I'm just getting started in the hobby but have been interested in mycology for years. I have read countless posts, watched videos and read articles about mycology and thanks to all of the community contributions, I finally gotten off the bench!
- Nov 6 - Inoculation Day: Having done some research, I was aware that this is the first milestone to pass without contamination. I was overly cautious with lysol, alcohol, flame sterilization, still air box and paranoia (I mean caution). I inoculated some Uncle Ben's with a GT spore syringe and taped accordingly. What happened over the next several days was an exercise in patience. I proceeded to look, poke, feel and fret over my babies, as any newb would do. At the first signs of life, I was optimistic. I left everything alone until my bag was 50% colonized and tried the ole break and shake. Shortly thereafter, I thought all was lost...until I learned that mycelium bruise too. Who knew?
- Nov 26 - Spawn to Bulk Day: This step was a lot less intimidating than I expected. After cleaning and sanitizing everything, I laid a base of CVG at field capacity into the bottom of a 6qt tote, covered with colonized grain and covered again with CVG before closing the lid. I also added a seedling mat to keep everything nice and comfortable. I also took the time to transfer some "leftover" grains to new bags of rice. Now I have my next set colonizing as well.
So, that's where I am at now. Waiting and reading.
Question: Is it possible/advisable to clone directly to grain or does the cloned mycelium need to be on agar first?