a big deal since it looks like it's working as-is, but it might give you more working space. You don't want the air to go over any part of your hand or arm before it contacts any agar or grain (etc.), and sitting to the side of the filter makes that harder to avoid.

 

FWIW, you don't have to have all the jars in the flow of filtered air until you're opening them, so having the table oriented the other way gives you more room to move as you work while the other jars are off to the sides (being covered with foil adds an additional layer of protection to them if you only remove it in front of the filter).

 

 

Since you're working in a space that's not dedicated to sterile tissue culture you could build a "lab" to work in made out of PVC pipe and plastic sheeting that breaks down for storage (when not in use; don't cement the fittings). If you have trouble with contamination (since garages are pretty dusty/dirty) it's an option to consider.

I do like the idea of the PVC Lab\tent idea. I may do that. Changing orientation would also be an option I just need to build a permanent dolly system for the Cabinet and the table so I can adjust with ease. I should see in a few days if I have any contamination on the agar plates I did transfers on. If that goes well I am fairly confident the LC to LC is rock solid. 



#36 rockyfungus

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Posted 29 November 2021 - 04:26 PM

Make sure to always work a few inches from top and bottom. Mine seems fine on sides but I stay dead center if I can.

Just leave a plate open in front for 30-1 hour.

#37 pr0wlunwoof

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Posted 04 December 2021 - 01:43 PM

Got the perminant stand and dust cover done today. We in buisness now. Also put casters on my work table now I can configure and reconfigure as needed.3aa9d6321a008d956c91143f9092a96b.jpg02b93363ee2c88c6ca673d6a2605d0c5.jpg

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#38 rockyfungus

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Posted 04 December 2021 - 02:23 PM

Duct tape and tarp...Dude, get some nice fabric and either figure how to sow or find a ladyfriend to make you a nicer cover.
Or is that staying in the elements?


Edited by rockyfungus, 04 December 2021 - 02:23 PM.


#39 Myc

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Posted 04 December 2021 - 10:57 PM

Eh.....the tarp material is a pretty decent choice. I use 6ml heavy plastic.

 

You wouldn't want to use a particle generator - fabric in particular - to cover the unit.

Not bashin' - just sayin'

 

If kept out of direct sunlight - plastics don't tend to generate large particle loads. Trust me, I have many years and many hours of experience building clean rooms.

 

Tyvek would be the best material to use. Stitched would be awesome - but duct tape will last forever too.

 

I wish I had a view on the use of this tool. A fly on the wall's perspective - so to speak, I'll be interested to see where it takes you in the next 20 years or so. You're thoroughly down the rabbit hole now. Possessing such a powerful tool will lead to some forward thinking. There are applications that you do not yet realize (beyond storing cheese - LOL).



#40 pr0wlunwoof

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Posted 05 December 2021 - 12:03 PM

Well.... yeah the tarp cover is probably the jankiest part of it all, but it is what I had on hand and I only had a a few minutes after fabricating the rolling stand and putting casters on the work table to accomplish it. Wife was about done with me "playing". The tarp was brand new in the package from when harbor freight use to give them out for free. The cover will do for now. Mainley keeps the crud off it in the garage. I dont have another usable space, so I am trying to make what I have work.

All the LC and Agar transfers I did are looking good, no signs of infection. I have another test today with 11 grain to bulk transfers. I just finished my pasturizing of the fuel pellets so it has to sit for an hour or so and then cool down.

So far my teks are working. Here are some king oysters in the martha tent I just got up and running: 3ad17ecfa476e8b96ad0918e96148a27.jpg

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