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Chia Seed Agar


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#21 Salty117

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Posted 20 November 2021 - 11:59 PM

I've got a batch in the PC now of just Chia Seed and agar agar powder that I put together before I seen your post Rockyfungus but considering those digits, you may be onto a better track suggesting that I further reduce the Chia Seed content. Since I already have this batch put together, I'm still going to run it and see it through (25g chia seed, 8g agar agar, 500ml boiling water). I'm determined to get this dialed in so I can really cut back on my agar content. Even if it comes down to only using 5~10 grams chia seed per batch... better yet!


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#22 rockyfungus

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Posted 21 November 2021 - 10:06 AM

Does your package say how many grams or stupid cups for it to gel?
My chia is old and just in a spice jar. 



#23 Salty117

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Posted 21 November 2021 - 11:26 AM

It doesn't say how much is needed to thicken the water, just gives a recipe for chia seed blueberry muffins. For those that intend to ingest the chia seed water and want it to be on the thinner side, I found a recommendation for 3 cups of water per 1/3 cup of chia seeds, a 9:1 water to chia seed ratio. Gonna see if I can find a measuring cup and figure out what the weight of that is in Chia.



#24 Salty117

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Posted 21 November 2021 - 11:40 AM

A 1/3 cup of chia seeds, in weight, comes to 56 grams. I'm using a little under half of that in 500ml of water at the time. A cup of water is around 236.5ml.. so 3 cups for a "thinner" mixture (again this is a recipe for if someone is intending to ingest) will run up to 709.5ml.

 

I poured the chia seed agar I mixed late last night, normally I would wait a few days to ensure that everything poured clean with no contamination, but since these are just test batches anyway, I'm not too worried about it. When they cooled to room temp I went ahead and made afew transfers onto some plates from the first batch and some from PDYA cups.

 

I will note as well that the smell of chia seed agar is similar to that of green tea.


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#25 Salty117

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Posted 27 November 2021 - 05:55 PM

Some mycelium is enjoying the latest Chia Seed recipe, others not so much.. the Pan Cyan "Rainbow Eucalyptus" is not digging it, and neither are the Tampanensis and Galindoi (ATL#7) cultures.

 

The Wild TN Cube mycelium, and Ps. Natalensis cultures are having a nice little party in their cups.

 

Wild TN Cube

Wild TN Cube Chia Seed Test Batch 2 Resized.jpg

Wild TN Cube Chia Seed Test Batch 2 Resized 2.jpg

 

Ps. Natalensis

Ps Natalensis Chia Seed Agar Test Batch 2(a) resized.jpg

Ps Natalensis Chia Seed Agar Test Batch 2(b) resized.jpg

 

Ps Natalensis Chia Seed Agar Test Batch 2(c) resized.jpg

Ps Natalensis Chia Seed Agar Test Batch 2(d) resized.jpg

 

I'm planning to run another batch soon, going to reduce the chia seed content to 15g from 25g, and I'm going to leave the agar at 8g for the time being since I'm reducing the chia seed variable. If the density is adequate I'm going to reduce the agar agar content to 6g.

 

Considering Rockyfungus' preferred agar content, I have little reason to be suspicious that reducing the agar content to 6g is going to be inadequate. May further reduce chia seed content to 10g, and eventually try to introduce a couple grams of dextrose.


Edited by Salty117, 27 November 2021 - 06:03 PM.

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#26 Microbe

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Posted 02 December 2021 - 07:40 AM

That agar looks nasty AF lol. I like how simple those little condiment cups are being used for agar. How are you sterilizing them?
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#27 Arathu

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Posted 02 December 2021 - 07:53 AM

Good work Salty.....I should photograph some of the "experimental" things I do.....instead of just "pretty" pictures..... :rolleyes: 

 

@Microbe.....No way dude.....YOU and NEEDLES, others too, got me on GLASS.........no cups, no jelly jars, no Ziploc containers........it's glass and sterilizers for you young man!

 

Hahahahaha.......I can't even believe that I saw a question about it......You and Sandy, Needles, several others in fact....... and I thank you for it!

 

Hi Microbe! Good to see you around......

 

A


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#28 Salty117

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Posted 02 December 2021 - 08:26 AM

That agar looks nasty AF lol. I like how simple those little condiment cups are being used for agar. How are you sterilizing them?

 

The condiment cups come sterile out of the packaging, or at the very least sanitary enough for food use. Out of the last 100 pours I have had maybe 4 or 5 contaminate, and that was likely due to the lid to the cup being outside the laminar flow for a moment for one reason or another. It also eliminates the need for parafilm, but also not all lids seem to fit snugly to the cups, and let enough air exchange to occur to dry out the agar.

 

Edit: To elaborate, I have only had 2 lids for condiment cups of the last few hundred not fit right and dry the agar out.

 

I do have 60ct 60mm borosilicate glass petris and parafilm, but I haven't really begun to use them quite yet. If I'm being honest though, the condiment cups are quite convenient. Especially since I my laminar flow hood is on the smaller size of 1'x1'

 

@Arathu that sounds like a good idea! I love checking out the neat things people are up to around here lol


Edited by Salty117, 02 December 2021 - 09:28 AM.

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#29 Microbe

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Posted 03 December 2021 - 08:29 AM

Good work Salty.....I should photograph some of the "experimental" things I do.....instead of just "pretty" pictures..... :rolleyes:

@Microbe.....No way dude.....YOU and NEEDLES, others too, got me on GLASS.........no cups, no jelly jars, no Ziploc containers........it's glass and sterilizers for you young man!

Hahahahaha.......I can't even believe that I saw a question about it......You and Sandy, Needles, several others in fact....... and I thank you for it!

Hi Microbe! Good to see you around......

A

Oh no, im not even considering them, it just appears they are working for him/her. I was more curious how they are not contaminating. I don't even use plastic plates anymore. After several bad agar pours, i scrapped plastic plates and went back to all glass. I found some nice 100 mm plates that are very comparable to pyrex but half the price. I had low expectations but was very surprised when they arrived.

Things are slowing down for me at work, soon i can get a little more active here again. Im glad to see you still here dropping knowledge and hope all is well with you and yours.
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#30 Microbe

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Posted 03 December 2021 - 08:37 AM

That agar looks nasty AF lol. I like how simple those little condiment cups are being used for agar. How are you sterilizing them?


The condiment cups come sterile out of the packaging, or at the very least sanitary enough for food use. Out of the last 100 pours I have had maybe 4 or 5 contaminate, and that was likely due to the lid to the cup being outside the laminar flow for a moment for one reason or another. It also eliminates the need for parafilm, but also not all lids seem to fit snugly to the cups, and let enough air exchange to occur to dry out the agar.

Edit: To elaborate, I have only had 2 lids for condiment cups of the last few hundred not fit right and dry the agar out.

I do have 60ct 60mm borosilicate glass petris and parafilm, but I haven't really begun to use them quite yet. If I'm being honest though, the condiment cups are quite convenient. Especially since I my laminar flow hood is on the smaller size of 1'x1'

@Arathu that sounds like a good idea! I love checking out the neat things people are up to around here lol
Everyone has their own standards for contam rates, with the obvious target of being 0. I give myself up to 3% before i flip my shit. So i would say they are working out for you very well.
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#31 Salty117

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Posted 03 December 2021 - 10:07 AM

They are, I go for 3.25oz condiment cups for more room for the culture to grow while still being easy to handle. They just aren't super space-saving freindly due to their height and "stackability" being limited, which is part of the reason my work space is often cluttered with condiment cups.






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